LOCUS       BBa_K1694034            2114 bp    DNA     linear       22-Dec-2024
DEFINITION  Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+GFP+J61048
ACCESSION   BBa_K1694034
VERSION     BBa_K1694034.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..501
                     /label=BBa_K1694002
     CDS             510..1244
                     /label=BBa_K1694004
     misc_feature    64..1244
                     /label=BBa_K1694014
     misc_feature    1..1244
                     /label=BBa_K1694024
     RBS             1253..1267
                     /label=RBS-1\Strong
     RBS             1260..1264
                     /label=RBS
     misc_feature    1253..1267
                     /label=BBa_B0030
     RBS             1253..1267
                     /label=BBa_B0030
     CDS             1274..1993
                     /label=GFP protein
     CDS             1274..1993
                     /label=BBa_E0040
     terminator      2002..2114
                     /label=BBa_J61048
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagaaagcaa ccaagctggt tctgggtgcc gtgattctgg gcagtaccct gttagcaggt
      121 tgttctagca atgccaaaat cgaccaaggc atcaacaaca atggcccgac ccacgaaaac
      181 cagctgggtg ccggtgcctt tggtggttat caggtgaacc cgtacgtggg ctttgaaatg
      241 ggctatgatt ggctgggccg catgccgtac aaaggcagtg tggagaacgg cgcctataaa
      301 gcacagggcg tgcagctgac agcaaaactg ggctacccta ttaccgacga cctggacatc
      361 tacacacgct taggcggcat ggtgtggcgc gccgatacca agagcaacgt gtacggcaag
      421 aaccacgata ccggcgtgag tccggtgttt gccggcggtg tggagtatgc aatcaccccg
      481 gaaattgcca cacgtccatg gtactagagg atattctgct gacccagagc ccggtgatcc
      541 tgagcgtgag tccgggtgaa cgtgtgagct tcagttgccg tgccagccag agcatcggta
      601 ccaacatcca ctggtatcag cagcgcacca acggtagtcc gcgcctgctg atcaaatatg
      661 ccagcgagag catcagcggc atcccgagcc gctttagtgg tagcggcagc ggcaccgatt
      721 ttaccctgag catcaacagc gtggaaagcg aagacatcgc agattattat tgccagcaga
      781 acaataattg gccgaccacc ttcggcgccg gtaccaaact ggaactgaaa ggcggtagca
      841 gccgtagtag cagcagtggt ggtggtggta gtggtggcgg tggccaggtt cagctgaaac
      901 agagcggtcc gggtctggtg cagcctagcc agagcctgag cattacctgc accgtgagcg
      961 gctttagcct gaccaattac ggcgtgcatt gggtgcgtca gagcccgggc aagggtctgg
     1021 aatggctggg tgttatctgg agcggcggca ataccgatta caacaccccg ttcaccagcc
     1081 gcctgagtat taacaaagat aatagcaaaa gccaggtctt ttttaaaatg aatagcttac
     1141 agagcaatga caccgcaatt tattactgcg cccgcgccct gacctactat gattatgagt
     1201 tcgcatactg gggtcagggc accctggtga cagtgagtgc ataatactag agattaaaga
     1261 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1321 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1381 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1441 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1501 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1561 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1621 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1681 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1741 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1801 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1861 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1921 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1981 atacaaataa taatactaga gccggcttat cggtcagttt cacctgattt acgtaaaaac
     2041 ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct
     2101 atacccaaaa gaaa
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