LOCUS       BBa_K1689004            1553 bp    DNA     linear       22-Dec-2024
DEFINITION  Coding sequence of Nluc398-FRB
ACCESSION   BBa_K1689004
VERSION     BBa_K1689004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23110
     RBS             36..47
                     /label=B0034
     CDS             51..1240
                     /label=N398 terminal of firefly luciferase
     CDS             1242..1271
                     /label=GGGGSGGGGS linker
     CDS             1272..1550
                     /label=FRB
     misc_feature    48..50
                     /label=start codon
     misc_feature    1551..1553
                     /label=stop codon
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagcaaaga ggagaaaatg gaagatgcga
       61 agaacatcaa gaaaggcccg gcaccgtttt atccgctgga agatggaaca gcaggtgaac
      121 agctgcataa agctatgaaa cgctatgcac tggttccggg aaccattgcg tttaccgatg
      181 cacatattga agtggatatt acctatgcag aatattttga aatgagcgtg cgtctggcag
      241 aagctatgaa acgctatggt ctgaatacca atcatcgtat tgttgtgtgt agcgaaaata
      301 gcctccagtt ctttatgccg gtgctgggcg cactgtttat tggcgtggca gtggcaccgg
      361 caaatgatat atataacgag cgcgaactgc tgaatagtat gggtatttca cagccgaccg
      421 ttgtgtttgt gtctaagaaa ggcttacaga agattctgaa tgttcagaag aaactgccga
      481 ttattcagaa aattattatt atggattcta aaaccgatta tcagggcttt cagtcaatgt
      541 atacctttgt gacctcacat ctgccgccgg gctttaacga gtatgatttt gttccggaat
      601 catttgatcg cgataaaacc attgcactga ttatgaatag ctcaggtagt accggcctgc
      661 cgaaaggcgt ggcactgccg catcgtaccg catgtgtgcg ctttagtcat gcacgcgatc
      721 cgatctttgg taatcagatt attccggata ccgcaattct gtcagtggtt ccgtttcatc
      781 atggctttgg catgtttacc accctgggct atctgatttg tggctttcgc gtggtgctga
      841 tgtatcgctt tgaagaagaa ctgtttctgc gctctcttca agattataag attcagagcg
      901 cactgctggt tccgaccctg tttagcttct tcgcaaaatc aaccctgatt gataaatatg
      961 atctgtctaa tctgcatgaa attgcaagcg gcggcgcacc gctgtctaaa gaagttggcg
     1021 aagcagtggc aaaacgcttt catctgccgg gcattcgcca gggttatggc ctgaccgaaa
     1081 ccacctcagc aattctgatt accccggaag gcgatgataa accgggcgca gtgggtaaag
     1141 tggttccgtt ctttgaagca aaagttgtgg atctggatac cggaaagacc ctgggcgtta
     1201 atcagcgcgg tgaactgtgt gttcgcggtc cgatgattat gggtggtggt ggtagcggtg
     1261 gtggtggtag catcctctgg catgagatgt ggcatgaagg cctggaagag gcatctcgtt
     1321 tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta
     1381 tgatggaacg gggcccccag actctgaagg aaacatcctt taatcaggcc tatggtcgag
     1441 atttaatgga ggcccaagag tggtgcagga agtacatgaa atcagggaat gtcaaggacc
     1501 tcacccaagc ctgggacctc tattatcatg tgttccgacg aatctcatga taa
//