LOCUS BBa_K1689013 4772 bp DNA linear 14-Nov-2024 DEFINITION Nlact-dCas9 ACCESSION BBa_K1689013 VERSION BBa_K1689013.1 FEATURES Location/Qualifiers CDS 34..4701 /label=Nlact-dCas9 misc_feature 37..54 /label=His CDS 55..567 /label=Nlact for N terminal of β-Lactamase CDS 568..697 /label=GGGGS*2 Linker CDS 598..4698 /label=dCas9 misc_feature 34..36 /label=start misc_feature 1..33 /label=random spacer misc_feature 4701..4772 /label=random spacer misc_feature 4699..4701 /label=stop ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcaccaccca 61 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttatatc 121 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 181 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 241 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 301 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 361 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 421 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 481 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 541 acaacgttgc gcaaactatt aactggcggt ggcggtggct ctggtggcgg tggctctgat 601 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact 661 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt 721 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact 781 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta 841 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa 901 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata 961 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg 1021 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt 1081 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac 1141 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac 1201 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta 1261 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt 1321 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct 1381 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt 1441 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt 1501 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt 1561 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa 1621 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat 1681 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa 1741 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa 1801 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt 1861 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa 1921 atcttgactt ttcgcattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt 1981 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc 2041 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt 2101 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac 2161 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt 2221 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag 2281 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga 2341 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa 2401 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca 2461 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc 2521 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg 2581 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt 2641 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg 2701 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa 2761 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa 2821 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa 2881 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa 2941 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa 3001 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg 3061 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt 3121 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa 3181 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat 3241 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa 3301 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt 3361 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa 3421 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta 3481 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat 3541 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca 3601 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt 3661 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc 3721 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta 3781 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca 3841 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca 3901 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt 3961 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca 4021 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag 4081 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta 4141 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt 4201 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa 4261 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat 4321 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat 4381 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta 4441 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata 4501 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct 4561 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt 4621 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg 4681 agtcagctag gaggtgactg agatccggct gctaacaagg aggtgatatc agtgttccac 4741 aaactgatgt caagcgtgta cgaatttgat aa //