BBa_K1694054
type: ComponentDefinition
persistent identity: BBa K1694054
display id: BBa_K1694054
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0034+amilCP+B0015
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T10:07:22Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: D
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27160
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1694024
component: BBa_K1357009
sequence annotation: BBa_K1694024
sequence annotation: BBa_K1357009
sequence: https://synbiohub.org/public/igem/BBa_K1694054_sequence/1

BBa_K1694024
type: Component
persistent identity: component2457678
display id: component2457678
version: 1
top level: BBa_K1694054
definition: BBa_K1694024
access: public

BBa_K1357009
type: Component
persistent identity: component2457690
display id: component2457690
version: 1
top level: BBa_K1694054
definition: BBa_K1357009
access: public

BBa_K1694024
type: SequenceAnnotation
persistent identity: annotation2457678
display id: annotation2457678
version: 1
top level: BBa_K1694054
location: https://synbiohub.org/public/igem/BBa_K1694054/annotation2457678/range2457678/1
component: BBa_K1694024

https://synbiohub.org/public/igem/BBa_K1694054/annotation2457678/range2457678/1
type: Range
persistent identity: range2457678
display id: range2457678
version: 1
top level: BBa_K1694054
direction: direction/reverse
start: 1
end: 1244
orientation: inline

BBa_K1357009
type: SequenceAnnotation
persistent identity: annotation2457690
display id: annotation2457690
version: 1
top level: BBa_K1694054
location: https://synbiohub.org/public/igem/BBa_K1694054/annotation2457690/range2457690/1
component: BBa_K1357009

https://synbiohub.org/public/igem/BBa_K1694054/annotation2457690/range2457690/1
type: Range
persistent identity: range2457690
display id: range2457690
version: 1
top level: BBa_K1694054
direction: direction/reverse
start: 1253
end: 2076
orientation: inline

BBa_K1694024
type: ComponentDefinition
persistent identity: BBa K1694024
display id: BBa_K1694024
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T08:07:32Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Cetuximab) outside the E.coli cell membrane.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 28523
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23101
component: BBa_K1694014
component: BBa_B0034
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_K1694014
sequence: https://synbiohub.org/public/igem/BBa_K1694024_sequence/1

BBa_J23101
type: Component
persistent identity: component2457382
display id: component2457382
version: 1
top level: BBa_K1694024
definition: BBa_J23101
access: public

BBa_K1694014
type: Component
persistent identity: component2457387
display id: component2457387
version: 1
top level: BBa_K1694024
definition: BBa_K1694014
access: public

BBa_B0034
type: Component
persistent identity: component2457384
display id: component2457384
version: 1
top level: BBa_K1694024
definition: BBa_B0034
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2457382
display id: annotation2457382
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457382/range2457382/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457382/range2457382/1
type: Range
persistent identity: range2457382
display id: range2457382
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2457384
display id: annotation2457384
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457384/range2457384/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457384/range2457384/1
type: Range
persistent identity: range2457384
display id: range2457384
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1694014
type: SequenceAnnotation
persistent identity: annotation2457387
display id: annotation2457387
version: 1
top level: BBa_K1694024
location: https://synbiohub.org/public/igem/BBa_K1694024/annotation2457387/range2457387/1
component: BBa_K1694014

https://synbiohub.org/public/igem/BBa_K1694024/annotation2457387/range2457387/1
type: Range
persistent identity: range2457387
display id: range2457387
version: 1
top level: BBa_K1694024
direction: direction/reverse
start: 64
end: 1244
orientation: inline

BBa_K1357009
type: ComponentDefinition
persistent identity: BBa K1357009
display id: BBa_K1357009
version: 1
was generated by: iGEM to SBOL conversion
description: amilCP Reporter (RBS+Coding+Term)
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
mutable provenance: jellyfish
owned by: james
owned by: myers
mutable description: Show functionality of gene circuit
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Matthew Tucker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K592009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K592009
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1357009_sequence/1

BBa_B0015
type: Component
persistent identity: component2408711
display id: component2408711
version: 1
top level: BBa_K1357009
definition: BBa_B0015
access: public

BBa_K592009
type: Component
persistent identity: component2408704
display id: component2408704
version: 1
top level: BBa_K1357009
definition: amilCP
access: public

BBa_B0034
type: Component
persistent identity: component2408702
display id: component2408702
version: 1
top level: BBa_K1357009
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408702
display id: annotation2408702
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
type: Range
persistent identity: range2408702
display id: range2408702
version: 1
top level: BBa_K1357009
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2408704
display id: annotation2408704
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
type: Range
persistent identity: range2408704
display id: range2408704
version: 1
top level: BBa_K1357009
direction: direction/reverse
start: 19
end: 687
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408711
display id: annotation2408711
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
type: Range
persistent identity: range2408711
display id: range2408711
version: 1
top level: BBa_K1357009
direction: direction/reverse
start: 696
end: 824
orientation: inline

BBa_K1694014
type: ComponentDefinition
persistent identity: BBa K1694014
display id: BBa_K1694014
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA-scFv(Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T09:37:51Z
mutable provenance: Assemble
owned by: james
owned by: myers
mutable description: We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27153
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1694004
component: BBa_K1694002
sequence annotation: BBa_K1694002
sequence annotation: BBa_K1694004
sequence: https://synbiohub.org/public/igem/BBa_K1694014_sequence/1

BBa_K1694004
type: Component
persistent identity: component2457314
display id: component2457314
version: 1
top level: BBa_K1694014
definition: BBa_K1694004
access: public

BBa_K1694002
type: Component
persistent identity: component2457313
display id: component2457313
version: 1
top level: BBa_K1694014
definition: BBa_K1694002
access: public

BBa_K1694002
type: SequenceAnnotation
persistent identity: annotation2457313
display id: annotation2457313
version: 1
top level: BBa_K1694014
location: https://synbiohub.org/public/igem/BBa_K1694014/annotation2457313/range2457313/1
component: BBa_K1694002

https://synbiohub.org/public/igem/BBa_K1694014/annotation2457313/range2457313/1
type: Range
persistent identity: range2457313
display id: range2457313
version: 1
top level: BBa_K1694014
direction: direction/reverse
start: 1
end: 438
orientation: inline

BBa_K1694004
type: SequenceAnnotation
persistent identity: annotation2457314
display id: annotation2457314
version: 1
top level: BBa_K1694014
location: https://synbiohub.org/public/igem/BBa_K1694014/annotation2457314/range2457314/1
component: BBa_K1694004

https://synbiohub.org/public/igem/BBa_K1694014/annotation2457314/range2457314/1
type: Range
persistent identity: range2457314
display id: range2457314
version: 1
top level: BBa_K1694014
direction: direction/reverse
start: 447
end: 1181
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

amilCP
type: ComponentDefinition
persistent identity: BBa K592009
display id: BBa_K592009
version: 1
was generated by: iGEM to SBOL conversion
description: amilCP, blue chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Acropora millepora
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: amilCP
sequence: https://synbiohub.org/public/igem/BBa_K592009_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2131628
display id: annotation2131628
version: 1
top level: amilCP
location: https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
type: Range
persistent identity: range2131628
display id: range2131628
version: 1
top level: amilCP
direction: direction/forward
start: 1
end: 666
orientation: inline

BBa_K1694002
type: ComponentDefinition
persistent identity: BBa K1694002
display id: BBa_K1694002
version: 1
was generated by: iGEM to SBOL conversion
description: Lpp-OmpA-N
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T09:58:58Z
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
mutable description: To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 27160
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1694002_sequence/1

BBa_K1694004
type: ComponentDefinition
persistent identity: BBa K1694004
display id: BBa_K1694004
version: 1
was generated by: iGEM to SBOL conversion
description: Single-chain variable fragment (Anti-EGFR)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T07:29:10Z
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
mutable description: We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 28523
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1694004_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1694054_sequence/1
type: Sequence
persistent identity: BBa K1694054 sequence
display id: BBa_K1694054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataatactagagaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694024_sequence/1
type: Sequence
persistent identity: BBa K1694024 sequence
display id: BBa_K1694024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1357009_sequence/1
type: Sequence
persistent identity: BBa K1357009 sequence
display id: BBa_K1357009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694014_sequence/1
type: Sequence
persistent identity: BBa K1694014 sequence
display id: BBa_K1694014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592009_sequence/1
type: Sequence
persistent identity: BBa K592009 sequence
display id: BBa_K592009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694002_sequence/1
type: Sequence
persistent identity: BBa K1694002 sequence
display id: BBa_K1694002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694004_sequence/1
type: Sequence
persistent identity: BBa K1694004 sequence
display id: BBa_K1694004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z