BBa_K1692025 1 BBa_K1692025 pcotZ-cotZ 2015-09-13T11:00:00Z 2015-09-14T12:05:43Z Bacillus Subtilis cotZ spore coat protein Bacillus Subtilis spore coat protein form the LMU Munich 2012 iGEM team's project sporobeads. This is the basis for a composite part. false false _2110_ 26512 26512 9 false n/a false Charles Calvet annotation2454767 1 cotZ range2454767 1 1 450 annotation2454768 1 GS linker range2454768 1 451 486 BBa_K1692027 1 BBa_K1692027 CIPA-MSA 2015-09-13T11:00:00Z 2015-09-24T12:31:15Z iGEM team 2014 Cellulose binding domain adapted from imperial 2014 iGEM team. Last part of our composite part BBa_BBa_K1692028 false false _2110_ 11076 26512 9 false deleted ecoRI site at the end of the sequence false Charles Calvet, Thai Nguyen annotation2454773 1 CIPA (-ecoR1) range2454773 1 1 519 BBa_K1692026 1 BBa_K1692026 aeBLUE - GS linker 2015-09-13T11:00:00Z 2015-09-14T12:15:45Z aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. aeBlue - GS linker to be part of the BBa_K1692028 composite part false false _2110_ 26512 26512 9 false n/a false Charles Calvet annotation2454770 1 misc range2454770 1 694 714 annotation2454769 1 aeBlue range2454769 1 1 693 BBa_K1692028 1 BBa_K1692028 cotZ-aeBlue-CIPA 2015-09-13T11:00:00Z 2015-09-14T12:29:47Z cotZ from the LMU munich 2012 iGEM team (http://parts.igem.org/Part:BBa_K823032). aeBlue from iGEM12 Uppsala (http://parts.igem.org/Part:BBa_K864401), CIPA from our lab The goal of our project BioHYDRA was to replace all the parts of HYDRAs by biologically produced substances. We sought out to replace polyamide tape by bacterially cellulose, and the glue by cellulose binding domains on the surface of the spore coat. Thus, the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. false false _2110_ 26512 26512 9 false This part was ligated into the pSBbs1c bacillus backbone from the LMU Munich 2012 iGEM team's Bacillus Bio Brick Box (http://parts.igem.org/wiki/index.php?title=Part:BBa_K823023) false Charles Calvet component2454806 1 BBa_K1692026 component2454803 1 BBa_K1692025 component2454808 1 BBa_K1692027 annotation2454803 1 BBa_K1692025 range2454803 1 1 486 annotation2454808 1 BBa_K1692027 range2454808 1 1215 1733 annotation2454806 1 BBa_K1692026 range2454806 1 493 1206 BBa_K1692025_sequence 1 atgttactagagaaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcattactccggcggtggcagcggtggtggcagctccagcggc BBa_K1692026_sequence 1 atggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaacaatttcatcttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcacggcagcggtgcgggcagcggc BBa_K1692027_sequence 1 acctgtttcaggcaacttgaaggtcgagttctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttcctagagtcacactggctcacctact BBa_K1692028_sequence 1 atgttactagagaaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcattactccggcggtggcagcggtggtggcagctccagcggctactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaacaatttcatcttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcacggcagcggtgcgggcagcggctactagagacctgtttcaggcaacttgaaggtcgagttctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttcctagagtcacactggctcacctact igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z