BBa_K1688022 1 Dioxy Catechol 1,2 Dioxygenase + HlyA export tag 2015-09-15T11:00:00Z 2015-09-18T11:10:54Z Restriction free cloning. Catechol 1,2 dioxygenase (BBa_K1092003) fused to HlyA export tag (BBa_K554002), but with an introduced frameshift due to improper assembly. false false _2106_ 27118 27552 9 false Primers for restriction-free cloning were designed in such a way as to remove the STOP codon of the catechol 1,2 dioxygenase and the scar left from 3A assembly. However, due to a design error, an AG insert between the two parts was introduced, resulting in a stop codon at the beginning of the HlyA sequence. false Delyan Georgiev annotation2460971 1 START range2460971 1 39 41 annotation2461088 1 3A Scar range2461088 1 33 38 annotation2460969 1 T7 Promoter range2460969 1 1 20 annotation2460970 1 Dioxygenase range2460970 1 39 971 annotation2461022 1 Frameshift insertion range2461022 1 972 973 annotation2461019 1 HlyA C-tag range2461019 1 974 1153 annotation2461087 1 RBS range2461087 1 21 32 annotation2461020 1 STOP range2461020 1 1154 1156 annotation2461021 1 STOP range2461021 1 1157 1159 BBa_K1688022_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaccgtgaaaattagccataccgcggatattcaggcgttttttaaccgcgtggcgggcctggatcatgcggaaggcaacccgcgctttaaacagattattctgcgcgtgctacaggataccgcgcgcctgattgaagatctggaaattaccgaagatgaattttggcatgcggtggattatctgaaccgcctgggtggccgcaacgaagcgggcctgctggcggcgggcctgggcattgaacattttctggatctgctacaggatgcgaaagatgcggaagcgggcctgggcggcggcaccccgcgcaccattgaaggcccgctgtatgtggcgggcgcgccgctggcgcagggcgaagcgcgcatggatgatggcaccgatccgggcgtggtgatgtttctacagggccaggtgtttgatgcggatggcaaaccgctggcgggcgcgaccgtggatctgtggcatgcgaacacccagggcacctatagctattttgatagcacccagagcgaatttaacctgcgccgccgcattattaccgatgcggaaggccgctatcgcgcgcgcagcattgtgccgagcggctatggctgcgatccgcagggcccgacccaggaatgcctggatctgctgggccgccatggccagcgcccggcgcatgtgcatttttttattagcgcgccgggccatcgccatctgaccacccagattaactttgcgggcgataaatatctgtgggatgattttgcgtatgcgacccgcgatggcctgattggcgaactgcgctttgtggaagatgcggcggcggcgcgcgatcgcggcgtgcagggcgaacgctttgcggaactgagctttgattttcgcctacagggcgcgaaaagcccggatgcggaagcgcgcagccatcgcccgcgcgcgctacaggaaggcagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z