BBa_K1688009
type: ComponentDefinition
persistent identity: BBa K1688009
display id: BBa_K1688009
version: 1
was generated by: iGEM to SBOL conversion
description: dTomato, ModLac and Dioxygenase (inc RBS, NahR/Psal promoter system)
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T11:16:48Z
owned by: james
owned by: myers
mutable description: BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato (BBa_K1688004), the modified laccase, ModLac (BBa_K1688006), and a dioxygenase.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Fredrik Lindeberg
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1688022
component: BBa_K1688005
sequence annotation: BBa_K1688005
sequence annotation: BBa_K1688022
sequence: https://synbiohub.org/public/igem/BBa_K1688009_sequence/1

BBa_K1688022
type: Component
persistent identity: component2461152
display id: component2461152
version: 1
top level: BBa_K1688009
definition: Dioxy
access: public

BBa_K1688005
type: Component
persistent identity: component2461142
display id: component2461142
version: 1
top level: BBa_K1688009
definition: BBa_K1688005
access: public

BBa_K1688005
type: SequenceAnnotation
persistent identity: annotation2461142
display id: annotation2461142
version: 1
top level: BBa_K1688009
location: https://synbiohub.org/public/igem/BBa_K1688009/annotation2461142/range2461142/1
component: BBa_K1688005

https://synbiohub.org/public/igem/BBa_K1688009/annotation2461142/range2461142/1
type: Range
persistent identity: range2461142
display id: range2461142
version: 1
top level: BBa_K1688009
direction: direction/reverse
start: 1
end: 3810
orientation: inline

BBa_K1688022
type: SequenceAnnotation
persistent identity: annotation2461152
display id: annotation2461152
version: 1
top level: BBa_K1688009
location: https://synbiohub.org/public/igem/BBa_K1688009/annotation2461152/range2461152/1
component: BBa_K1688022

https://synbiohub.org/public/igem/BBa_K1688009/annotation2461152/range2461152/1
type: Range
persistent identity: range2461152
display id: range2461152
version: 1
top level: BBa_K1688009
direction: direction/reverse
start: 3819
end: 4977
orientation: inline

BBa_K1688005
type: ComponentDefinition
persistent identity: BBa K1688005
display id: BBa_K1688005
version: 1
was generated by: iGEM to SBOL conversion
description: dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:15:29Z
owned by: james
owned by: myers
mutable description: BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002).
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Fredrik Lindeberg
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1688012
component: BBa_K1688006
sequence annotation: BBa_K1688012
sequence annotation: BBa_K1688006
sequence: https://synbiohub.org/public/igem/BBa_K1688005_sequence/1

BBa_K1688012
type: Component
persistent identity: component2457945
display id: component2457945
version: 1
top level: BBa_K1688005
definition: BBa_K1688012
access: public

BBa_K1688006
type: Component
persistent identity: component2457949
display id: component2457949
version: 1
top level: BBa_K1688005
definition: BBa_K1688006
access: public

BBa_K1688012
type: SequenceAnnotation
persistent identity: annotation2457945
display id: annotation2457945
version: 1
top level: BBa_K1688005
location: https://synbiohub.org/public/igem/BBa_K1688005/annotation2457945/range2457945/1
component: BBa_K1688012

https://synbiohub.org/public/igem/BBa_K1688005/annotation2457945/range2457945/1
type: Range
persistent identity: range2457945
display id: range2457945
version: 1
top level: BBa_K1688005
direction: direction/reverse
start: 1
end: 2002
orientation: inline

BBa_K1688006
type: SequenceAnnotation
persistent identity: annotation2457949
display id: annotation2457949
version: 1
top level: BBa_K1688005
location: https://synbiohub.org/public/igem/BBa_K1688005/annotation2457949/range2457949/1
component: BBa_K1688006

https://synbiohub.org/public/igem/BBa_K1688005/annotation2457949/range2457949/1
type: Range
persistent identity: range2457949
display id: range2457949
version: 1
top level: BBa_K1688005
direction: direction/reverse
start: 2011
end: 3810
orientation: inline

Dioxy
type: ComponentDefinition
persistent identity: BBa K1688022
display id: BBa_K1688022
version: 1
was generated by: iGEM to SBOL conversion
description: Catechol 1,2 Dioxygenase + HlyA export tag
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T11:10:54Z
mutable provenance: Restriction free cloning.
owned by: james
owned by: myers
mutable description: Catechol 1,2 dioxygenase (BBa_K1092003) fused to HlyA export tag (BBa_K554002), but with an introduced frameshift due to improper assembly.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27552
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Primers for restriction-free cloning were designed in such a way as to remove the STOP codon of the catechol 1,2 dioxygenase and the scar left from 3A assembly. However, due to a design error, an AG insert between the two parts was introduced, resulting in a stop codon at the beginning of the HlyA sequence.
star: false
creator: Delyan Georgiev
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: T7 Promoter
sequence annotation: RBS
sequence annotation: 3A Scar
sequence annotation: START
sequence annotation: Dioxygenase
sequence annotation: Frameshift insertion
sequence annotation: HlyA C-tag
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1688022_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2460969
display id: annotation2460969
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2460969/range2460969/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1688022/annotation2460969/range2460969/1
type: Range
persistent identity: range2460969
display id: range2460969
version: 1
top level: Dioxy
direction: direction/forward
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2461087
display id: annotation2461087
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461087/range2461087/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461087/range2461087/1
type: Range
persistent identity: range2461087
display id: range2461087
version: 1
top level: Dioxy
direction: direction/forward
start: 21
end: 32
orientation: inline

3A Scar
type: SequenceAnnotation
persistent identity: annotation2461088
display id: annotation2461088
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461088/range2461088/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461088/range2461088/1
type: Range
persistent identity: range2461088
display id: range2461088
version: 1
top level: Dioxy
direction: direction/forward
start: 33
end: 38
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2460971
display id: annotation2460971
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2460971/range2460971/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1688022/annotation2460971/range2460971/1
type: Range
persistent identity: range2460971
display id: range2460971
version: 1
top level: Dioxy
direction: direction/forward
start: 39
end: 41
orientation: inline

Dioxygenase
type: SequenceAnnotation
persistent identity: annotation2460970
display id: annotation2460970
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2460970/range2460970/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1688022/annotation2460970/range2460970/1
type: Range
persistent identity: range2460970
display id: range2460970
version: 1
top level: Dioxy
direction: direction/forward
start: 39
end: 971
orientation: inline

Frameshift insertion
type: SequenceAnnotation
persistent identity: annotation2461022
display id: annotation2461022
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461022/range2461022/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461022/range2461022/1
type: Range
persistent identity: range2461022
display id: range2461022
version: 1
top level: Dioxy
direction: direction/forward
start: 972
end: 973
orientation: inline

HlyA C-tag
type: SequenceAnnotation
persistent identity: annotation2461019
display id: annotation2461019
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461019/range2461019/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461019/range2461019/1
type: Range
persistent identity: range2461019
display id: range2461019
version: 1
top level: Dioxy
direction: direction/forward
start: 974
end: 1153
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2461020
display id: annotation2461020
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461020/range2461020/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461020/range2461020/1
type: Range
persistent identity: range2461020
display id: range2461020
version: 1
top level: Dioxy
direction: direction/forward
start: 1154
end: 1156
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2461021
display id: annotation2461021
version: 1
top level: Dioxy
location: https://synbiohub.org/public/igem/BBa_K1688022/annotation2461021/range2461021/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1688022/annotation2461021/range2461021/1
type: Range
persistent identity: range2461021
display id: range2461021
version: 1
top level: Dioxy
direction: direction/forward
start: 1157
end: 1159
orientation: inline

BBa_K1688006
type: ComponentDefinition
persistent identity: BBa K1688006
display id: BBa_K1688006
version: 1
was generated by: iGEM to SBOL conversion
description: ModLac laccase with His-tag and HlyA export tag (inc RBS)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:34:39Z
mutable provenance: D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag and HlyA export tag.
owned by: james
owned by: myers
mutable description: A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The modified CueO was synthesized with iGEM prefix and suffix, RBS(BBa_B0034) and a M-scar after the RBS in order to guarantee the function of the RBS. The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. This is needed for the export tag HlyA , to make it easier for the resulting signal protein to be folded properly. A second KpnI restriction site is put after the HlyA so that the export tag can be removed easily. It has then two stop codons, and a terminator at the end.
star: false
creator: Fredrik Lindeberg
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1688020
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1688020
sequence: https://synbiohub.org/public/igem/BBa_K1688006_sequence/1

BBa_K1688020
type: Component
persistent identity: component2460173
display id: component2460173
version: 1
top level: BBa_K1688006
definition: ModLac
access: public

BBa_B0034
type: Component
persistent identity: component2460172
display id: component2460172
version: 1
top level: BBa_K1688006
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2460172
display id: annotation2460172
version: 1
top level: BBa_K1688006
location: https://synbiohub.org/public/igem/BBa_K1688006/annotation2460172/range2460172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1688006/annotation2460172/range2460172/1
type: Range
persistent identity: range2460172
display id: range2460172
version: 1
top level: BBa_K1688006
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1688020
type: SequenceAnnotation
persistent identity: annotation2460173
display id: annotation2460173
version: 1
top level: BBa_K1688006
location: https://synbiohub.org/public/igem/BBa_K1688006/annotation2460173/range2460173/1
component: BBa_K1688020

https://synbiohub.org/public/igem/BBa_K1688006/annotation2460173/range2460173/1
type: Range
persistent identity: range2460173
display id: range2460173
version: 1
top level: BBa_K1688006
direction: direction/reverse
start: 19
end: 1800
orientation: inline

BBa_K1688012
type: ComponentDefinition
persistent identity: BBa K1688012
display id: BBa_K1688012
version: 1
was generated by: iGEM to SBOL conversion
description: dTomato (inc RBS, NahR/Psal promoter system)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:13:59Z
owned by: james
owned by: myers
mutable description: Regulatory NahR construct with RBS(B0034) and dTomato Red Fluorescent Protein as a reporter.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Fredrik Lindeberg
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J61051
component: BBa_K1688004
sequence annotation: BBa_J61051
sequence annotation: BBa_K1688004
sequence: https://synbiohub.org/public/igem/BBa_K1688012_sequence/1

BBa_J61051
type: Component
persistent identity: component2457930
display id: component2457930
version: 1
top level: BBa_K1688012
definition: BBa_J61051
access: public

BBa_K1688004
type: Component
persistent identity: component2457934
display id: component2457934
version: 1
top level: BBa_K1688012
definition: dTomato
access: public

BBa_J61051
type: SequenceAnnotation
persistent identity: annotation2457930
display id: annotation2457930
version: 1
top level: BBa_K1688012
location: https://synbiohub.org/public/igem/BBa_K1688012/annotation2457930/range2457930/1
component: BBa_J61051

https://synbiohub.org/public/igem/BBa_K1688012/annotation2457930/range2457930/1
type: Range
persistent identity: range2457930
display id: range2457930
version: 1
top level: BBa_K1688012
direction: direction/reverse
start: 1
end: 1268
orientation: inline

BBa_K1688004
type: SequenceAnnotation
persistent identity: annotation2457934
display id: annotation2457934
version: 1
top level: BBa_K1688012
location: https://synbiohub.org/public/igem/BBa_K1688012/annotation2457934/range2457934/1
component: BBa_K1688004

https://synbiohub.org/public/igem/BBa_K1688012/annotation2457934/range2457934/1
type: Range
persistent identity: range2457934
display id: range2457934
version: 1
top level: BBa_K1688012
direction: direction/reverse
start: 1277
end: 2002
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

ModLac
type: ComponentDefinition
persistent identity: BBa K1688020
display id: BBa_K1688020
version: 1
was generated by: iGEM to SBOL conversion
description: ModLac laccase with His-tag and HlyA export tag
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T01:47:52Z
mutable provenance: D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag and HlyA export tag.
owned by: james
owned by: myers
mutable description: A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The modified CueO was synthesized with iGEM prefix and suffix, RBS(BBa_B0034) and a M-scar after the RBS in order to guarantee the function of the RBS. Two Nsil restriction sites were put before and after a 6xHis-tag so that the enzyme could be purified with an IMAC and so that the tag could be removed. The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. This is needed for the export tag HlyA , to make it easier for the resulting signal protein to be folded properly. A second KpnI restriction site is put after the HlyA so that the export tag can be removed easily. It has then two stop codons, and a terminator at the end.
star: false
creator: Fredrik Lindeberg
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6xHis
sequence annotation: HlyA export tag
sequence: https://synbiohub.org/public/igem/BBa_K1688020_sequence/1

6xHis
type: SequenceAnnotation
persistent identity: annotation2460174
display id: annotation2460174
version: 1
top level: ModLac
location: https://synbiohub.org/public/igem/BBa_K1688020/annotation2460174/range2460174/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1688020/annotation2460174/range2460174/1
type: Range
persistent identity: range2460174
display id: range2460174
version: 1
top level: ModLac
direction: direction/forward
start: 4
end: 20
orientation: inline

HlyA export tag
type: SequenceAnnotation
persistent identity: annotation2461027
display id: annotation2461027
version: 1
top level: ModLac
location: https://synbiohub.org/public/igem/BBa_K1688020/annotation2461027/range2461027/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1688020/annotation2461027/range2461027/1
type: Range
persistent identity: range2461027
display id: range2461027
version: 1
top level: ModLac
direction: direction/forward
start: 1590
end: 1781
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688004
display id: BBa_K1688004
version: 1
was generated by: iGEM to SBOL conversion
description: dTomato (inc RBS)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T10:34:03Z
mutable provenance: It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed
owned by: james
owned by: myers
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011).
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27118
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Erik Gullberg
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_K1688019
sequence annotation: BBa_B0034
sequence annotation: BBa_K1688019
sequence: https://synbiohub.org/public/igem/BBa_K1688004_sequence/1

BBa_B0034
type: Component
persistent identity: component2457404
display id: component2457404
version: 1
top level: dTomato
definition: BBa_B0034
access: public

BBa_K1688019
type: Component
persistent identity: component2457405
display id: component2457405
version: 1
top level: dTomato
definition: dTomato
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2457404
display id: annotation2457404
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
type: Range
persistent identity: range2457404
display id: range2457404
version: 1
top level: dTomato
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1688019
type: SequenceAnnotation
persistent identity: annotation2457405
display id: annotation2457405
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
component: BBa_K1688019

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
type: Range
persistent identity: range2457405
display id: range2457405
version: 1
top level: dTomato
direction: direction/reverse
start: 19
end: 726
orientation: inline

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: iGEM to SBOL conversion
description: [Psal1]
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: non
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Salicylate promoter and nahR
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: nonh
star: false
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

dTomato
type: ComponentDefinition
persistent identity: BBa K1688019
display id: BBa_K1688019
version: 1
was generated by: iGEM to SBOL conversion
description: dTomato, red fluorescent protein
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:53:18Z
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm.
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Erik Gullberg
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1688019_sequence/1

https://synbiohub.org/public/igem/BBa_K1688009_sequence/1
type: Sequence
persistent identity: BBa K1688009 sequence
display id: BBa_K1688009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688005_sequence/1
type: Sequence
persistent identity: BBa K1688005 sequence
display id: BBa_K1688005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688022_sequence/1
type: Sequence
persistent identity: BBa K1688022 sequence
display id: BBa_K1688022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaatactagatgaccgtgaaaattagccataccgcggatattcaggcgttttttaaccgcgtggcgggcctggatcatgcggaaggcaacccgcgctttaaacagattattctgcgcgtgctacaggataccgcgcgcctgattgaagatctggaaattaccgaagatgaattttggcatgcggtggattatctgaaccgcctgggtggccgcaacgaagcgggcctgctggcggcgggcctgggcattgaacattttctggatctgctacaggatgcgaaagatgcggaagcgggcctgggcggcggcaccccgcgcaccattgaaggcccgctgtatgtggcgggcgcgccgctggcgcagggcgaagcgcgcatggatgatggcaccgatccgggcgtggtgatgtttctacagggccaggtgtttgatgcggatggcaaaccgctggcgggcgcgaccgtggatctgtggcatgcgaacacccagggcacctatagctattttgatagcacccagagcgaatttaacctgcgccgccgcattattaccgatgcggaaggccgctatcgcgcgcgcagcattgtgccgagcggctatggctgcgatccgcagggcccgacccaggaatgcctggatctgctgggccgccatggccagcgcccggcgcatgtgcatttttttattagcgcgccgggccatcgccatctgaccacccagattaactttgcgggcgataaatatctgtgggatgattttgcgtatgcgacccgcgatggcctgattggcgaactgcgctttgtggaagatgcggcggcggcgcgcgatcgcggcgtgcagggcgaacgctttgcggaactgagctttgattttcgcctacagggcgcgaaaagcccggatgcggaagcgcgcagccatcgcccgcgcgcgctacaggaaggcagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688006_sequence/1
type: Sequence
persistent identity: BBa K1688006 sequence
display id: BBa_K1688006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcatcatcatcatcatcatatgcatcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcaggtacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688012_sequence/1
type: Sequence
persistent identity: BBa K1688012 sequence
display id: BBa_K1688012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatctactagagaaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688020_sequence/1
type: Sequence
persistent identity: BBa K1688020 sequence
display id: BBa_K1688020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcatcatcatcatcatcatatgcatcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgcaatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggctgatgttagggtttacggtaggctctggtacctctggcttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcaggtacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688004_sequence/1
type: Sequence
persistent identity: BBa K1688004 sequence
display id: BBa_K1688004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61051_sequence/1
type: Sequence
persistent identity: BBa J61051 sequence
display id: BBa_J61051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgccggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgcaagcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagaggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1688019_sequence/1
type: Sequence
persistent identity: BBa K1688019 sequence
display id: BBa_K1688019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagcaagggtgaagaagtgattaaagaatttatgcgctttaaagttcgtatggaaggctcgatgaacggtcacgagttcgagatcgaaggtgagggtgagggtcgtccgtacgaaggcacccaaaccgcgaaactgaaggttacgaagggtggcccgctgccgtttgcctgggacatcctgtccccgcagttcatgtacggtagcaaggcgtatgtcaagcatccagcagacattccggactacaaaaagctgagcttcccggaaggtttcaaatgggagcgtgtcatgaactttgaagatggcggcttggttaccgtgacccaagatagctctctgcaagacggcaccttgatctacaaggtgaagatgcgcggcacgaattttccgccggacggtccggtgatgcagaagaaaaccatgggttgggaagcaagcacggagcgtctgtacccgcgtgatggtgtcctgaaaggtgagattcaccaggcgctgaaactgaaagatggcggccattacctggtcgagttcaagaccatctatatggctaaaaagcctgtgcaactgccgggttactattatgttgataccaaactggacattacgagccataatgaagattatactatcgttgagcagtatgagcgtagcgagggtcgccaccacttgttcctgtacggtatggacgagctgtacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z