LOCUS BBa_K1689008 5630 bp DNA linear 14-Nov-2024 DEFINITION dCas9-N-luc ACCESSION BBa_K1689008 VERSION BBa_K1689008.1 FEATURES Location/Qualifiers RBS 60..77 /label=B0034 misc_feature 78..80 /label=start codon CDS 81..4181 /label=dCas9 CDS 4182..4211 /label=GGGGSGGGGS linker CDS 4212..5456 /label=NFluc416 misc_feature 5457..5459 /label=stop codon misc_feature 5475..5553 /label=rnnB T1 terminator misc_feature 1..59 /label=random spacer misc_feature 5554..5630 /label=random spacer ORIGIN 1 tgggatccgc ggaggatatg gctgccgacg aaacagcctc tacaaataat tttgtttaaa 61 aagaggagaa atactagatg gataagaaat actcaatagg cttagctatc ggcacaaata 121 gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag ttcaaggttc 181 tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt ttatttgaca 241 gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg tatacacgtc 301 ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg aaagtagatg 361 atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag aagcatgaac 421 gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa tatccaacta 481 tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg cgcttaatct 541 atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag ggagatttaa 601 atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc tacaatcaat 661 tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt ctttctgcac 721 gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt gagaagaaaa 781 atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat tttaaatcaa 841 attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac gatgatgatt 901 tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg gcagctaaga 961 atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa ataactaagg 1021 ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac ttgactcttt 1081 taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt tttgatcaat 1141 caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa ttttataaat 1201 ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg aaactaaatc 1261 gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc catcaaattc 1321 acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca tttttaaaag 1381 acaatcgtga gaagattgaa aaaatcttga cttttcgact tccttattat gttggtccat 1441 tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa acaattaccc 1501 catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt attgaacgca 1561 tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat agtttgcttt 1621 atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact gaaggaatgc 1681 gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta ctcttcaaaa 1741 caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa atagaatgtt 1801 ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc 1861 atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata 1921 tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa 1981 gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc 2041 gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat 2101 ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc 2161 agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg 2221 gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa 2281 aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata 2341 agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga 2401 aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga 2461 ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt 2521 atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg 2581 attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca atagacaata 2641 aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag 2701 tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac 2761 gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg 2821 gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt 2881 tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag 2941 tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag 3001 tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa 3061 ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag 3121 tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa 3181 aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg 3241 gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg 3301 ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg 3361 tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta ccaaaaagaa 3421 attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg 3481 atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga 3541 agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga agttcctttg 3601 aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa aaagacttaa 3661 tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa cggatgctgg 3721 ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa tatgtgaatt 3781 ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat aacgaacaaa 3841 aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag caaatcagtg 3901 aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt agtgcatata 3961 acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat ttatttacgt 4021 tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt gatcgtaaac 4081 gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc atcactggtc 4141 tttatgaaac acgcattgat ttgagtcagc taggaggtga cggtggcggt ggctctggtg 4201 gcggtggctc tgaagatgcg aagaacatca agaaaggccc ggcaccgttt tatccgctgg 4261 aagatggaac agcaggtgaa cagctgcata aagctatgaa acgctatgca ctggttccgg 4321 gaaccattgc gtttaccgat gcacatattg aagtggatat tacctatgca gaatattttg 4381 aaatgagcgt gcgtctggca gaagctatga aacgctatgg tctgaatacc aatcatcgta 4441 ttgttgtgtg tagcgaaaat agcctccagt tctttatgcc ggtgctgggc gcactgttta 4501 ttggcgtggc agtggcaccg gcaaatgata tatataacga gcgcgaactg ctgaatagta 4561 tgggtatttc acagccgacc gttgtgtttg tgtctaagaa aggcttacag aagattctga 4621 atgttcagaa gaaactgccg attattcaga aaattattat tatggattct aaaaccgatt 4681 atcagggctt tcagtcaatg tatacctttg tgacctcaca tctgccgccg ggctttaacg 4741 agtatgattt tgttccggaa tcatttgatc gcgataaaac cattgcactg attatgaata 4801 gctcaggtag taccggcctg ccgaaaggcg tggcactgcc gcatcgtacc gcatgtgtgc 4861 gctttagtca tgcacgcgat ccgatctttg gtaatcagat tattccggat accgcaattc 4921 tgtcagtggt tccgtttcat catggctttg gcatgtttac caccctgggc tatctgattt 4981 gtggctttcg cgtggtgctg atgtatcgct ttgaagaaga actgtttctg cgctctcttc 5041 aagattataa gattcagagc gcactgctgg ttccgaccct gtttagcttc ttcgcaaaat 5101 caaccctgat tgataaatat gatctgtcta atctgcatga aattgcaagc ggcggcgcac 5161 cgctgtctaa agaagttggc gaagcagtgg caaaacgctt tcatctgccg ggcattcgcc 5221 agggttatgg cctgaccgaa accacctcag caattctgat taccccggaa ggcgatgata 5281 aaccgggcgc agtgggtaaa gtggttccgt tctttgaagc aaaagttgtg gatctggata 5341 ccggaaagac cctgggcgtt aatcagcgcg gtgaactgtg tgttcgcggt ccgatgatta 5401 tgtcaggcta tgttaataac ccggaagcaa ccaatgcact gattgataaa gatggttaat 5461 actagagtaa tagtccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 5521 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagg 5581 tgatatcagt gttccacaaa ctgatgtcaa gcgtgtacga atttgataat //