LOCUS BBa_K1688007 1843 bp DNA linear 27-Dec-2024 DEFINITION ModLac laccase with His-tag and HlyA export tag (inc RBS and J23110 promoter) ACCESSION BBa_K1688007 VERSION BBa_K1688007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 65..81 /label=6xHis misc_feature 1651..1842 /label=HlyA export tag CDS 62..1843 /label=BBa_K1688020 misc_feature 44..1843 /label=BBa_K1688006 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgcatcat catcatcatc atatgcatca acgtcgtgat ttcttaaaat attccgtcgc 121 gctgggtgtg gcttcggctt tgccgctgtg gagccgcgca gtatttgcgg cagaacgccc 181 aacgttaccg atccctgatt tgctcacgac cgatgcccgt aatcgcattc agttaactat 241 tggcgcaggc cagtccacct ttggcgggaa aactgcaact acctggggct ataacggcaa 301 tctgctgggg ccggcggtga aattacagcg cggcaaagcg gtaacggttg atatctacaa 361 ccaactgacg gaagagacaa cgttgcactg gcacgggctg gaagtaccgg gtgaagtcga 421 cggcggcccg cagggaatta ttccgccagg tggcaagcgc tcggtgacgt tgaacgttga 481 tcaacctgcc gctacctgct ggttccatcc gcatcagcac ggcaaaaccg ggcgacaggt 541 ggcgatgggg ctggctgggc tggtggtgat tgaagatgac gagatcctga aattaatgct 601 gccaaaacag tggggtatcg atgatgttcc ggtgatcgtt caggataaga aatttagcgc 661 cgacgggcag attgattatc aactggatgt gatgaccgcc gccgtgggct ggtttggcga 721 tacgttgctg accaacggtg caatctaccc gcaacacgct gccccgcgtg gttggctgcg 781 cctgcgtttg ctcaatggct gtaatgcccg ttcgctcaat ttcgccacca gcgacaatcg 841 cccgctgtat gtgattgcca gcgacggtgg tctgctacct gaaccagtga aggtgagcga 901 actgccggtg ctgatgggcg agcgttttga agtgctggtg gaggttaacg ataacaaacc 961 ctttgacctg gtgacgctgc cggtcagcca gatggggatg gcgattgcgc cgtttgataa 1021 gcctcatccg gtaatgcgga ttcagccgat tgctattagt gcctccggtg ctttgccaga 1081 cacattaagt agcctgcctg cgttaccttc gctggaaggg ctgacggtac gcaagctgca 1141 actctctatg gacccgatgc tcgatatgat ggggatgcag atgctaatgg agaaatatgg 1201 cgatcaggcg atggccggga tggatcacag ccagatgatg ggccatatgg ggcacggcaa 1261 tatgaatcat atgaaccacg gcgggaagtt cgatttccac catgccaaca aaatcaacgg 1321 tcaggcgttt gatatgaaca agccgatgtt tgcggcggcg aaagggcaat acgaacgttg 1381 ggttatctct ggcgtgggcg caatgatgct gcatccgttc catatccacg gcacgcagtt 1441 ccgtatcttg tcagaaaatg gcaaaccgcc agcggctcat cgcgcgggct ggaaagatac 1501 cgttaaggta gaaggtaatg tcagcgaagt gctggtgaag tttaatcacg atgcaccgaa 1561 agaacatgct tatatggcgc actgccatct gctggagcat gaagatacgg ggctgatgtt 1621 agggtttacg gtaggctctg gtacctctgg cttagcctat ggaagtcagg gtgatcttaa 1681 tccattaatt aatgaaatca gcaaaatcat ttcagcagca ggtagcttcg atgttaaaga 1741 ggaaagaacc gcagcttctt tattgcagtt gtccggtaat gccagtgatt tttcatatgg 1801 acggaactca ataaccctga ccacatcagc aggtacctaa taa //