BBa_K1688022
1
Dioxy
Catechol 1,2 Dioxygenase + HlyA export tag
2015-09-15T11:00:00Z
2015-09-18T11:10:54Z
Restriction free cloning.
Catechol 1,2 dioxygenase (BBa_K1092003) fused to HlyA export tag (BBa_K554002), but with an introduced frameshift due to improper assembly.
false
false
_2106_
27118
27552
9
false
Primers for restriction-free cloning were designed in such a way as to remove the STOP codon of the catechol 1,2 dioxygenase and the scar left from 3A assembly. However, due to a design error, an AG insert between the two parts was introduced, resulting in a stop codon at the beginning of the HlyA sequence.
false
Delyan Georgiev
annotation2460971
1
START
range2460971
1
39
41
annotation2461088
1
3A Scar
range2461088
1
33
38
annotation2460969
1
T7 Promoter
range2460969
1
1
20
annotation2460970
1
Dioxygenase
range2460970
1
39
971
annotation2461022
1
Frameshift insertion
range2461022
1
972
973
annotation2461019
1
HlyA C-tag
range2461019
1
974
1153
annotation2461087
1
RBS
range2461087
1
21
32
annotation2461020
1
STOP
range2461020
1
1154
1156
annotation2461021
1
STOP
range2461021
1
1157
1159
BBa_K1688022_sequence
1
taatacgactcactatagggaaagaggagaaatactagatgaccgtgaaaattagccataccgcggatattcaggcgttttttaaccgcgtggcgggcctggatcatgcggaaggcaacccgcgctttaaacagattattctgcgcgtgctacaggataccgcgcgcctgattgaagatctggaaattaccgaagatgaattttggcatgcggtggattatctgaaccgcctgggtggccgcaacgaagcgggcctgctggcggcgggcctgggcattgaacattttctggatctgctacaggatgcgaaagatgcggaagcgggcctgggcggcggcaccccgcgcaccattgaaggcccgctgtatgtggcgggcgcgccgctggcgcagggcgaagcgcgcatggatgatggcaccgatccgggcgtggtgatgtttctacagggccaggtgtttgatgcggatggcaaaccgctggcgggcgcgaccgtggatctgtggcatgcgaacacccagggcacctatagctattttgatagcacccagagcgaatttaacctgcgccgccgcattattaccgatgcggaaggccgctatcgcgcgcgcagcattgtgccgagcggctatggctgcgatccgcagggcccgacccaggaatgcctggatctgctgggccgccatggccagcgcccggcgcatgtgcatttttttattagcgcgccgggccatcgccatctgaccacccagattaactttgcgggcgataaatatctgtgggatgattttgcgtatgcgacccgcgatggcctgattggcgaactgcgctttgtggaagatgcggcggcggcgcgcgatcgcggcgtgcagggcgaacgctttgcggaactgagctttgattttcgcctacagggcgcgaaaagcccggatgcggaagcgcgcagccatcgcccgcgcgcgctacaggaaggcagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z