LOCUS BBa_K1689010 5630 bp DNA linear 14-Nov-2024 DEFINITION N-luc-dCas9 ACCESSION BBa_K1689010 VERSION BBa_K1689010.1 FEATURES Location/Qualifiers misc_feature 1..59 /label=random spacer RBS 60..77 /label=B0034 misc_feature 78..80 /label=start codon CDS 81..1325 /label=NFluc416 CDS 1326..1355 /label=GGGGSGGGGS linker CDS 1356..5456 /label=dCas9 misc_feature 5457..5459 /label=stop codon misc_feature 5475..5553 /label=rrnB T1 terminator misc_feature 5554..5630 /label=random spacer ORIGIN 1 tgggatccgc ggaggatatg gctgccgacg aaacagcctc tacaaataat tttgtttaaa 61 aagaggagaa atactagatg gaagatgcga agaacatcaa gaaaggcccg gcaccgtttt 121 atccgctgga agatggaaca gcaggtgaac agctgcataa agctatgaaa cgctatgcac 181 tggttccggg aaccattgcg tttaccgatg cacatattga agtggatatt acctatgcag 241 aatattttga aatgagcgtg cgtctggcag aagctatgaa acgctatggt ctgaatacca 301 atcatcgtat tgttgtgtgt agcgaaaata gcctccagtt ctttatgccg gtgctgggcg 361 cactgtttat tggcgtggca gtggcaccgg caaatgatat atataacgag cgcgaactgc 421 tgaatagtat gggtatttca cagccgaccg ttgtgtttgt gtctaagaaa ggcttacaga 481 agattctgaa tgttcagaag aaactgccga ttattcagaa aattattatt atggattcta 541 aaaccgatta tcagggcttt cagtcaatgt atacctttgt gacctcacat ctgccgccgg 601 gctttaacga gtatgatttt gttccggaat catttgatcg cgataaaacc attgcactga 661 ttatgaatag ctcaggtagt accggcctgc cgaaaggcgt ggcactgccg catcgtaccg 721 catgtgtgcg ctttagtcat gcacgcgatc cgatctttgg taatcagatt attccggata 781 ccgcaattct gtcagtggtt ccgtttcatc atggctttgg catgtttacc accctgggct 841 atctgatttg tggctttcgc gtggtgctga tgtatcgctt tgaagaagaa ctgtttctgc 901 gctctcttca agattataag attcagagcg cactgctggt tccgaccctg tttagcttct 961 tcgcaaaatc aaccctgatt gataaatatg atctgtctaa tctgcatgaa attgcaagcg 1021 gcggcgcacc gctgtctaaa gaagttggcg aagcagtggc aaaacgcttt catctgccgg 1081 gcattcgcca gggttatggc ctgaccgaaa ccacctcagc aattctgatt accccggaag 1141 gcgatgataa accgggcgca gtgggtaaag tggttccgtt ctttgaagca aaagttgtgg 1201 atctggatac cggaaagacc ctgggcgtta atcagcgcgg tgaactgtgt gttcgcggtc 1261 cgatgattat gtcaggctat gttaataacc cggaagcaac caatgcactg attgataaag 1321 atggtggtgg cggtggctct ggtggcggtg gctctgataa gaaatactca ataggcttag 1381 ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta 1441 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg 1501 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta 1561 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga 1621 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag 1681 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg 1741 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg 1801 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga 1861 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac 1921 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag 1981 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc 2041 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc 2101 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata 2161 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt 2221 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata 2281 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc 2341 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa 2401 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag 2461 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat 2521 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta 2581 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt 2641 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgacttcctt 2701 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg 2761 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat 2821 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa 2881 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg 2941 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg 3001 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca 3061 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt 3121 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg 3181 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg 3241 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac 3301 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta 3361 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca 3421 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa 3481 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta 3541 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag 3601 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa 3661 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag 3721 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa 3781 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata 3841 ttaatcgttt aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg 3901 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg 3961 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca 4021 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg 4081 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc 4141 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta 4201 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt 4261 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa 4321 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct 4381 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag 4441 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa 4501 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg 4561 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc 4621 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa 4681 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa 4741 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg 4801 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg 4861 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag 4921 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc 4981 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa 5041 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag 5101 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta 5161 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag 5221 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta 5281 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa 5341 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc 5401 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat 5461 actagagtaa tagtccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 5521 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagg 5581 tgatatcagt gttccacaaa ctgatgtcaa gcgtgtacga atttgataat //