LOCUS BBa_K1688022 1159 bp DNA linear 22-Nov-2024 DEFINITION Catechol 1,2 Dioxygenase + HlyA export tag ACCESSION BBa_K1688022 VERSION BBa_K1688022.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 Promoter CDS 39..971 /label=Dioxygenase misc_feature 39..41 /label=START misc_feature 974..1153 /label=HlyA C-tag misc_feature 1154..1156 /label=STOP misc_feature 1157..1159 /label=STOP misc_feature 972..973 /label=Frameshift insertion RBS 21..32 /label=RBS misc_feature 33..38 /label=3A Scar ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gaccgtgaaa attagccata 61 ccgcggatat tcaggcgttt tttaaccgcg tggcgggcct ggatcatgcg gaaggcaacc 121 cgcgctttaa acagattatt ctgcgcgtgc tacaggatac cgcgcgcctg attgaagatc 181 tggaaattac cgaagatgaa ttttggcatg cggtggatta tctgaaccgc ctgggtggcc 241 gcaacgaagc gggcctgctg gcggcgggcc tgggcattga acattttctg gatctgctac 301 aggatgcgaa agatgcggaa gcgggcctgg gcggcggcac cccgcgcacc attgaaggcc 361 cgctgtatgt ggcgggcgcg ccgctggcgc agggcgaagc gcgcatggat gatggcaccg 421 atccgggcgt ggtgatgttt ctacagggcc aggtgtttga tgcggatggc aaaccgctgg 481 cgggcgcgac cgtggatctg tggcatgcga acacccaggg cacctatagc tattttgata 541 gcacccagag cgaatttaac ctgcgccgcc gcattattac cgatgcggaa ggccgctatc 601 gcgcgcgcag cattgtgccg agcggctatg gctgcgatcc gcagggcccg acccaggaat 661 gcctggatct gctgggccgc catggccagc gcccggcgca tgtgcatttt tttattagcg 721 cgccgggcca tcgccatctg accacccaga ttaactttgc gggcgataaa tatctgtggg 781 atgattttgc gtatgcgacc cgcgatggcc tgattggcga actgcgcttt gtggaagatg 841 cggcggcggc gcgcgatcgc ggcgtgcagg gcgaacgctt tgcggaactg agctttgatt 901 ttcgcctaca gggcgcgaaa agcccggatg cggaagcgcg cagccatcgc ccgcgcgcgc 961 tacaggaagg cagttagcct atggaagtca gggtgatctt aatccattaa ttaatgaaat 1021 cagcaaaatc atttcagcag caggtagctt cgatgttaaa gaggaaagaa ccgcagcttc 1081 tttattgcag ttgtccggta atgccagtga tttttcatat ggacggaact caataaccct 1141 gaccacatca gcataataa //