LOCUS BBa_K1692024 3703 bp DNA linear 25-Nov-2024 DEFINITION Autolysis ACCESSION BBa_K1692024 VERSION BBa_K1692024.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 660..691 /label=LVA CDS 81..659 /label=luxI misc_feature 81..698 /label=BBa_C0061 misc_feature 699..723 /label=Help:Barcodes CDS 81..698 /label=BBa_C0061 stem_loop 743..786 /label=stem_loop misc_feature 732..811 /label=BBa_B0010 terminator 732..811 /label=BBa_B0010 stem_loop 827..846 /label=T7 TE misc_feature 853..853 /label=stop polyA_site 847..860 /label=polya misc_feature 820..860 /label=BBa_B0012 terminator 820..860 /label=BBa_B0012 terminator 732..860 /label=BBa_B0015 misc_feature 63..860 /label=BBa_K805016 promoter 869..903 /label=BBa_J23102 misc_feature 916..919 /label=conserved RBS 912..923 /label=BBa_B0034 misc_feature 930..931 /label=prefix misc_feature 1103..1103 /label=T misc_feature 1421..1421 /label=A CDS 930..1679 /label=luxR misc_feature 930..1685 /label=BBa_C0062 misc_feature 1686..1710 /label=Help:Barcodes CDS 930..1685 /label=BBa_C0062 stem_loop 1730..1773 /label=stem_loop misc_feature 1719..1798 /label=BBa_B0010 terminator 1719..1798 /label=BBa_B0010 stem_loop 1814..1833 /label=T7 TE misc_feature 1840..1840 /label=stop polyA_site 1834..1847 /label=polya misc_feature 1807..1847 /label=BBa_B0012 terminator 1807..1847 /label=BBa_B0012 terminator 1719..1847 /label=BBa_B0015 misc_feature 1856..1875 /label=LuxR/HSL promoter 1875..1880 /label=-35 promoter 1897..1902 /label=-10 misc_feature 1908..1908 /label=start misc_feature 1856..1910 /label=BBa_R0062 promoter 1856..1910 /label=BBa_R0062 CDS 1935..2591 /label=T4 holin CDS 1919..2594 /label=BBa_K112805 CDS 2619..3113 /label=T4 Endolysin CDS 2603..3116 /label=BBa_K112806 stem_loop 3136..3179 /label=stem_loop misc_feature 3125..3204 /label=BBa_B0010 terminator 3125..3204 /label=BBa_B0010 stem_loop 3220..3239 /label=T7 TE misc_feature 3246..3246 /label=stop polyA_site 3240..3253 /label=polya misc_feature 3213..3253 /label=BBa_B0012 terminator 3213..3253 /label=BBa_B0012 promoter 3262..3296 /label=BBa_J23116 CDS 3319..3612 /label=T4 antiholin CDS 3305..3615 /label=BBa_K112807 stem_loop 3635..3678 /label=stem_loop misc_feature 3624..3703 /label=BBa_B0010 terminator 3624..3703 /label=BBa_B0010 misc_feature 1919..3703 /label=BBa_K112808 misc_feature 869..3703 /label=BBa_K317039 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc 121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg 181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag 241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta 301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc 361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga 421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg 481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa 541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata 601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg 661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 721 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 781 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 841 tgggcctttc tgcgtttata tactagagtt gacagctagc tcagtcctag gtactgtgct 901 agctactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca 961 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg 1021 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta 1081 tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg caatattatg 1141 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac 1201 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta 1261 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca 1321 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat 1381 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa 1441 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt 1501 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg 1561 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct 1621 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt 1681 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa 1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 1801 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg 1861 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagct 1921 taaaaggagg gtctatggca gcacctagaa tatcattttc gccctctgat attctatttg 1981 gtgttctcga tcgcttgttc aaagataacg ctaccgggaa ggttcttgct tcccgggtag 2041 ctgtcgtaat tcttttgttt ataatggcga ttgtttggta taggggagat agtttctttg 2101 agtactataa gcaatcaaag tatgaaacat acagtgaaat tattgaaaag gaaagaactg 2161 cacgctttga atctgtcgcc ctggaacaac tccagatagt tcatatatca tctgaggcag 2221 actttagtgc ggtgtattct ttccgcccta aaaacttaaa ctattttgtt gatattatag 2281 catacgaagg aaaattacct tcaacaataa gtgaaaaatc acttggagga tatcctgttg 2341 ataaaactat ggatgaatat acagttcatt taaatggacg tcattattat tccaactcaa 2401 aatttgcttt tttaccaact aaaaagccta ctcccgaaat aaactacatg tacagttgtc 2461 catattttaa tttggataat atctatgctg gaacgataac catgtactgg tatagaaatg 2521 atcatataag taatgaccgc cttgaatcaa tatgtgctca ggcggccaga atattaggaa 2581 gggctaaata attatactag agatacttag gaggtattat gaatatattt gaaatgttac 2641 gtatagatga aggtcttaga cttaaaatct ataaagacac agaaggctat tacactattg 2701 gcatcggtca tttgcttaca aaaagtccat cacttaatgc tgctaaatct gaattagata 2761 aagctattgg gcgtaattgc aatggtgtaa ttacaaaaga tgaggctgaa aaactcttta 2821 atcaggatgt tgatgctgct gttcgcggaa tcctgagaaa tgctaaatta aaaccggttt 2881 atgattctct tgatgcggtt cgtcgctgtg cattgattaa tatggttttc caaatgggag 2941 aaaccggtgt ggcaggattt actaactctt tacgtatgct tcaacaaaaa cgctgggatg 3001 aagcagcagt taacttagct aaaagtagat ggtataatca aacacctaat cgcgcaaaac 3061 gagtcattac aacgtttaga actggcactt gggacgcgta taaaaatcta taaagctact 3121 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 3181 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 3241 ttctgcgttt atatactaga gttgacagct agctcagtcc tagggactat gctagctact 3301 agaggatagg aggcctttat ggccttaaaa gcaacagcac tttttgccat gctaggattg 3361 tcatttgttt tatctccatc gattgaagcg aatgtcgatc ctcattttga taaatttatg 3421 gaatctggta ttaggcacgt ttatatgctt tttgaaaata aaagcgtaga atcgtctgaa 3481 caattctata gttttatgag aacgacctat aaaaatgacc cgtgctcttc tgattttgaa 3541 tgtatagagc gaggcgcgga gatggcacaa tcatacgcta gaattatgaa cattaaattg 3601 gagactgaat gaaattacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3661 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctc //