BBa_K1694013 1 BBa_K1694013 OmpA-scFv(Anti-VEGF) 2015-09-14T11:00:00Z 2015-09-18T08:52:26Z Assemble We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU component2457342 1 BBa_K1694003 component2457341 1 BBa_K1694002 annotation2457342 1 BBa_K1694003 range2457342 1 447 1193 annotation2457341 1 BBa_K1694002 range2457341 1 1 438 BBa_K1694003 1 BBa_K1694003 Single-chain variable fragment (Anti-VEGF) 2015-09-14T11:00:00Z 2015-09-18T07:47:19Z Artificial synthesis We selected the single chain variable fragments (scFv) of monoclonal antibodies Bevacizumab false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1694002 1 BBa_K1694002 Lpp-OmpA-N 2015-09-14T11:00:00Z 2015-09-18T09:58:58Z Artificial synthesis To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU BBa_K1694023 1 BBa_K1694023 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-VEGF) 2015-09-14T11:00:00Z 2015-09-18T10:05:54Z Assemble By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Bevacizumab) outside the E.coli cell membrane. false false _2112_ 27160 28523 9 false No false CHIH-HSUAN HSU component2457369 1 BBa_K1694013 component2457364 1 BBa_J23101 component2457366 1 BBa_B0034 annotation2457366 1 BBa_B0034 range2457366 1 44 55 annotation2457364 1 BBa_J23101 range2457364 1 1 35 annotation2457369 1 BBa_K1694013 range2457369 1 64 1256 BBa_K1694023_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattcagatgacacagagcccgagcagcctgagcgcaagcgttggtgatcgcgttaccattacttgttctgccagccaggatatcagcaattatctgaactggtatcagcagaagccgggtaaagccccgaaagtgctgatctacttcaccagcagcctgcatagcggcgttccgagtcgctttagcggtagcggcagcggcaccgatttcaccctgaccattagcagcttacaaccggaagattttgccacctactactgccagcagtatagcaccgtgccgtggacctttggccagggtaccaaagtggagatcaaaggcggtagcagtcgtagcagtagtagtggtggtggtggcagcggtggtggtggtgaagtgcagctggttgaaagcggtggcggtctggttcagccgggtggtagtctgcgcctgagctgtgcagccagcggctatacctttaccaactacggcatgaattgggtgcgtcaggccccgggtaaaggtctggaatgggtgggctggatcaatacctataccggcgaaccgacctacgccgccgactttaaacgccgcttcacctttagcctggacaccagcaaaagcaccgcctatttacaaatgaatagcctgcgcgccgaagataccgcagtgtactactgcgccaagtatccgcactactacggcagcagccattggtactttgacgtttggggccagggcaccctggttaccgtgagcagttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1694013_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattcagatgacacagagcccgagcagcctgagcgcaagcgttggtgatcgcgttaccattacttgttctgccagccaggatatcagcaattatctgaactggtatcagcagaagccgggtaaagccccgaaagtgctgatctacttcaccagcagcctgcatagcggcgttccgagtcgctttagcggtagcggcagcggcaccgatttcaccctgaccattagcagcttacaaccggaagattttgccacctactactgccagcagtatagcaccgtgccgtggacctttggccagggtaccaaagtggagatcaaaggcggtagcagtcgtagcagtagtagtggtggtggtggcagcggtggtggtggtgaagtgcagctggttgaaagcggtggcggtctggttcagccgggtggtagtctgcgcctgagctgtgcagccagcggctatacctttaccaactacggcatgaattgggtgcgtcaggccccgggtaaaggtctggaatgggtgggctggatcaatacctataccggcgaaccgacctacgccgccgactttaaacgccgcttcacctttagcctggacaccagcaaaagcaccgcctatttacaaatgaatagcctgcgcgccgaagataccgcagtgtactactgcgccaagtatccgcactactacggcagcagccattggtactttgacgtttggggccagggcaccctggttaccgtgagcagttaa BBa_K1694002_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1694003_sequence 1 gatattcagatgacacagagcccgagcagcctgagcgcaagcgttggtgatcgcgttaccattacttgttctgccagccaggatatcagcaattatctgaactggtatcagcagaagccgggtaaagccccgaaagtgctgatctacttcaccagcagcctgcatagcggcgttccgagtcgctttagcggtagcggcagcggcaccgatttcaccctgaccattagcagcttacaaccggaagattttgccacctactactgccagcagtatagcaccgtgccgtggacctttggccagggtaccaaagtggagatcaaaggcggtagcagtcgtagcagtagtagtggtggtggtggcagcggtggtggtggtgaagtgcagctggttgaaagcggtggcggtctggttcagccgggtggtagtctgcgcctgagctgtgcagccagcggctatacctttaccaactacggcatgaattgggtgcgtcaggccccgggtaaaggtctggaatgggtgggctggatcaatacctataccggcgaaccgacctacgccgccgactttaaacgccgcttcacctttagcctggacaccagcaaaagcaccgcctatttacaaatgaatagcctgcgcgccgaagataccgcagtgtactactgcgccaagtatccgcactactacggcagcagccattggtactttgacgtttggggccagggcaccctggttaccgtgagcagttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z