LOCUS BBa_K1689015 4520 bp DNA linear 22-Nov-2024 DEFINITION F[1,2]-dCas9 ACCESSION BBa_K1689015 VERSION BBa_K1689015.1 FEATURES Location/Qualifiers misc_feature 37..54 /label=His CDS 55..315 /label=F[1,2] CDS 346..4449 /label=dCas9 CDS 316..345 /label=GGGGS*2 Linker CDS 34..4452 /label=F[1,2]-dCas9 misc_feature 1..33 /label=random spacer misc_feature 34..36 /label=start misc_feature 4450..4452 /label=stop misc_feature 4453..4520 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacatcagt 61 ctgattgcgg cgttagcggt agatcgcgtt atcggcatgg aaaacgccat gccgtggaac 121 ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa ataaacccgt gattatgggc 181 cgccatacct gggaatcaat cggtcgtccg ttgccaggac gcaaaaatat tatcctcagc 241 agtcaaccgg gtacggacga tcgcgtaacg tgggtgaagt cggtggatga agccatcgcg 301 gcgtgtggtg acgtaggtgg cggtggctct ggtggcggtg gctctgataa gaaatactca 361 ataggcttag ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag 421 gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat 481 cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg 541 acagctcgta gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt 601 tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg 661 gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt 721 gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact 781 gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt 841 cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc 901 cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta 961 gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt 1021 gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg 1081 ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt 1141 tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat 1201 gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta 1261 agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat 1321 gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag 1381 tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga 1441 gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact 1501 gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac 1561 aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa 1621 gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt 1681 cgcattcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact 1741 cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct 1801 tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa 1861 gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga attgacaaag 1921 gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa 1981 gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa 2041 gattatttca aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga 2101 tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt 2161 ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt 2221 gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag 2281 gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg 2341 attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat 2401 ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa 2461 gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat 2521 ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa 2581 ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa 2641 aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa 2701 ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg 2761 caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa 2821 gaattagata ttaatcgttt aagtgattat gatgtcgatg ccattgttcc acaaagtttc 2881 cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa 2941 tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt 3001 ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga 3061 ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa 3121 atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat 3181 gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc 3241 cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat 3301 gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg 3361 gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag 3421 caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc 3481 aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat 3541 ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta 3601 ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc 3661 aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg 3721 gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt 3781 gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc 3841 acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga 3901 tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta 3961 gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg 4021 gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag 4081 ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta 4141 gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat 4201 ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca 4261 gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat 4321 tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact 4381 cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga 4441 ggtgactgag atccggctgc taacaaggag gtgatatcag tgttccacaa actgatgtca 4501 agcgtgtacg aatttgataa //