LOCUS       BBa_K1689003            1607 bp    DNA     linear       22-Dec-2024
DEFINITION  Coding sequence of Nluc416-FRB
ACCESSION   BBa_K1689003
VERSION     BBa_K1689003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23110
     RBS             36..47
                     /label=B0034
     CDS             51..1295
                     /label=N416 terminal domain of firefly luciferase
     CDS             1296..1325
                     /label=GGGGSGGGGS linker
     CDS             1326..1604
                     /label=FRB
     misc_feature    48..50
                     /label=start codon
     misc_feature    1605..1607
                     /label=stop codon
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagcaaaga ggagaaaatg gaagatgcga
       61 agaacatcaa gaaaggcccg gcaccgtttt atccgctgga agatggaaca gcaggtgaac
      121 agctgcataa agctatgaaa cgctatgcac tggttccggg aaccattgcg tttaccgatg
      181 cacatattga agtggatatt acctatgcag aatattttga aatgagcgtg cgtctggcag
      241 aagctatgaa acgctatggt ctgaatacca atcatcgtat tgttgtgtgt agcgaaaata
      301 gcctccagtt ctttatgccg gtgctgggcg cactgtttat tggcgtggca gtggcaccgg
      361 caaatgatat atataacgag cgcgaactgc tgaatagtat gggtatttca cagccgaccg
      421 ttgtgtttgt gtctaagaaa ggcttacaga agattctgaa tgttcagaag aaactgccga
      481 ttattcagaa aattattatt atggattcta aaaccgatta tcagggcttt cagtcaatgt
      541 atacctttgt gacctcacat ctgccgccgg gctttaacga gtatgatttt gttccggaat
      601 catttgatcg cgataaaacc attgcactga ttatgaatag ctcaggtagt accggcctgc
      661 cgaaaggcgt ggcactgccg catcgtaccg catgtgtgcg ctttagtcat gcacgcgatc
      721 cgatctttgg taatcagatt attccggata ccgcaattct gtcagtggtt ccgtttcatc
      781 atggctttgg catgtttacc accctgggct atctgatttg tggctttcgc gtggtgctga
      841 tgtatcgctt tgaagaagaa ctgtttctgc gctctcttca agattataag attcagagcg
      901 cactgctggt tccgaccctg tttagcttct tcgcaaaatc aaccctgatt gataaatatg
      961 atctgtctaa tctgcatgaa attgcaagcg gcggcgcacc gctgtctaaa gaagttggcg
     1021 aagcagtggc aaaacgcttt catctgccgg gcattcgcca gggttatggc ctgaccgaaa
     1081 ccacctcagc aattctgatt accccggaag gcgatgataa accgggcgca gtgggtaaag
     1141 tggttccgtt ctttgaagca aaagttgtgg atctggatac cggaaagacc ctgggcgtta
     1201 atcagcgcgg tgaactgtgt gttcgcggtc cgatgattat gtcaggctat gttaataacc
     1261 cggaagcaac caatgcactg attgataaag atggtggtgg tggtggtagc ggtggtggtg
     1321 gtagcatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact
     1381 ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg
     1441 aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa
     1501 tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcaccc
     1561 aagcctggga cctctattat catgtgttcc gacgaatctc atgataa
//