LOCUS BBa_K1689017 4472 bp DNA linear 22-Dec-2024 DEFINITION F3-dCas9 ACCESSION BBa_K1689017 VERSION BBa_K1689017.1 FEATURES Location/Qualifiers CDS 34..4401 /label=F3-dCas9 misc_feature 37..54 /label=His tag CDS 55..267 /label=F3 CDS 268..297 /label=GGGGS*2 Linker CDS 298..4398 /label=dCas9 misc_feature 1..33 /label=random spacer misc_feature 34..36 /label=start misc_feature 4398..4401 /label=stop misc_feature 4402..4472 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacccagaa 61 atcatggtga ttggcggcgg tcgcgtttat gaacagttct tgccaaaagc gcaaaaactg 121 tatctgacgc atatcgacgc agaagtggaa ggcgacaccc atttcccgga ttacgagccg 181 gatgactggg aatcggtatt cagcgagttc cacgatgctg atgcgcagaa ctctcacagc 241 tattgctttg agattctgga gcggcggggt ggcggtggct ctggtggcgg tggctctgat 301 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact 361 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt 421 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact 481 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta 541 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa 601 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata 661 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg 721 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt 781 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac 841 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac 901 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta 961 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt 1021 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct 1081 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt 1141 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt 1201 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt 1261 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa 1321 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat 1381 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa 1441 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa 1501 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt 1561 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa 1621 atcttgactt ttcgcattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt 1681 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc 1741 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt 1801 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac 1861 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt 1921 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag 1981 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga 2041 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa 2101 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca 2161 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc 2221 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg 2281 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt 2341 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg 2401 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa 2461 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa 2521 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa 2581 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa 2641 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa 2701 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg 2761 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt 2821 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa 2881 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat 2941 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa 3001 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt 3061 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa 3121 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta 3181 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat 3241 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca 3301 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt 3361 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc 3421 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta 3481 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca 3541 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca 3601 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt 3661 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca 3721 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag 3781 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta 3841 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt 3901 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa 3961 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat 4021 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat 4081 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta 4141 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata 4201 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct 4261 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt 4321 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg 4381 agtcagctag gaggtgactg agatccggct gctaacaagg aggtgatatc agtgttccac 4441 aaactgatgt caagcgtgta cgaatttgat aa //