LOCUS       BBa_K1689000             557 bp    DNA     linear       22-Dec-2024
DEFINITION  sgRNA generator
ACCESSION   BBa_K1689000
VERSION     BBa_K1689000.1  
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label=T7 promoter
     CDS             27..302
                     /label=lacZa
     promoter        303..424
                     /label=lacZ promoter
     misc_feature    432..511
                     /label=crRNA
     misc_feature    512..557
                     /label=T7 terminator
ORIGIN
        1 taatacgact cactataggc gagaccccat tcgccattca ggctgcgcaa ctgttgggaa
       61 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
      121 aggcgattaa gttgggtaac gccagggttt tcccaatcac gacgttgtaa aacgacggcc
      181 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
      241 gtcctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
      301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      421 gcgcggtctc agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      481 cttgaaaaag tggcaccgag tcggtgcttt ttagcataac cccttggggc ctctaaacgg
      541 gtcttgaggg gtttttt
//