BBa_K1688018 1 BBa_K1688018 Nah7 - Upper naphthalene breakdown pathway mutagenized 2015-09-14T11:00:00Z 2015-09-18T11:19:06Z Nah7 plasmid from the PpG7 strain of Pseudomonas putida. Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate. false false _2106_ 27796 27453 9 false The pathway contains two endogenous restriction sites for the enzyme PstI that were removed with site-directed mutagenesis. false Mikael Andersson Sch??nn BBa_K1688018_sequence 1 gccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcggagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccagtgaccacttttacaaatggtgactgctaccgcggtcaccattaatcaaaagggaatgtaagtgtatgggcaatcaacaagtcgtttcgataaccggtgccggctcaggaatcggtctcgaactggttcgatcctttaagttggccggttattgcgtatccgctctcgtgcgaaacgaggagcaagaggcgcttctttgcaatgagttcaaggacgcactcgagatcgtagtgggcgatgtccgggaccacgcgacaaatgagaagctgataaagcagacaattgatagattcggtcatctcgattgtttcatcgcaaatgccggtatttgggattacatgctgaatatcgaagagccttgggagaaaatctcgagcagttttgacgaaatattcgacatcaatgtcaagagctatttcagtggcatcagtgccgccctgcccgagctgaaaaagactaacggatcagtggtgatgaccgcttcggtgtcgtcccatgcggtcggtggtggtggttcttgctacatcgccagcaagcatgcggtgctgggtatggtcaaggctttggcctacgaattggcccccgaaattcgcgtgaacgctgtttcgcctggcggcaccgtgacgtctctgtgcggtcccgctagcgccggtttcgacaaaatgcacatgaaagacatgcccggcatcgacgatatgatcaaaggtctcacacctcttgggtttgcagccaagcccgaagacgtggtggcaccctatctgttgctggcttcgcgaaagcaaggaaaattcatcactggcaccgtgattagcattgatggcggtatggcgctcggtcgcaagtgagcttgtagccgatcagaagttagacacattttcaggtgacgccccatgaagacaaaactgtttatcaacaacgcctggatcgattccagtgaccagcagaccttcgagcgcaagcaccccgtcagcagcgaggtgatgactgagagcgcaaacgccacggtgacggacgcgataaaggcggcgcaagcggccgaggaggcattcaagacctggaaggacgtcggaccttcggagcgtcgccgccttctcctgaaggtcgccgatgtcatggaaagtaaaacaccgaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgggaggctgcctcgctggccacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgaccctacgtcagccggtcggtccgatcctaagcatcgttccatggaacggcaccgcagtgctggcggcacgagccatcgcttatccgctggtctgtggcaacacggtagtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaactacctcaactcttcgcctgaccgttcgcccgagatcgccgacgcactgatctccgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaggccgcgcaacacctcaagcgctgcctactggagctcggcggcaagtccccgcttattgttttagatgacgcagacatcaacgcggcggtaaaggcagcggtgttcggtagctttctattccaaggtcagatctgcatgtccactgagcgcttggtggttgatgagaagatcgccgacgaatttgtcgctaggtttgtcgaaaaaactgagcgcttgagcgtgggcgacccgtgcctaactggcgactgcatcatcggcccgatggtctcgccaaattcgggcgagcggatcaatggtttgttcaaggacgcgatagataaaggggccaaagttgtttgcggcggcatggcccagggtgcggtcatgccggccacgatcctggatcacgtcaaatctgacatgcggatctacgatgaggagacctttggtcccatcaccgtggtgatccgttgcaaaggcgaagcagaggccatccgcattgccaacgacagcgtttatggtctgtcgtcgggcgtgtttggccgcgacatcaaccgtgctctgcgagtgggtatgtccatcgaatatggttgcgtacacatcaacggctcgaccgtccagaacgaggcgcaggctccttacggaggtaccaagaacaccggctacgggcgcttcgacggccgtgccgtgatcgacgagttcacagagctcaagtggctgaccattgaaccattcgagcagcaatatcccttctgaaagcactaactccaaggaatcaaactatgagtaagcaagctgcggttatcgagctcggatacatgggcatctcggtcaaggatcctgatgcgtggaaatcgtttgccatgaatatgctgggtctgcaagttctggatgagggtgagaaggaccgtttctatctgcggatggattactggcaccatcggatcgtagttcatcacagcgcagaggacgacttggaatacctaggctggcgtgtagccggcaagccggagttcgaagctctggggcaaaagctcattgatgccggttacaagatccgtgtctgcgacaaagttgaggctcaggagcgtatggtgttgggcctgatgaagacagaagatccaggcggcaacccgaccgagatattctgggggccccggatcgacatgagcaatccgttccatcccggccgccctctgcacggcaagtttgtgaccggtgaccagggcttgggccattgcatcgttcgccaaaccgacgtcgctgcggcccataagttctacagcctgctgggcttccgtggggacgtcgaataccgcattccattgcccaatggcatgactgccgaactgtcgttcatgcattgcaacgcccgtgatcactccattgcgtttggtgccatgcccgctgccaagcgcctcaatcacttgatgcttgagtacacccatatggaagacttgggatatacgcatcaacagttcgtgaagaacgaaattgacattgccttgcagcttggcattcacgccaacgacaaggcgctgacgttctacggcgcaacgccttcgggctggcttattgagcccggctggcgaggtgctacggccatagacgaagcggagtattacgtcggcgacatcttcggccatggcgtcgaggcacctggatatggcctggatgtaaaactgagttaaaagcgcttatgcgcacattgggtttctgacgttttctgcttcctatacagcacaataacaataacaataacaataacaactaagtggaatataataatgataaaaaaactgttccttacgtgctttgtttactgttcactcagcaccgcatgggccgaagaatcaccttggacataccgcatcggcatgagtaatattgcttttgatacaagcgccaaagtgtacttggatggccaccgtgtgccgggaggaagcgctgacgcgagcaataacaacgcgctcacattcgacttcggctacgccatcaacgaccagtggaatgcacgtttgattgtcggtattccgcctacaactaaagttaagggcgcaggcacacttccgggcattcagctggggaaaataacttatgctccaacaatattaacgttaaactataacctcccagcttttgggcccgttcgtcctcacatcggtgcgggagtcaattacacgcggattttggaaagcaaggacgctaatctaaaatcattcgatgccgaccacgcttggtcccccgcgctgcatgttggtgccgatattgacgtgagtcgcaactggttcgtcagcattgatatccggaagttatacctgaaaaccgacgcatcaggttacttagggccacaggaggctaaagcaaaggtaactcttgacccattgataacatcgatcgcgatcggacgccaattctgatgaccccttttaaggctctctatctatctaactgcaaagggtatttttatgttgaataaagttattaaaaccacgcgtcttaccgctgaagatatcaacggtgcctggactataatgcccacaccgtcgacgcctgatgcttctgattggcgcagcactaacactgtggacttagacgagactgcccgcatagttgaagagctgattgcagctggtgtcaacggtattttaagtatgggtacttttggtgagtgcgccacgttgacctgggaggagaaacgtgattatgtttcgacggttgtcgagaccattcgtggtcgtgtgccttatttctgtggcacgacggccctgaatacccgagaagtcatccgccagacccgagagcttatcgatattggcgctaacggcaccatgctaggcgtgccgatgtgggtgaagatggacctgcccacagcggtccagttctatcgtgatgttgcaggcgcggtaccggaggctgccattgcgatttacgccaaccccgaagcattcaaattcgacttccctcgcccattttgggcagagatgtccaaaattcctcaggtagtgactgccaagtatctaggcatcggaatgcttgacttggacctgaaattggcgcctaacatccgcttccttccacacgaggacgactattacgcggccgcacgcatcaatcccgagcgcataaccgcgttctggtcaagcggggccatgtgcggcccggctaccgctatcatgttgcgtgatgaagtggagcgggccaagagtaccggtgactggatcaaggccaaagccatctccgatgatatgcgtgcagccgattcgacattgtttccgcgtggcgacttttcggagttctcgaagtataacatcgggcttgaaaaggcacggatggacgcggctggttggctcaaggctggtccctgccgtcctccctacaatcttgttccagaagattacctcgttggtgcacagaaatcaggcaaggcgtgggccgcgctgcacgctaaatacagtaaataattaaagtagttcacctccgcaggcctgagcgcgagggttacgaacacgctgagcggtgcgggaagtaagtgagttaaagctcatctcttgcgccaggcactgctagatcagcaaagttagctgatctggcagtctcgaaaattttggcgaaagctgatcttaggaatgcgggataaaggcagtacaccgtaacgatgggggtgtgccgttcatgttgaacgacgccgctattgcgccgacttctcttcttcggagtgtttgattgtgattgtcgatttttatttcgattttttgagtccgttctcttacttggccaatcagcgcttgtcaaagcttgcgcaagattatggccttaccattcgttataacgcgatcgatttggcgcgggtcaaaatagccattggaaacgttggtccatccaatcgcgacttgaaagtcaaattggactatttgaaagtagatttgcaacggtgggcccagctttacggaataccgctggtattcccagctaactacaacagccgacggatgaatattgggttttattactcgggggccgaggcgcaggccgctgcctatgtgaatgtagtatttaatgcggtttggggagaaggcatagctccagatttggaaagcttgcctgccctggtatctgaaaagctaggctgggatcgtagtgctttcgagcactttctcagcagcaatgccgcaacagagaggtatgacgagcagacacatgccgccatcgagcgcaaggtgttcggtgtgccaacgatgtttttgggcgatgaaatgtggtgggggaacgaccgtctattcatgctcgagagcgcaatggggcgcttgtgccggcaaaatgccgatttaagtagttgatctgatcgttatttgctcgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z