LOCUS BBa_K1689012 4772 bp DNA linear 14-Nov-2024 DEFINITION dCas9-Nlact ACCESSION BBa_K1689012 VERSION BBa_K1689012.1 FEATURES Location/Qualifiers misc_feature 37..54 /label=His CDS 34..4701 /label=dCas9-Nlact CDS 55..4155 /label=dCas9 CDS 4156..4185 /label=GGGGS*2 Linker CDS 4186..4698 /label=Nlact for N terminal of β-Lactamase misc_feature 34..36 /label=start misc_feature 1..33 /label=random spacer misc_feature 4699..4701 /label=stop misc_feature 4702..4772 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacgataag 61 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat 121 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc 181 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt 241 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag 301 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag 361 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta 421 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta 481 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag 541 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa 601 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca 661 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa 721 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct 781 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa 841 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga 901 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca 961 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa 1021 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt 1081 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt 1141 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg 1201 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg 1261 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg 1321 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc 1381 ttgacttttc gcattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca 1441 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat 1501 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca 1561 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa 1621 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa 1681 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa 1741 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt 1801 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat 1861 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg 1921 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt 1981 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct 2041 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg 2101 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca 2161 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat 2221 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt 2281 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg 2341 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga 2401 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat 2461 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat 2521 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca 2581 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat 2641 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg 2701 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct 2761 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa 2821 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac 2881 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt 2941 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat 3001 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa 3061 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct 3121 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg 3181 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc 3241 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg 3301 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc 3361 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa 3421 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc 3481 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta 3541 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa 3601 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt 3661 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga 3721 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa 3781 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag 3841 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca 3901 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt 3961 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct 4021 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta 4081 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt 4141 cagctaggag gtgacggtgg cggtggctct ggtggcggtg gctctcaccc agaaacgctg 4201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttatat cgaactggat 4261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 4321 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 4381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 4441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 4501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 4561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 4621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 4681 cgcaaactat taactggctg agatccggct gctaacaagg aggtgatatc agtgttccac 4741 aaactgatgt caagcgtgta cgaatttgat aa //