BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1688019 1 dTomato dTomato, red fluorescent protein 2015-09-14T11:00:00Z 2015-09-15T12:53:18Z dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed. The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm. false false _2106_ 27118 27118 9 false false Erik Gullberg BBa_K1688004 1 dTomato dTomato (inc RBS) 2015-09-14T11:00:00Z 2015-09-18T10:34:03Z It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011). false false _2106_ 27118 27121 9 false false Erik Gullberg component2457404 1 BBa_B0034 component2457405 1 BBa_K1688019 annotation2457404 1 BBa_B0034 range2457404 1 1 12 annotation2457405 1 BBa_K1688019 range2457405 1 19 726 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1688004_sequence 1 aaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa BBa_K1688019_sequence 1 atggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z