LOCUS       BBa_K1694054            2076 bp    DNA     linear       22-Dec-2024
DEFINITION  Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0034+amilCP+B0015
ACCESSION   BBa_K1694054
VERSION     BBa_K1694054.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..501
                     /label=BBa_K1694002
     CDS             510..1244
                     /label=BBa_K1694004
     misc_feature    64..1244
                     /label=BBa_K1694014
     misc_feature    1..1244
                     /label=BBa_K1694024
     misc_feature    1257..1260
                     /label=conserved
     RBS             1253..1264
                     /label=BBa_B0034
     CDS             1271..1936
                     /label=amilCP
     CDS             1271..1939
                     /label=BBa_K592009
     stem_loop       1959..2002
                     /label=stem_loop
     misc_feature    1948..2027
                     /label=BBa_B0010
     terminator      1948..2027
                     /label=BBa_B0010
     stem_loop       2043..2062
                     /label=T7 TE
     misc_feature    2069..2069
                     /label=stop
     polyA_site      2063..2076
                     /label=polya
     misc_feature    2036..2076
                     /label=BBa_B0012
     terminator      2036..2076
                     /label=BBa_B0012
     terminator      1948..2076
                     /label=BBa_B0015
     misc_feature    1253..2076
                     /label=BBa_K1357009
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagaaagcaa ccaagctggt tctgggtgcc gtgattctgg gcagtaccct gttagcaggt
      121 tgttctagca atgccaaaat cgaccaaggc atcaacaaca atggcccgac ccacgaaaac
      181 cagctgggtg ccggtgcctt tggtggttat caggtgaacc cgtacgtggg ctttgaaatg
      241 ggctatgatt ggctgggccg catgccgtac aaaggcagtg tggagaacgg cgcctataaa
      301 gcacagggcg tgcagctgac agcaaaactg ggctacccta ttaccgacga cctggacatc
      361 tacacacgct taggcggcat ggtgtggcgc gccgatacca agagcaacgt gtacggcaag
      421 aaccacgata ccggcgtgag tccggtgttt gccggcggtg tggagtatgc aatcaccccg
      481 gaaattgcca cacgtccatg gtactagagg atattctgct gacccagagc ccggtgatcc
      541 tgagcgtgag tccgggtgaa cgtgtgagct tcagttgccg tgccagccag agcatcggta
      601 ccaacatcca ctggtatcag cagcgcacca acggtagtcc gcgcctgctg atcaaatatg
      661 ccagcgagag catcagcggc atcccgagcc gctttagtgg tagcggcagc ggcaccgatt
      721 ttaccctgag catcaacagc gtggaaagcg aagacatcgc agattattat tgccagcaga
      781 acaataattg gccgaccacc ttcggcgccg gtaccaaact ggaactgaaa ggcggtagca
      841 gccgtagtag cagcagtggt ggtggtggta gtggtggcgg tggccaggtt cagctgaaac
      901 agagcggtcc gggtctggtg cagcctagcc agagcctgag cattacctgc accgtgagcg
      961 gctttagcct gaccaattac ggcgtgcatt gggtgcgtca gagcccgggc aagggtctgg
     1021 aatggctggg tgttatctgg agcggcggca ataccgatta caacaccccg ttcaccagcc
     1081 gcctgagtat taacaaagat aatagcaaaa gccaggtctt ttttaaaatg aatagcttac
     1141 agagcaatga caccgcaatt tattactgcg cccgcgccct gacctactat gattatgagt
     1201 tcgcatactg gggtcagggc accctggtga cagtgagtgc ataatactag agaaagagga
     1261 gaaatactag atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac
     1321 ggtcaatgga cactactttg aggtcgaagg cgatggaaaa ggtaagccct acgaggggga
     1381 gcagacggta aagctcactg tcaccaaggg cggacctctg ccatttgctt gggatatttt
     1441 atcaccacag tgtcagtacg gaagcatacc attcaccaag taccctgaag acatccctga
     1501 ctatgtaaag cagtcattcc cggagggcta tacatgggag aggatcatga actttgaaga
     1561 tggtgcagtg tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacca
     1621 tgtcaagttc tctggtttga actttcctcc caatggacct gtcatgcaga agaagacaca
     1681 gggctgggaa cccaacactg agcgtctctt tgcacgagat ggaatgctgc taggaaacaa
     1741 ctttatggct ctgaagttag aaggaggcgg tcactatttg tgtgaattta aaactactta
     1801 caaggcaaag aagcctgtga agatgccagg gtatcactat gttgaccgca aactggatgt
     1861 aaccaatcac aacaaggatt acacttcggt tgagcagtgt gaaatttcca ttgcacgcaa
     1921 acctgtggtc gcctaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1981 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2041 ctggctcacc ttcgggtggg cctttctgcg tttata
//