BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J61048 1 BBa_J61048 [rnpB-T1] Terminator 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1694007 1 BBa_K1694007 FadL-GBP 2015-09-14T11:00:00Z 2015-09-18T10:01:10Z Artificial synthesis GBP(Gold-Binding polypeptide) FadL as an anchoring motif (to display the GBP on the surface of E.coli, and consequently to immobilize the cells on the gold surface. false false _2112_ 27160 27160 9 true No false Chen Yu, Chang BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_K1694037 1 BBa_K1694037 Pcons+B0034+FadL-GBP+B0030+GFP+J61048 2015-09-14T11:00:00Z 2015-09-20T06:30:17Z assemble The engineered E.coli would display FadL-GBP and emit blue light. false false _2112_ 27160 27160 9 true No false Chen Yu, Chang component2457545 1 BBa_B0034 component2457548 1 BBa_B0030 component2457552 1 BBa_J61048 component2457551 1 BBa_E0040 component2457546 1 BBa_K1694007 component2457543 1 BBa_J23101 annotation2457545 1 BBa_B0034 range2457545 1 44 55 annotation2457548 1 BBa_B0030 range2457548 1 1354 1368 annotation2457543 1 BBa_J23101 range2457543 1 1 35 annotation2457552 1 BBa_J61048 range2457552 1 2103 2215 annotation2457546 1 BBa_K1694007 range2457546 1 62 1345 annotation2457551 1 BBa_E0040 range2457551 1 1375 2094 BBa_K1694007_sequence 1 atgagccagaaaaccctgtttaccaaaagcgcactggcagtggcagtggcactgattagcacccaggcctggagcgccggtttccaactgaacgagtttagcagcagcggtctgggtcgtgcatatagcggtgagggtgccattgccgatgacgccggtaatgtgagccgcaatcctgccctgatcaccatgttcgatcgcccgacatttagcgccggcgccgtttatattgatccggacgtgaatatcagcggcaccagtccgagtggccgtagcctgaaggcagacaacattgcaccgaccgcctgggttccgaacatgcacttcgttgcaccgatcaatgaccagttcggttggggcgccagcattaccagcaactatggtctggccaccgagttcaatgatacctacgcaggtggtagcgtgggtggtaccacagatctggagacaatgaacctgaatctgagcggtgcctatcgtctgaataacgcatggagcttcggcctgggcttcaatgccgtttacgcccgtgccaagatcgaacgttttgcaggcgatctgggtcagctggttgcaggtcagattatgcagagcccggccggtcagacacagcaaggtcaggccctggcagcaaccgccaacggcatcgatagcaacaccaaaatcgcccacctgaacggcaatcagtggggctttggttggaacgccggcattctgtacgaactggacaaaaacaaccgctacgccctgacctatcgcagcgaagtgaaaatcgattttaaaggcaactattctagcgacctgaatcgcgcatttaataactatggcctgcctatccctaccgcaaccggtggtgccacccagagcggttacctgacactgaacctgccggagatgtgggaggtgagcggttacaaccgcgtggatccgcagtgggccattcactacagcctggcatataccagctggagccagttccagcagctgaaagccaccagtacaagtggtgacaccctgtttcaaaaacacgagggcttcaaagacgcatatcgtatcgcactgggcacaacctactactacgacgacaattggacatttcgcaccggcattgccttcgatgatagtccggtgccggcccagaaccgcagcattatgcacggtaagacccaagccaccagtggcaccatccagagcatgcatggtaaaacccaggcaaccagcggcaccatccaaagcatgcatggcaaaacccaggccaccagtggtaccattcagagctaataa BBa_K1694037_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgagccagaaaaccctgtttaccaaaagcgcactggcagtggcagtggcactgattagcacccaggcctggagcgccggtttccaactgaacgagtttagcagcagcggtctgggtcgtgcatatagcggtgagggtgccattgccgatgacgccggtaatgtgagccgcaatcctgccctgatcaccatgttcgatcgcccgacatttagcgccggcgccgtttatattgatccggacgtgaatatcagcggcaccagtccgagtggccgtagcctgaaggcagacaacattgcaccgaccgcctgggttccgaacatgcacttcgttgcaccgatcaatgaccagttcggttggggcgccagcattaccagcaactatggtctggccaccgagttcaatgatacctacgcaggtggtagcgtgggtggtaccacagatctggagacaatgaacctgaatctgagcggtgcctatcgtctgaataacgcatggagcttcggcctgggcttcaatgccgtttacgcccgtgccaagatcgaacgttttgcaggcgatctgggtcagctggttgcaggtcagattatgcagagcccggccggtcagacacagcaaggtcaggccctggcagcaaccgccaacggcatcgatagcaacaccaaaatcgcccacctgaacggcaatcagtggggctttggttggaacgccggcattctgtacgaactggacaaaaacaaccgctacgccctgacctatcgcagcgaagtgaaaatcgattttaaaggcaactattctagcgacctgaatcgcgcatttaataactatggcctgcctatccctaccgcaaccggtggtgccacccagagcggttacctgacactgaacctgccggagatgtgggaggtgagcggttacaaccgcgtggatccgcagtgggccattcactacagcctggcatataccagctggagccagttccagcagctgaaagccaccagtacaagtggtgacaccctgtttcaaaaacacgagggcttcaaagacgcatatcgtatcgcactgggcacaacctactactacgacgacaattggacatttcgcaccggcattgccttcgatgatagtccggtgccggcccagaaccgcagcattatgcacggtaagacccaagccaccagtggcaccatccagagcatgcatggtaaaacccaggcaaccagcggcaccatccaaagcatgcatggcaaaacccaggccaccagtggtaccattcagagctaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_B0034_sequence 1 aaagaggagaaa BBa_J61048_sequence 1 ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_B0030_sequence 1 attaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z