BBa_K592009
1
amilCP
amilCP, blue chromoprotein
2011-09-17T11:00:00Z
2015-05-08T01:12:48Z
Acropora millepora
Released HQ 2013
This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
false
false
_763_
0
7929
9
In stock
true
Illegal internal restriction site had to be removed (EcoRI).
false
Lei Sun
annotation2131628
1
amilCP
range2131628
1
1
666
BBa_K1694003
1
BBa_K1694003
Single-chain variable fragment (Anti-VEGF)
2015-09-14T11:00:00Z
2015-09-18T07:47:19Z
Artificial synthesis
We selected the single chain variable fragments (scFv) of monoclonal antibodies Bevacizumab
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1694002
1
BBa_K1694002
Lpp-OmpA-N
2015-09-14T11:00:00Z
2015-09-18T09:58:58Z
Artificial synthesis
To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_K1694053
1
BBa_K1694053
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-VEGF)+B0034+amilCP+B0015
2015-09-14T11:00:00Z
2015-09-18T10:06:51Z
assemble
amilCP
false
false
_2112_
27160
27160
9
true
No
false
Chen Yu, Chang
component2457599
1
BBa_K592009
component2457600
1
BBa_J61048
component2457594
1
BBa_K1694003
component2457590
1
BBa_J23101
component2457592
1
BBa_B0034
component2457593
1
BBa_K1694002
component2457596
1
BBa_B0030
annotation2457592
1
BBa_B0034
range2457592
1
44
55
annotation2457600
1
BBa_J61048
range2457600
1
1963
2075
annotation2457590
1
BBa_J23101
range2457590
1
1
35
annotation2457599
1
BBa_K592009
range2457599
1
1286
1954
annotation2457594
1
BBa_K1694003
range2457594
1
510
1256
annotation2457593
1
BBa_K1694002
range2457593
1
64
501
annotation2457596
1
BBa_B0030
range2457596
1
1265
1279
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K592009_sequence
1
atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
BBa_K1694053_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattcagatgacacagagcccgagcagcctgagcgcaagcgttggtgatcgcgttaccattacttgttctgccagccaggatatcagcaattatctgaactggtatcagcagaagccgggtaaagccccgaaagtgctgatctacttcaccagcagcctgcatagcggcgttccgagtcgctttagcggtagcggcagcggcaccgatttcaccctgaccattagcagcttacaaccggaagattttgccacctactactgccagcagtatagcaccgtgccgtggacctttggccagggtaccaaagtggagatcaaaggcggtagcagtcgtagcagtagtagtggtggtggtggcagcggtggtggtggtgaagtgcagctggttgaaagcggtggcggtctggttcagccgggtggtagtctgcgcctgagctgtgcagccagcggctatacctttaccaactacggcatgaattgggtgcgtcaggccccgggtaaaggtctggaatgggtgggctggatcaatacctataccggcgaaccgacctacgccgccgactttaaacgccgcttcacctttagcctggacaccagcaaaagcaccgcctatttacaaatgaatagcctgcgcgccgaagataccgcagtgtactactgcgccaagtatccgcactactacggcagcagccattggtactttgacgtttggggccagggcaccctggttaccgtgagcagttaatactagagattaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_K1694002_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K1694003_sequence
1
gatattcagatgacacagagcccgagcagcctgagcgcaagcgttggtgatcgcgttaccattacttgttctgccagccaggatatcagcaattatctgaactggtatcagcagaagccgggtaaagccccgaaagtgctgatctacttcaccagcagcctgcatagcggcgttccgagtcgctttagcggtagcggcagcggcaccgatttcaccctgaccattagcagcttacaaccggaagattttgccacctactactgccagcagtatagcaccgtgccgtggacctttggccagggtaccaaagtggagatcaaaggcggtagcagtcgtagcagtagtagtggtggtggtggcagcggtggtggtggtgaagtgcagctggttgaaagcggtggcggtctggttcagccgggtggtagtctgcgcctgagctgtgcagccagcggctatacctttaccaactacggcatgaattgggtgcgtcaggccccgggtaaaggtctggaatgggtgggctggatcaatacctataccggcgaaccgacctacgccgccgactttaaacgccgcttcacctttagcctggacaccagcaaaagcaccgcctatttacaaatgaatagcctgcgcgccgaagataccgcagtgtactactgcgccaagtatccgcactactacggcagcagccattggtactttgacgtttggggccagggcaccctggttaccgtgagcagttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z