BBa_K1694005 1 BBa_K1694005 Single-chain variable fragment (Anti-HER2) 2015-09-14T11:00:00Z 2015-09-18T07:36:18Z Artificial synthesis We selected the single chain variable fragments (scFv) of monoclonal antibodies Trastuzumab false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1357009 1 BBa_K1357009 amilCP Reporter (RBS+Coding+Term) 2014-10-08T11:00:00Z 2015-05-08T01:10:02Z jellyfish Show functionality of gene circuit false false _1733_ 0 16772 9 In stock false None false Matthew Tucker component2408711 1 BBa_B0015 component2408704 1 BBa_K592009 component2408702 1 BBa_B0034 annotation2408704 1 BBa_K592009 range2408704 1 19 687 annotation2408711 1 BBa_B0015 range2408711 1 696 824 annotation2408702 1 BBa_B0034 range2408702 1 1 12 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1694055 1 BBa_K1694055 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)+B0034+amilCP+B0015 2015-09-14T11:00:00Z 2015-09-20T11:44:12Z Assembly C false false _2112_ 22408 28523 9 false No false CHIH-HSUAN HSU component2457625 1 BBa_K1694025 component2457637 1 BBa_K1357009 annotation2457625 1 BBa_K1694025 range2457625 1 1 1089 annotation2457637 1 BBa_K1357009 range2457637 1 1098 1921 BBa_K1694002 1 BBa_K1694002 Lpp-OmpA-N 2015-09-14T11:00:00Z 2015-09-18T09:58:58Z Artificial synthesis To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU BBa_K592009 1 amilCP amilCP, blue chromoprotein 2011-09-17T11:00:00Z 2015-05-08T01:12:48Z Acropora millepora Released HQ 2013 This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI). false false _763_ 0 7929 9 In stock true Illegal internal restriction site had to be removed (EcoRI). false Lei Sun annotation2131628 1 amilCP range2131628 1 1 666 BBa_K1694025 1 BBa_K1694025 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2) 2015-09-14T11:00:00Z 2015-09-18T09:01:40Z Assembly By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Trastuzumab) outside the E.coli cell membrane. false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU component2457401 1 BBa_K1694015 component2457398 1 BBa_B0034 component2457396 1 BBa_J23101 annotation2457398 1 BBa_B0034 range2457398 1 44 55 annotation2457401 1 BBa_K1694015 range2457401 1 64 1089 annotation2457396 1 BBa_J23101 range2457396 1 1 35 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1694015 1 BBa_K1694015 OmpA-scFv(Anti-HER2) 2015-09-14T11:00:00Z 2015-09-18T08:50:13Z Assemble We selected the single chain variable fragments (scFv) of monoclonal antibodies Trastuzumab false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU component2457347 1 BBa_K1694005 component2457346 1 BBa_K1694002 annotation2457346 1 BBa_K1694002 range2457346 1 1 438 annotation2457347 1 BBa_K1694005 range2457347 1 445 1026 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1694005_sequence 1 atgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt BBa_K1694015_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt BBa_K592009_sequence 1 atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa BBa_K1694002_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg BBa_K1694055_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgttactagagaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1357009_sequence 1 aaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1694025_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z