BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1694004
1
BBa_K1694004
Single-chain variable fragment (Anti-EGFR)
2015-09-14T11:00:00Z
2015-09-18T07:29:10Z
Artificial synthesis
We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
false
false
_2112_
28523
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_K1694024
1
BBa_K1694024
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)
2015-09-14T11:00:00Z
2015-09-18T08:07:32Z
Assembly
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Cetuximab) outside the E.coli cell membrane.
false
false
_2112_
28523
28523
9
false
No
false
CHIH-HSUAN HSU
component2457387
1
BBa_K1694014
component2457382
1
BBa_J23101
component2457384
1
BBa_B0034
annotation2457387
1
BBa_K1694014
range2457387
1
64
1244
annotation2457382
1
BBa_J23101
range2457382
1
1
35
annotation2457384
1
BBa_B0034
range2457384
1
44
55
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1694002
1
BBa_K1694002
Lpp-OmpA-N
2015-09-14T11:00:00Z
2015-09-18T09:58:58Z
Artificial synthesis
To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_K1694034
1
BBa_K1694034
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+GFP+J61048
2015-09-14T11:00:00Z
2015-09-20T05:20:04Z
Assembly
O
false
false
_2112_
22485
28523
9
true
No
false
CHIH-HSUAN HSU
component2457448
1
BBa_K1694024
component2457454
1
BBa_J61048
component2457450
1
BBa_B0030
component2457453
1
BBa_E0040
annotation2457453
1
BBa_E0040
range2457453
1
1274
1993
annotation2457448
1
BBa_K1694024
range2457448
1
1
1244
annotation2457454
1
BBa_J61048
range2457454
1
2002
2114
annotation2457450
1
BBa_B0030
range2457450
1
1253
1267
BBa_K1694014
1
BBa_K1694014
OmpA-scFv(Anti-EGFR)
2015-09-14T11:00:00Z
2015-09-18T09:37:51Z
Assemble
We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
false
false
_2112_
27153
28523
9
false
No
false
CHIH-HSUAN HSU
component2457314
1
BBa_K1694004
component2457313
1
BBa_K1694002
annotation2457314
1
BBa_K1694004
range2457314
1
447
1181
annotation2457313
1
BBa_K1694002
range2457313
1
1
438
BBa_K1694024_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
BBa_K1694014_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
BBa_K1694034_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1694002_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
BBa_K1694004_sequence
1
gatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z