LOCUS BBa_K1688005 3810 bp DNA linear 26-Nov-2024 DEFINITION dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system) ACCESSION BBa_K1688005 VERSION BBa_K1688005.1 FEATURES Location/Qualifiers promoter 1..1268 /label=BBa_J61051 misc_feature 1281..1284 /label=conserved RBS 1277..1288 /label=BBa_B0034 CDS 1295..2002 /label=BBa_K1688019 misc_feature 1277..2002 /label=BBa_K1688004 misc_feature 1..2002 /label=BBa_K1688012 misc_feature 2015..2018 /label=conserved RBS 2011..2022 /label=BBa_B0034 misc_feature 2032..2048 /label=6xHis misc_feature 3618..3809 /label=HlyA export tag CDS 2029..3810 /label=BBa_K1688020 misc_feature 2011..3810 /label=BBa_K1688006 ORIGIN 1 ggccgctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt 61 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg 121 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg 181 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg 241 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc 301 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacgcagca 361 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac 421 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta 481 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca 541 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac 601 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc 661 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg 721 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat 781 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg 841 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg 901 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag 961 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag 1021 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac 1081 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg 1141 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca 1201 atattgtttg ctccgttatc gttattaaca agtcatcaat aaagccatca cgagtaccat 1261 agaggatcta ctagagaaag aggagaaata ctagatggtt agcaagggtg aagaagtgat 1321 taaagaattt atgcgcttta aagttcgtat ggaaggctcg atgaacggtc acgagttcga 1381 gatcgaaggt gagggtgagg gtcgtccgta cgaaggcacc caaaccgcga aactgaaggt 1441 tacgaagggt ggcccgctgc cgtttgcctg ggacatcctg tccccgcagt tcatgtacgg 1501 tagcaaggcg tatgtcaagc atccagcaga cattccggac tacaaaaagc tgagcttccc 1561 ggaaggtttc aaatgggagc gtgtcatgaa ctttgaagat ggcggcttgg ttaccgtgac 1621 ccaagatagc tctctgcaag acggcacctt gatctacaag gtgaagatgc gcggcacgaa 1681 ttttccgccg gacggtccgg tgatgcagaa gaaaaccatg ggttgggaag caagcacgga 1741 gcgtctgtac ccgcgtgatg gtgtcctgaa aggtgagatt caccaggcgc tgaaactgaa 1801 agatggcggc cattacctgg tcgagttcaa gaccatctat atggctaaaa agcctgtgca 1861 actgccgggt tactattatg ttgataccaa actggacatt acgagccata atgaagatta 1921 tactatcgtt gagcagtatg agcgtagcga gggtcgccac cacttgttcc tgtacggtat 1981 ggacgagctg tacaaataat aatactagag aaagaggaga aatactagat gcatcatcat 2041 catcatcata tgcatcaacg tcgtgatttc ttaaaatatt ccgtcgcgct gggtgtggct 2101 tcggctttgc cgctgtggag ccgcgcagta tttgcggcag aacgcccaac gttaccgatc 2161 cctgatttgc tcacgaccga tgcccgtaat cgcattcagt taactattgg cgcaggccag 2221 tccacctttg gcgggaaaac tgcaactacc tggggctata acggcaatct gctggggccg 2281 gcggtgaaat tacagcgcgg caaagcggta acggttgata tctacaacca actgacggaa 2341 gagacaacgt tgcactggca cgggctggaa gtaccgggtg aagtcgacgg cggcccgcag 2401 ggaattattc cgccaggtgg caagcgctcg gtgacgttga acgttgatca acctgccgct 2461 acctgctggt tccatccgca tcagcacggc aaaaccgggc gacaggtggc gatggggctg 2521 gctgggctgg tggtgattga agatgacgag atcctgaaat taatgctgcc aaaacagtgg 2581 ggtatcgatg atgttccggt gatcgttcag gataagaaat ttagcgccga cgggcagatt 2641 gattatcaac tggatgtgat gaccgccgcc gtgggctggt ttggcgatac gttgctgacc 2701 aacggtgcaa tctacccgca acacgctgcc ccgcgtggtt ggctgcgcct gcgtttgctc 2761 aatggctgta atgcccgttc gctcaatttc gccaccagcg acaatcgccc gctgtatgtg 2821 attgccagcg acggtggtct gctacctgaa ccagtgaagg tgagcgaact gccggtgctg 2881 atgggcgagc gttttgaagt gctggtggag gttaacgata acaaaccctt tgacctggtg 2941 acgctgccgg tcagccagat ggggatggcg attgcgccgt ttgataagcc tcatccggta 3001 atgcggattc agccgattgc tattagtgcc tccggtgctt tgccagacac attaagtagc 3061 ctgcctgcgt taccttcgct ggaagggctg acggtacgca agctgcaact ctctatggac 3121 ccgatgctcg atatgatggg gatgcagatg ctaatggaga aatatggcga tcaggcgatg 3181 gccgggatgg atcacagcca gatgatgggc catatggggc acggcaatat gaatcatatg 3241 aaccacggcg ggaagttcga tttccaccat gccaacaaaa tcaacggtca ggcgtttgat 3301 atgaacaagc cgatgtttgc ggcggcgaaa gggcaatacg aacgttgggt tatctctggc 3361 gtgggcgcaa tgatgctgca tccgttccat atccacggca cgcagttccg tatcttgtca 3421 gaaaatggca aaccgccagc ggctcatcgc gcgggctgga aagataccgt taaggtagaa 3481 ggtaatgtca gcgaagtgct ggtgaagttt aatcacgatg caccgaaaga acatgcttat 3541 atggcgcact gccatctgct ggagcatgaa gatacggggc tgatgttagg gtttacggta 3601 ggctctggta cctctggctt agcctatgga agtcagggtg atcttaatcc attaattaat 3661 gaaatcagca aaatcatttc agcagcaggt agcttcgatg ttaaagagga aagaaccgca 3721 gcttctttat tgcagttgtc cggtaatgcc agtgattttt catatggacg gaactcaata 3781 accctgacca catcagcagg tacctaataa //