LOCUS       BBa_K1688022            1159 bp    DNA     linear       14-Nov-2024
DEFINITION  Catechol 1,2 Dioxygenase + HlyA export tag
ACCESSION   BBa_K1688022
VERSION     BBa_K1688022.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 Promoter
     CDS             39..971
                     /label=Dioxygenase
     misc_feature    39..41
                     /label=START
     misc_feature    974..1153
                     /label=HlyA C-tag
     misc_feature    1154..1156
                     /label=STOP
     misc_feature    1157..1159
                     /label=STOP
     misc_feature    972..973
                     /label=Frameshift insertion 
     RBS             21..32
                     /label=RBS
     misc_feature    33..38
                     /label=3A Scar
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaccgtgaaa attagccata
       61 ccgcggatat tcaggcgttt tttaaccgcg tggcgggcct ggatcatgcg gaaggcaacc
      121 cgcgctttaa acagattatt ctgcgcgtgc tacaggatac cgcgcgcctg attgaagatc
      181 tggaaattac cgaagatgaa ttttggcatg cggtggatta tctgaaccgc ctgggtggcc
      241 gcaacgaagc gggcctgctg gcggcgggcc tgggcattga acattttctg gatctgctac
      301 aggatgcgaa agatgcggaa gcgggcctgg gcggcggcac cccgcgcacc attgaaggcc
      361 cgctgtatgt ggcgggcgcg ccgctggcgc agggcgaagc gcgcatggat gatggcaccg
      421 atccgggcgt ggtgatgttt ctacagggcc aggtgtttga tgcggatggc aaaccgctgg
      481 cgggcgcgac cgtggatctg tggcatgcga acacccaggg cacctatagc tattttgata
      541 gcacccagag cgaatttaac ctgcgccgcc gcattattac cgatgcggaa ggccgctatc
      601 gcgcgcgcag cattgtgccg agcggctatg gctgcgatcc gcagggcccg acccaggaat
      661 gcctggatct gctgggccgc catggccagc gcccggcgca tgtgcatttt tttattagcg
      721 cgccgggcca tcgccatctg accacccaga ttaactttgc gggcgataaa tatctgtggg
      781 atgattttgc gtatgcgacc cgcgatggcc tgattggcga actgcgcttt gtggaagatg
      841 cggcggcggc gcgcgatcgc ggcgtgcagg gcgaacgctt tgcggaactg agctttgatt
      901 ttcgcctaca gggcgcgaaa agcccggatg cggaagcgcg cagccatcgc ccgcgcgcgc
      961 tacaggaagg cagttagcct atggaagtca gggtgatctt aatccattaa ttaatgaaat
     1021 cagcaaaatc atttcagcag caggtagctt cgatgttaaa gaggaaagaa ccgcagcttc
     1081 tttattgcag ttgtccggta atgccagtga tttttcatat ggacggaact caataaccct
     1141 gaccacatca gcataataa
//