BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1694024 1 BBa_K1694024 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T08:07:32Z Assembly By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Cetuximab) outside the E.coli cell membrane. false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU component2457382 1 BBa_J23101 component2457384 1 BBa_B0034 component2457387 1 BBa_K1694014 annotation2457382 1 BBa_J23101 range2457382 1 1 35 annotation2457384 1 BBa_B0034 range2457384 1 44 55 annotation2457387 1 BBa_K1694014 range2457387 1 64 1244 BBa_K1694004 1 BBa_K1694004 Single-chain variable fragment (Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T07:29:10Z Artificial synthesis We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab false false _2112_ 28523 28523 9 true No false CHIH-HSUAN HSU BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1694014 1 BBa_K1694014 OmpA-scFv(Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T09:37:51Z Assemble We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab false false _2112_ 27153 28523 9 false No false CHIH-HSUAN HSU component2457313 1 BBa_K1694002 component2457314 1 BBa_K1694004 annotation2457314 1 BBa_K1694004 range2457314 1 447 1181 annotation2457313 1 BBa_K1694002 range2457313 1 1 438 BBa_K1694002 1 BBa_K1694002 Lpp-OmpA-N 2015-09-14T11:00:00Z 2015-09-18T09:58:58Z Artificial synthesis To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU BBa_K1694024_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_K1694014_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1694002_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg BBa_K1694004_sequence 1 gatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z