BBa_K1695029 1 BBa_K1695029 RBS + Bacteriophage λ codon-optimized S gene 2015-08-11T11:00:00Z 2015-09-17T01:51:59Z Bacteriophage Lambda Bacteriophage Lambda Holin Codon Optimized with Riboswitch false false _2113_ 26466 26466 9 false with Riboswitch false Aruna Surendran Ramkrishnan annotation2435109 1 BBa_B0034 range2435109 1 1 12 annotation2435108 1 conserved range2435108 1 5 8 annotation2435110 1 BBa_K1695015 range2435110 1 19 336 BBa_K1695039 1 BBa_K1695039 RBS Bacteriophage λ Codon Optimized Lysis Cassette S R Rz 2015-08-12T11:00:00Z 2015-09-17T06:50:17Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Holin, Endolysin, Spanin codon optimized with strong promoters to lyse the cells. false false _2113_ 26466 26585 9 false no false Ma Lee component2437508 1 BBa_K1695033 component2437500 1 BBa_K1695029 component2437504 1 BBa_K1695032 annotation2437504 1 BBa_K1695032 range2437504 1 345 839 annotation2437508 1 BBa_K1695033 range2437508 1 848 1327 annotation2437500 1 BBa_K1695029 range2437500 1 1 336 BBa_K1695032 1 BBa_K1695032 RBS + Bacteriophage λ codon-optimized R gene 2015-08-11T11:00:00Z 2015-09-17T05:43:33Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Endolysin codon optimized with strong promoter to lyse the cell wall. false false _2113_ 26466 26585 9 false no. false Ma Lee annotation2435115 1 BBa_B0034 range2435115 1 1 12 annotation2435114 1 conserved range2435114 1 5 8 annotation2435116 1 BBa_K1695016 range2435116 1 19 495 BBa_K1695000 1 BBa_K1695000 Tightly-regulated lactose-inducible promoter 2015-08-09T11:00:00Z 2015-09-18T03:44:47Z Synthesized gene Repressor Gene For Lysis Casette false false _2113_ 26466 26466 9 false Repressor Gene For Lysis Casette false Aruna Surendran Ramkrishnan BBa_K1695033 1 BBa_K1695033 RBS + Bacteriophage λ codon optimized Rz gene 2015-08-11T11:00:00Z 2015-09-17T05:46:50Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Spanin codon optimized with strong promoter to lyse the cell membrane. false false _2113_ 26466 26585 9 false no. false Ma Lee annotation2435119 1 BBa_K1695019 range2435119 1 19 480 annotation2435118 1 BBa_B0034 range2435118 1 1 12 annotation2435117 1 conserved range2435117 1 5 8 BBa_K1695044 1 BBa_K1695044 pLacIQ + RBS Bacteriophage λ Codon Optimized Lysis Cassette S R Rz 2015-08-16T11:00:00Z 2015-09-11T01:42:24Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Holin, Endolysin, Spanin codon optimized with strong promoters to lyse the cells and lacI gene to repress the lysis cassette when the lactose is absent. false false _2113_ 26466 26585 9 false no false Ma Lee component2437612 1 BBa_K1695000 component2437625 1 BBa_K1695039 annotation2437625 1 BBa_K1695039 range2437625 1 1279 2605 annotation2437612 1 BBa_K1695000 range2437612 1 1 1270 BBa_K1695033_sequence 1 aaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695032_sequence 1 aaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaa BBa_K1695000_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatt BBa_K1695039_sequence 1 aaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtgcaattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaatactagagaaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaatactagagaaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695044_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatttactagagaaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtgcaattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaatactagagaaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaatactagagaaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695029_sequence 1 aaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtgcaattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z