LOCUS BBa_K1692021 5176 bp DNA linear 26-Nov-2024 DEFINITION PanK + hybrid promoter phaCAB ACCESSION BBa_K1692021 VERSION BBa_K1692021.1 FEATURES Location/Qualifiers CDS 1..897 /label=BBa_K1692020 promoter 906..940 /label=BBa_J23104 misc_feature 953..956 /label=conserved RBS 949..960 /label=BBa_B0034 CDS 1321..3090 /label=phaC1 CDS 3175..4356 /label=phaA CDS 4431..5171 /label=phaB1 promoter 969..1018 /label=Pwt RBS 1304..1310 /label=RBS RBS 3164..3167 /label=RBS RBS 4421..4424 /label=RBS misc_feature 969..5176 /label=BBa_K934001 misc_feature 906..5176 /label=BBa_K1149051 ORIGIN 1 attgacagct agctcagtcc taggtattgt gctagctact agagtcacac aggaaagtac 61 tagatgaaag tcggtatcga tgctggtggc accctgatta agatcgtgca ggaacaggat 121 aaccagcgta cctttaaaac tgaactgacc aagaacatcg accaggtcgt ggaatggctg 181 aaccagcagc agattgaaaa gctgtgcctg accggcggca acgctggtgt tatcgccgaa 241 aacatcaaca ttccggcgca aatctttgtg gagttcgatg cggcttccca gggtctgggc 301 attctgctga aagaacaagg tcatgacctg gccgactaca tcttcgcaaa cgtcggcact 361 ggcaccagcc tgcactactt tgacggccag agccagcgcc gcgttggtgg tatcggcacc 421 ggtggcggta tgatccaggg cctgggttac ctgctgtctc agattaccga ttacaaacag 481 ctgactgaca tggcgcagca cggtgaccgt aacacgattg atctgaaagt gcgtcacatc 541 tataaagata ccgaaccgcc gatcccaggc gacctgactg ctgcgaactt tggtcatgtg 601 ctgcatcacc tggatgccga cttcacccct tccaacaaac tggcagcggt tattggcgtt 661 gtcggtgaag tggtgaccac catggctatc actgttgcac gtgagttcaa aacggaaaat 721 attgtgtaca ttggtagcag cttccacaac aatgcgctgc tgcgcaaagt tgtcgaagac 781 tacaccgtgc tgcgtggctg caaaccttat tatgtagaaa acggtgcgtt ctctggcgct 841 atcggcgctc tgtatctgga aaaggggtct gactacaaag acgatgacga caagtgatac 901 tagagttgac agctagctca gtcctaggta ttgtgctagc tactagagaa agaggagaaa 961 tactagagga ttcccgcatt gacagcgcgt gcgttgcaag gcaacaatgg actcaaatgt 1021 ctcggaatcg ctgacgattc ccaggtttct ccggcaagca tagcgcatgg cgtctccatg 1081 cgagaatgtc gcgcttgccg gataaaaggg gagccgctat cggaatggac gcaagccacg 1141 gccgcagcag gtgcggtcga gggcttccag ccagttccag ggcagatgtg ccggcagacc 1201 ctcccgcttt gggggaggcg caagccgggt ccattcggat agcatctccc catgcaaagt 1261 gccggccagg gcaatgcccg gagccggttc gaatagtgac ggcagagaga caatcaaatc 1321 atggctactg ggaaaggagc cgccgccagc acccaggaag gcaaaagcca accatttaaa 1381 gtgaccccag gcccgttcga tcctgccacc tggctggaat ggtcacgtca atggcaaggt 1441 acggagggca acggccatgc ggcagcgtcc ggcatccctg ggcttgatgc actggctggt 1501 gttaaaattg cgcctgccca attgggagac atccaacagc gttatatgaa agatttttcg 1561 gcgctgtggc aggcgatggc ggaaggtaaa gcagaggcga cgggaccgct gcatgaccgt 1621 cgctttgctg gcgacgcctg gcgtaccaat ttgccctatc gttttgcggc agccttctac 1681 ctgctgaacg ctcgtgccct gactgagctg gcagatgctg tcgaagcgga tgccaaaacc 1741 cgccaacgca tccgttttgc catctcacag tgggtcgacg caatgtcacc ggccaacttc 1801 ctggccacca atccggaagc acagcgcctc ctgattgaaa gcggcgggga atcgctgcgc 1861 gccggcgttc gtaacatgat ggaagatctg acccgtggta agatttcgca gacggatgag 1921 tcggccttcg aggtgggtcg caatgttgca gtgacagaag gcgcggtggt gtttgagaac 1981 gaatactttc aacttctcca gtataaacca ctgacggata aagtgcatgc gcgcccattg 2041 ttaatggttc ccccgtgcat taataaatac tatatcctgg atcttcagcc ggaaagctcc 2101 ttagtgcgcc acgtggtgga acaggggcat actgtgtttt tagttagctg gcgcaatcca 2161 gatgcctcaa tggccggcag tacctgggat gactacattg aacacgcggc tattcgcgcg 2221 atcgaagtgg cacgcgacat ctcaggtcag gacaagatta acgtactcgg cttttgcgtc 2281 ggtggcacta tcgtgagcac cgctctggcg gtactggctg cgcgtggtga acaccccgca 2341 gcgtcggtta cgcttctgac cacactgttg gatttcgccg acactggcat cttggatgta 2401 tttgtcgatg agggccacgt tcagctgcgc gaggccactc tgggtggcgg cgccggcgca 2461 ccgtgcgctc tgctgcgcgg tctggagctc gcgaatactt tttctttctt acgcccgaac 2521 gatcttgtct ggaattacgt agttgacaac tatctcaaag gtaacacgcc cgtgccgttt 2581 gatttattat tctggaatgg ggatgcgacc aacctgccgg gtccttggta ttgttggtat 2641 ctccgtcata catacttaca gaatgaatta aaggtcccag gtaaacttac tgtgtgtggt 2701 gtcccggtag atctggcgag tatcgacgtg ccgacctaca tttatgggag tcgtgaagat 2761 catattgtgc cgtggaccgc cgcgtacgct tctaccgcgc ttctggcaaa caaattacgt 2821 ttcgttctgg gtgccagcgg ccacattgcg ggtgttatta atcctccggc caaaaataaa 2881 cgctcgcatt ggacaaacga cgccttgccg gagagccctc agcagtggtt ggcgggtgcc 2941 attgagcacc atggaagttg gtggccggac tggacagcct ggctggcggg ccaagcaggc 3001 gcaaaacgcg ctgcgccagc caactatggc aacgcgcgct accgcgcgat tgaacccgcg 3061 cctggccgct atgtaaaagc aaaagcgtga cgcttgcatg agtgccggcg tgcgtcatgc 3121 acggcgccgg caggcctgca cgttccctcc cgtttccatt gaaaggacta cacaatgacc 3181 gatgttgtta tcgtcagtgc cgcgcgcacc gccgtgggta aatttggcgg cagtctggcg 3241 aaaatcccgg cgccagaatt gggcgcggtg gtaatcaaag cggccctgga acgcgccggt 3301 gtgaaacctg agcaggtgag cgaagtaatt atgggtcagg ttctgaccgc gggcagcggg 3361 caaaatccgg cgcgtcaggc tgcgattaag gcaggccttc cggcgatggt gcccgcgatg 3421 accattaaca aagtatgcgg atcgggactg aaagcagtga tgctggccgc taacgccatt 3481 atggcaggcg acgcggaaat tgtggtcgct ggcggacagg agaatatgtc agccgcgcct 3541 catgtgctgc cgggtagccg cgacggcttt cgcatgggtg atgcaaaact ggtcgatact 3601 atgattgttg acggcttatg ggatgtgtac aaccagtacc acatgggcat caccgccgag 3661 aacgtggcca aggaatacgg catcacacgc gaggcgcagg atgagttcgc cgtcggctcg 3721 cagaacaagg ccgaagccgc gcagaaggcc ggcatgtttg acgaagagat cgtcccggtg 3781 ctgatcccgc agcgcaaggg cgacccggtg gccttcaaga ccgacgagtt cgtgcgccag 3841 ggcgccacgc tggacagcat gtccggcctc aagcccgcct tcgacaaggc cggcacggtg 3901 accgcggcca acgcctcggg cctgaacgac ggcgccgccg cggtggtggt gatgtcggcg 3961 gccaaggcca aggaactggg cctgaccccg ctggccacga tcaagagcta tgccaacgcc 4021 ggtgtcgatc ccaaggtgat gggcatgggc ccggtgccgg cctccaagcg cgccctgtcg 4081 cgcgccgagt ggaccccgca agacctggac ctgatggaga tcaacgaggc ctttgccgcg 4141 caggcgctgg cggtgcacca gcagatgggc tgggacacct ccaaggtcaa tgtgaacggc 4201 ggcgccatcg ccatcggcca cccgatcggc gcgtcgggct gccgtatcct ggtgacgctg 4261 ctgcacgaga tgaagcgccg tgacgcgaag aagggcctgg cctcgctgtg catcggcggc 4321 ggcatgggcg tggcgctggc agtcgagcgc aaataaggaa ggggttttcc ggggccgcgc 4381 gcggttggcg cggacccggc gacgataacg aagccaatca aggagtggac atgactcagc 4441 gcattgcgta tgtgaccggc ggcatgggtg gtatcggaac cgccatttgc cagcggctgg 4501 ccaaggatgg ctttcgtgtg gtggccggtt gcggccccaa ctcgccgcgc cgcgaaaagt 4561 ggctggagca gcagaaggcc ctgggcttcg atttcattgc ctcggaaggc aatgtggctg 4621 actgggactc gaccaagacc gcattcgaca aggtcaagtc cgaggtcggc gaggttgatg 4681 tgctgatcaa caacgccggt atcacccgcg acgtggtgtt ccgcaagatg acccgcgccg 4741 actgggatgc ggtgatcgac accaacctga cctcgctgtt caacgtcacc aagcaggtga 4801 tcgacggcat ggccgaccgt ggctggggcc gcatcgtcaa catctcgtcg gtgaacgggc 4861 agaagggcca gttcggccag accaactact ccaccgccaa ggccggcctg catggcttca 4921 ccatggcact ggcgcaggaa gtggcgacca agggcgtgac cgtcaacacg gtctctccgg 4981 gctatatcgc caccgacatg gtcaaggcga tccgccagga cgtgctcgac aagatcgtcg 5041 cgacgatccc ggtcaagcgc ctgggcctgc cggaagagat cgcctcgatc tgcgcctggt 5101 tgtcgtcgga ggagtccggt ttctcgaccg gcgccgactt ctcgctcaac ggcggcctgc 5161 atatgggctg acctgc //