BBa_K1694005
1
BBa_K1694005
Single-chain variable fragment (Anti-HER2)
2015-09-14T11:00:00Z
2015-09-18T07:36:18Z
Artificial synthesis
We selected the single chain variable fragments (scFv) of monoclonal antibodies Trastuzumab
false
false
_2112_
28523
28523
9
false
No
false
CHIH-HSUAN HSU
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1694035
1
BBa_K1694035
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)+B0030+GFP+J61048
2015-09-14T11:00:00Z
2015-09-18T10:05:39Z
assemble
The engineered E.coli could express both scFv of anti-Her2 and blue flourescent.
false
false
_2112_
27160
27160
9
false
No
false
Chen Yu, Chang
component2457497
1
BBa_K1694005
component2457503
1
BBa_J61048
component2457496
1
BBa_K1694002
component2457495
1
BBa_B0034
component2457493
1
BBa_J23101
component2457502
1
BBa_E0040
component2457499
1
BBa_B0030
annotation2457497
1
BBa_K1694005
range2457497
1
508
1089
annotation2457493
1
BBa_J23101
range2457493
1
1
35
annotation2457495
1
BBa_B0034
range2457495
1
44
55
annotation2457499
1
BBa_B0030
range2457499
1
1098
1112
annotation2457496
1
BBa_K1694002
range2457496
1
64
501
annotation2457503
1
BBa_J61048
range2457503
1
1847
1959
annotation2457502
1
BBa_E0040
range2457502
1
1119
1838
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1694002
1
BBa_K1694002
Lpp-OmpA-N
2015-09-14T11:00:00Z
2015-09-18T09:58:58Z
Artificial synthesis
To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1694035_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1694005_sequence
1
atgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1694002_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z