LOCUS BBa_K1689020 6647 bp DNA linear 09-Jan-2025 DEFINITION dCas9-delta omega ACCESSION BBa_K1689020 VERSION BBa_K1689020.1 FEATURES Location/Qualifiers misc_feature 34..36 /label=start codon CDS 34..6576 /label=delta omega-dCas9 misc_feature 37..54 /label=6*His CDS 55..4155 /label=dCas9 CDS 4156..4185 /label=GGGGS*2 Linker CDS 4186..6573 /label=lacz delta omega 11-797 misc_feature 6574..6576 /label=stop codon misc_feature 1..33 /label=random spacer misc_feature 6577..6647 /label=random spacer ORIGIN 1 atgggatccg cggaggatat ggctgccgac gaaatgcatc atcatcatca tcacgataag 61 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat 121 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc 181 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt 241 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag 301 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag 361 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta 421 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta 481 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag 541 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa 601 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca 661 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa 721 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct 781 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa 841 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga 901 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca 961 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa 1021 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt 1081 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt 1141 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg 1201 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg 1261 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg 1321 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc 1381 ttgacttttc gcattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca 1441 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat 1501 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca 1561 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa 1621 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa 1681 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa 1741 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt 1801 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat 1861 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg 1921 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt 1981 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct 2041 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg 2101 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca 2161 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat 2221 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt 2281 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg 2341 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga 2401 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat 2461 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat 2521 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca 2581 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat 2641 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg 2701 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct 2761 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa 2821 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac 2881 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt 2941 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat 3001 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa 3061 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct 3121 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg 3181 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc 3241 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg 3301 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc 3361 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa 3421 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc 3481 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta 3541 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa 3601 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt 3661 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga 3721 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa 3781 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag 3841 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca 3901 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt 3961 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct 4021 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta 4081 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt 4141 cagctaggag gtgacggtgg cggtggctct ggtggcggtg gctctaccat gattacggat 4201 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 4261 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 4321 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca 4381 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc 4441 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat 4501 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc 4561 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc 4621 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt 4681 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg 4741 gtgatggtgc tgcggtggag tgacggcagt tatctggaag atcaggatat gtggcggatg 4801 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc 4861 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag 4921 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg 4981 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat 5041 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc 5101 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca 5161 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac 5221 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag 5281 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac 5341 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac 5401 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt 5461 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag 5521 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc 5581 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag 5641 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc 5701 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg 5761 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt 5821 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc 5881 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg 5941 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt 6001 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag 6061 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt 6121 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc 6181 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta 6241 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg 6301 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac 6361 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg 6421 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt 6481 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc 6541 cgtgcaccgc tggataacga cattggcgta agttgagatc cggctgctaa caaggaggtg 6601 atatcagtgt tccacaaact gatgtcaagc gtgtacgaat ttgataa //