BBa_K1694044
type: ComponentDefinition
persistent identity: BBa K1694044
display id: BBa_K1694044
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+RFP+J61048
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T10:05:30Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: J
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27160
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_B0030
component: BBa_K1694024
component: BBa_J61048
sequence annotation: BBa_K1694024
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K1694044_sequence/1

BBa_E1010
type: Component
persistent identity: component2457516
display id: component2457516
version: 1
top level: BBa_K1694044
definition: mRFP1
access: public

BBa_B0030
type: Component
persistent identity: component2457512
display id: component2457512
version: 1
top level: BBa_K1694044
definition: BBa_B0030
access: public

BBa_K1694024
type: Component
persistent identity: component2457510
display id: component2457510
version: 1
top level: BBa_K1694044
definition: BBa_K1694024
access: public

BBa_J61048
type: Component
persistent identity: component2457517
display id: component2457517
version: 1
top level: BBa_K1694044
definition: BBa_J61048
access: public

BBa_K1694024
type: SequenceAnnotation
persistent identity: annotation2457510
display id: annotation2457510
version: 1
top level: BBa_K1694044
location: https://synbiohub.org/public/igem/BBa_K1694044/annotation2457510/range2457510/1
component: BBa_K1694024

https://synbiohub.org/public/igem/BBa_K1694044/annotation2457510/range2457510/1
type: Range
persistent identity: range2457510
display id: range2457510
version: 1
top level: BBa_K1694044
direction: direction/reverse
start: 1
end: 1244
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2457512
display id: annotation2457512
version: 1
top level: BBa_K1694044
location: https://synbiohub.org/public/igem/BBa_K1694044/annotation2457512/range2457512/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1694044/annotation2457512/range2457512/1
type: Range
persistent identity: range2457512
display id: range2457512
version: 1
top level: BBa_K1694044
direction: direction/forward
start: 1253
end: 1267
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2457516
display id: annotation2457516
version: 1
top level: BBa_K1694044
location: https://synbiohub.org/public/igem/BBa_K1694044/annotation2457516/range2457516/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1694044/annotation2457516/range2457516/1
type: Range
persistent identity: range2457516
display id: range2457516
version: 1
top level: BBa_K1694044
direction: direction/reverse
start: 1274
end: 1979
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2457517
display id: annotation2457517
version: 1
top level: BBa_K1694044
location: https://synbiohub.org/public/igem/BBa_K1694044/annotation2457517/range2457517/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K1694044/annotation2457517/range2457517/1
type: Range
persistent identity: range2457517
display id: range2457517
version: 1
top level: BBa_K1694044
direction: direction/reverse
start: 1988
end: 2100
orientation: inline

BBa_K1694024
type: ComponentDefinition
persistent identity: BBa K1694024
display id: BBa_K1694024
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T08:07:32Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Cetuximab) outside the E.coli cell membrane.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 28523
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23101
component: BBa_K1694014
component: BBa_B0034
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_K1694014
sequence: https://synbiohub.org/public/igem/BBa_K1694024_sequence/1

BBa_J23101
type: Component
persistent identity: component2457382
display id: component2457382
version: 1
top level: BBa_K1694024
definition: BBa_J23101
access: public

BBa_K1694014
type: Component
persistent identity: component2457387
display id: component2457387
version: 1
top level: BBa_K1694024
definition: BBa_K1694014
access: public

BBa_B0034
type: Component
persistent identity: component2457384
display id: component2457384
version: 1
top level: BBa_K1694024
definition: BBa_B0034
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2457382
display id: annotation2457382
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457382/range2457382/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457382/range2457382/1
type: Range
persistent identity: range2457382
display id: range2457382
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2457384
display id: annotation2457384
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457384/range2457384/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457384/range2457384/1
type: Range
persistent identity: range2457384
display id: range2457384
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1694014
type: SequenceAnnotation
persistent identity: annotation2457387
display id: annotation2457387
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457387/range2457387/1
component: BBa_K1694014

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457387/range2457387/1
type: Range
persistent identity: range2457387
display id: range2457387
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 64
end: 1244
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

BBa_K1694014
type: ComponentDefinition
persistent identity: BBa K1694014
display id: BBa_K1694014
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA-scFv(Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T09:37:51Z
mutable provenance: Assemble
owned by: james
owned by: myers
mutable description: We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27153
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1694004
component: BBa_K1694002
sequence annotation: BBa_K1694002
sequence annotation: BBa_K1694004
sequence: https://synbiohub.org/public/igem/BBa_K1694014_sequence/1

BBa_K1694004
type: Component
persistent identity: component2457314
display id: component2457314
version: 1
top level: BBa_K1694014
definition: BBa_K1694004
access: public

BBa_K1694002
type: Component
persistent identity: component2457313
display id: component2457313
version: 1
top level: BBa_K1694014
definition: BBa_K1694002
access: public

BBa_K1694002
type: SequenceAnnotation
persistent identity: annotation2457313
display id: annotation2457313
version: 1
top level: BBa_K1694014
location: https://synbiohub.org/public/igem/BBa_K1694014/annotation2457313/range2457313/1
component: BBa_K1694002

https://synbiohub.org/public/igem/BBa_K1694014/annotation2457313/range2457313/1
type: Range
persistent identity: range2457313
display id: range2457313
version: 1
top level: BBa_K1694014
direction: direction/reverse
start: 1
end: 438
orientation: inline

BBa_K1694004
type: SequenceAnnotation
persistent identity: annotation2457314
display id: annotation2457314
version: 1
top level: BBa_K1694014
location: https://synbiohub.org/public/igem/BBa_K1694014/annotation2457314/range2457314/1
component: BBa_K1694004

https://synbiohub.org/public/igem/BBa_K1694014/annotation2457314/range2457314/1
type: Range
persistent identity: range2457314
display id: range2457314
version: 1
top level: BBa_K1694014
direction: direction/reverse
start: 447
end: 1181
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1694002
type: ComponentDefinition
persistent identity: BBa K1694002
display id: BBa_K1694002
version: 1
was generated by: iGEM to SBOL conversion
description: Lpp-OmpA-N
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T09:58:58Z
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
mutable description: To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27160
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1694002_sequence/1

BBa_K1694004
type: ComponentDefinition
persistent identity: BBa K1694004
display id: BBa_K1694004
version: 1
was generated by: iGEM to SBOL conversion
description: Single-chain variable fragment (Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T07:29:10Z
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
mutable description: We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 28523
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1694004_sequence/1

https://synbiohub.org/public/igem/BBa_K1694044_sequence/1
type: Sequence
persistent identity: BBa K1694044 sequence
display id: BBa_K1694044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccggcttatcggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694024_sequence/1
type: Sequence
persistent identity: BBa K1694024 sequence
display id: BBa_K1694024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694014_sequence/1
type: Sequence
persistent identity: BBa K1694014 sequence
display id: BBa_K1694014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694002_sequence/1
type: Sequence
persistent identity: BBa K1694002 sequence
display id: BBa_K1694002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694004_sequence/1
type: Sequence
persistent identity: BBa K1694004 sequence
display id: BBa_K1694004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z