LOCUS BBa_K1689011 7235 bp DNA linear 22-Nov-2024 DEFINITION dCas9-delta alpha ACCESSION BBa_K1689011 VERSION BBa_K1689011.1 FEATURES Location/Qualifiers CDS 34..7164 /label=dCas9-delta alpha misc_feature 37..54 /label=His tag CDS 4156..4185 /label=GGGGS*2 Linker CDS 55..4155 /label=dCas9 CDS 4186..7161 /label=delta alpha misc_feature 1..33 /label=random spacer misc_feature 34..37 /label=start misc_feature 7162..7164 /label=stop misc_feature 7165..7235 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacgataag 61 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat 121 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc 181 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt 241 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag 301 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag 361 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta 421 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta 481 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag 541 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa 601 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca 661 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa 721 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct 781 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa 841 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga 901 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca 961 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa 1021 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt 1081 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt 1141 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg 1201 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg 1261 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg 1321 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc 1381 ttgacttttc gcattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca 1441 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat 1501 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca 1561 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa 1621 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa 1681 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa 1741 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt 1801 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat 1861 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg 1921 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt 1981 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct 2041 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg 2101 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca 2161 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat 2221 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt 2281 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg 2341 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga 2401 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat 2461 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat 2521 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca 2581 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat 2641 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg 2701 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct 2761 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa 2821 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac 2881 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt 2941 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat 3001 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa 3061 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct 3121 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg 3181 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc 3241 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg 3301 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc 3361 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa 3421 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc 3481 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta 3541 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa 3601 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt 3661 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga 3721 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa 3781 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag 3841 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca 3901 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt 3961 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct 4021 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta 4081 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt 4141 cagctaggag gtgacggtgg cggtggctct ggtggcggtg gctctaccat gattacggat 4201 tcactggccg tcgaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 4261 gaatggcgct ttgcctggtt tccggtacca gaagcggtgc cggaaagctg gctggagtgc 4321 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat 4381 gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg 4441 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa 4501 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg 4561 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt 4621 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat 4681 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat 4741 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc 4801 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta 4861 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt 4921 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa 4981 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac 5041 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg 5101 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt 5161 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc 5221 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg 5281 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa 5341 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg 5401 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg 5461 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa 5521 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc 5581 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg 5641 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc 5701 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt 5761 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt 5821 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg 5881 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg 5941 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc 6001 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg 6061 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt 6121 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc 6181 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc 6241 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc 6301 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg 6361 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg 6421 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt 6481 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac 6541 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt 6601 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac 6661 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca 6721 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac 6781 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac 6841 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt 6901 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag 6961 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg 7021 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac 7081 gactcctgga gcccgtcagt atcggcggaa tttcagctga gcgccggtcg ctaccattac 7141 cagttggtct ggtgtcaaaa atgagatccg gctgctaaca aggaggtgat atcagtgttc 7201 cacaaactga tgtcaagcgt gtacgaattt gataa //