BBa_K1688005 1 BBa_K1688005 dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system) 2015-09-14T11:00:00Z 2015-09-18T11:15:29Z BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002). false false _2106_ 27118 27118 9 false false Fredrik Lindeberg component2457949 1 BBa_K1688006 component2457945 1 BBa_K1688012 annotation2457945 1 BBa_K1688012 range2457945 1 1 2002 annotation2457949 1 BBa_K1688006 range2457949 1 2011 3810 BBa_K1688006 1 BBa_K1688006 ModLac laccase with His-tag and HlyA export tag (inc RBS) 2015-09-14T11:00:00Z 2015-09-18T11:34:39Z D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag and HlyA export tag. A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. false false _2106_ 27118 27118 9 false The modified CueO was synthesized with iGEM prefix and suffix, RBS(BBa_B0034) and a M-scar after the RBS in order to guarantee the function of the RBS. The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. This is needed for the export tag HlyA , to make it easier for the resulting signal protein to be folded properly. A second KpnI restriction site is put after the HlyA so that the export tag can be removed easily. It has then two stop codons, and a terminator at the end. false Fredrik Lindeberg component2460173 1 BBa_K1688020 component2460172 1 BBa_B0034 annotation2460172 1 BBa_B0034 range2460172 1 1 12 annotation2460173 1 BBa_K1688020 range2460173 1 19 1800 BBa_K1688019 1 dTomato dTomato, red fluorescent protein 2015-09-14T11:00:00Z 2015-09-15T12:53:18Z dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed. The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm. false false _2106_ 27118 27118 9 false false Erik Gullberg BBa_J61051 1 BBa_J61051 [Psal1] 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z non Released HQ 2013 Salicylate promoter and nahR false false _95_ 0 483 95 In stock false nonh false John Anderson BBa_K1688012 1 BBa_K1688012 dTomato (inc RBS, NahR/Psal promoter system) 2015-09-14T11:00:00Z 2015-09-18T11:13:59Z Regulatory NahR construct with RBS(B0034) and dTomato Red Fluorescent Protein as a reporter. false false _2106_ 27118 27118 9 false false Fredrik Lindeberg component2457930 1 BBa_J61051 component2457934 1 BBa_K1688004 annotation2457934 1 BBa_K1688004 range2457934 1 1277 2002 annotation2457930 1 BBa_J61051 range2457930 1 1 1268 BBa_K1688004 1 dTomato dTomato (inc RBS) 2015-09-14T11:00:00Z 2015-09-18T10:34:03Z It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011). false false _2106_ 27118 27121 9 false false Erik Gullberg component2457404 1 BBa_B0034 component2457405 1 BBa_K1688019 annotation2457405 1 BBa_K1688019 range2457405 1 19 726 annotation2457404 1 BBa_B0034 range2457404 1 1 12 BBa_K1688020 1 ModLac ModLac laccase with His-tag and HlyA export tag 2015-09-14T11:00:00Z 2015-09-15T01:47:52Z D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag and HlyA export tag. A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. false false _2106_ 27118 27118 9 false The modified CueO was synthesized with iGEM prefix and suffix, RBS(BBa_B0034) and a M-scar after the RBS in order to guarantee the function of the RBS. Two Nsil restriction sites were put before and after a 6xHis-tag so that the enzyme could be purified with an IMAC and so that the tag could be removed. The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. This is needed for the export tag HlyA , to make it easier for the resulting signal protein to be folded properly. A second KpnI restriction site is put after the HlyA so that the export tag can be removed easily. It has then two stop codons, and a terminator at the end. false Fredrik Lindeberg annotation2460174 1 6xHis range2460174 1 4 20 annotation2461027 1 HlyA export tag range2461027 1 1590 1781 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1688004_sequence 1 aaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa BBa_J61051_sequence 1 ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatc BBa_K1688006_sequence 1 aaagaggagaaatactagatgcatcatcatcatcatcatatgcatcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcaggtacctaataa BBa_K1688005_sequence 1 ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataatactagagaaagaggagaaatactagatgcatcatcatcatcatcatatgcatcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcaggtacctaataa BBa_K1688012_sequence 1 ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa BBa_K1688020_sequence 1 atgcatcatcatcatcatcatatgcatcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcaggtacctaataa BBa_K1688019_sequence 1 atggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z