LOCUS BBa_K1689009 4841 bp DNA linear 14-Nov-2024 DEFINITION C-luc-dCas9 ACCESSION BBa_K1689009 VERSION BBa_K1689009.1 FEATURES Location/Qualifiers RBS 60..77 /label=B0034 misc_feature 78..80 /label=start codon CDS 81..536 /label=CFluc398 CDS 537..566 /label=GGGGSGGGGS linker CDS 567..4667 /label=dCas9 misc_feature 4668..4670 /label=stop codon misc_feature 4686..4764 /label=rrnB T1 terminator misc_feature 1..59 /label=random spacer misc_feature 4765..4841 /label=random spacer ORIGIN 1 tgggatccgc ggaggatatg gctgccgacg aaacagcctc tacaaataat tttgtttaaa 61 aagaggagaa atactagatg tcaggctatg ttaataaccc ggaagcaacc aatgcactga 121 ttgataaaga tggttggctg catagcggcg atattgcata ttgggatgaa gatgaacact 181 tcttcattgt ggatcgtctg aaatctctga ttaaatacaa aggctatcag gttgcaccgg 241 cagaactgga atcaattctg cttcagcatc cgaacatctt tgatgcaggt gtggcaggcc 301 tgccggatga tgatgcaggt gaactgccgg cagcagttgt ggtgctggaa catgggaaaa 361 ccatgaccga gaaagaaatt gttgattatg ttgcaagcca ggtgaccacc gcaaagaaac 421 tgcgcggcgg cgtggtgttt gtggatgaag ttccgaaagg cctgaccggt aaactggatg 481 cacgcaaaat tcgcgaaatt ctgattaaag cgaagaaagg cggcaagatt gcagtgggtg 541 gcggtggctc tggtggcggt ggctctgata agaaatactc aataggctta gctatcggca 601 caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca 661 aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat 721 ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata 781 cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag 841 tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc 901 atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc 961 caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct 1021 taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag 1081 atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca 1141 atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt 1201 ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga 1261 agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta 1321 aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg 1381 atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag 1441 ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa 1501 ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga 1561 ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg 1621 atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt 1681 ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac 1741 taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc 1801 aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt 1861 taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgacttcct tattatgttg 1921 gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa 1981 ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg 2041 aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt 2101 tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag 2161 gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct 2221 tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag 2281 aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta 2341 cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg 2401 aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg 2461 aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac 2521 gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata 2581 agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt 2641 ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag 2701 tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta 2761 ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc 2821 ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg 2881 gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa 2941 gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc 3001 tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt 3061 taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac gattcaatag 3121 acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg 3181 aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca 3241 ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata 3301 aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac 3361 aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg 3421 ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct 3481 ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg 3541 ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt 3601 ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa 3661 ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg 3721 caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg 3781 tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca 3841 atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa 3901 aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg 3961 gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga 4021 aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt 4081 cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag 4141 acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga 4201 tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg 4261 tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg 4321 aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa 4381 tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg 4441 catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat 4501 ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc 4561 gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca 4621 ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactaa tactagagta 4681 atagtccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 4741 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag gtgatatcag 4801 tgttccacaa actgatgtca agcgtgtacg aatttgataa t //