LOCUS BBa_K1689019 7235 bp DNA linear 27-Dec-2024 DEFINITION delta alpha-dCas9 ACCESSION BBa_K1689019 VERSION BBa_K1689019.1 FEATURES Location/Qualifiers misc_feature 34..36 /label=start codon CDS 34..7164 /label=delta alpha-dCas9 misc_feature 37..54 /label=6*His CDS 55..3030 /label=delta alpha CDS 3031..3060 /label=GGGGS*2 Linker CDS 3061..7161 /label=dCas9 misc_feature 7162..7164 /label=stop codon misc_feature 1..33 /label=random spacer misc_feature 7165..7235 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacaccatg 61 attacggatt cactggccgt cgaggcccgc accgatcgcc cttcccaaca gttgcgcagc 121 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 181 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 241 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 301 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 361 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 421 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 481 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 541 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 601 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 661 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 721 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 781 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 841 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 901 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 961 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1021 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1081 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1141 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1201 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1261 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1321 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1381 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1441 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1501 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1561 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1621 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1681 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1741 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1801 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1861 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 1921 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 1981 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2041 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2101 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2161 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2221 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2281 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2341 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2401 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2461 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2521 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2581 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2641 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2701 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2761 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2821 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 2881 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 2941 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc 3001 taccattacc agttggtctg gtgtcaaaaa ggtggcggtg gctctggtgg cggtggctct 3061 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc 3121 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac 3181 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg 3241 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat 3301 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt 3361 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat 3421 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa 3481 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg 3541 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg 3601 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt 3661 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga 3721 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc 3781 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat 3841 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa 3901 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta 3961 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg 4021 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa 4081 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt 4141 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa 4201 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag 4261 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct 4321 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa 4381 aaaatcttga cttttcgcat tccttattat gttggtccat tggcgcgtgg caatagtcgt 4441 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt 4501 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat 4561 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat 4621 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca 4681 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt 4741 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca 4801 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt 4861 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta 4921 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac 4981 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt 5041 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat 5101 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt 5161 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat 5221 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta 5281 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt 5341 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg 5401 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt 5461 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac 5521 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt 5581 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat 5641 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac 5701 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg 5761 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg 5821 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact 5881 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa 5941 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac 6001 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat 6061 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg 6121 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat 6181 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct 6241 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc 6301 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag 6361 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct 6421 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat 6481 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa 6541 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt 6601 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat 6661 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa 6721 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat 6781 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag 6841 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt 6901 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca 6961 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc 7021 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa 7081 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat 7141 ttgagtcagc taggaggtga ctgagatccg gctgctaaca aggaggtgat atcagtgttc 7201 cacaaactga tgtcaagcgt gtacgaattt gataa //