##gff-version 3
##sequence-region BBa_K1692024 1 3703
BBa_K1692024	.	engineered_region	869	3703	.	+	0	ID=BBa_K317039;Name=BBa_K317039
BBa_K1692024	.	promoter	869	903	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K317039
BBa_K1692024	.	engineered_region	1919	3703	.	+	0	ID=BBa_K112808;Name=BBa_K112808;Parent=BBa_K317039
BBa_K1692024	.	CDS	3305	3615	.	+	0	ID=BBa_K112807;Name=BBa_K112807;Parent=BBa_K112808
BBa_K1692024	.	CDS	3319	3612	.	+	0	ID=annotation1999019;Name=T4 antiholin;Parent=BBa_K112807
BBa_K1692024	.	terminator	3213	3253	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K112808
BBa_K1692024	.	polyA_site	3240	3253	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1692024	.	stem_loop	3220	3239	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1692024	.	engineered_region	3213	3253	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1692024	.	stop_codon	3246	3246	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1692024	.	terminator	3624	3703	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K112808
BBa_K1692024	.	stem_loop	3635	3678	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1692024	.	engineered_region	3624	3703	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1692024	.	promoter	3262	3296	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K112808
BBa_K1692024	.	terminator	3125	3204	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K112808
BBa_K1692024	.	stem_loop	3136	3179	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1692024	.	engineered_region	3125	3204	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1692024	.	CDS	2603	3116	.	+	0	ID=BBa_K112806;Name=BBa_K112806;Parent=BBa_K112808
BBa_K1692024	.	CDS	2619	3113	.	+	0	ID=annotation1999018;Name=T4 Endolysin;Parent=BBa_K112806
BBa_K1692024	.	CDS	1919	2594	.	+	0	ID=BBa_K112805;Name=BBa_K112805;Parent=BBa_K112808
BBa_K1692024	.	CDS	1935	2591	.	+	0	ID=annotation1999005;Name=T4 holin;Parent=BBa_K112805
BBa_K1692024	.	CDS	930	1685	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317039
BBa_K1692024	.	CDS	930	1679	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1692024	.	point_mutation	1421	1421	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1692024	.	point_mutation	1103	1103	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1692024	.	engineered_tag	1686	1710	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1692024	.	engineered_region	930	1685	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1692024	.	engineered_region	930	931	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1692024	.	ribosome_entry_site	912	923	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317039
BBa_K1692024	.	conserved_region	916	919	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1692024	.	terminator	1719	1847	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317039
BBa_K1692024	.	terminator	1807	1847	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1692024	.	polyA_site	1834	1847	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1692024	.	stem_loop	1814	1833	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1692024	.	engineered_region	1807	1847	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1692024	.	stop_codon	1840	1840	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1692024	.	terminator	1719	1798	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1692024	.	stem_loop	1730	1773	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1692024	.	engineered_region	1719	1798	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1692024	.	promoter	1856	1910	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317039
BBa_K1692024	.	start_codon	1908	1908	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1692024	.	promoter	1875	1880	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1692024	.	promoter	1897	1902	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1692024	.	engineered_region	1856	1910	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1692024	.	non_covalent_binding_site	1856	1875	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1692024	.	engineered_region	63	860	.	+	0	ID=BBa_K805016;Name=BBa_K805016
BBa_K1692024	.	CDS	81	698	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1692024	.	tag	660	691	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1692024	.	engineered_region	81	698	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1692024	.	engineered_tag	699	723	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1692024	.	CDS	81	659	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1692024	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1692024	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1692024	.	terminator	732	860	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1692024	.	terminator	820	860	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1692024	.	polyA_site	847	860	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1692024	.	stem_loop	827	846	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1692024	.	engineered_region	820	860	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1692024	.	stop_codon	853	853	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1692024	.	terminator	732	811	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1692024	.	stem_loop	743	786	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1692024	.	engineered_region	732	811	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1692024	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1692024	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1692024	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1692024	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1692024	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1692024	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K1692024
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagt
tgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccg
acgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgac
taaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatccta
gataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattcta
actccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaaga
agcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtttt
gcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctc
tagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttt
agcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccat
ttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattg
attgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgt
tatagtcgaataaatactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattct
atttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattctt
ttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacataca
gtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatc
tgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaagga
aaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatt
taaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacat
gtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatata
agtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatact
taggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaagg
ctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagct
attgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttc
gcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatat
ggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaa
gcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaa
ctggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggccttt
atggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcg
atcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtc
tgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggc
gcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc