BBa_K1695032 1 BBa_K1695032 RBS + Bacteriophage λ codon-optimized R gene 2015-08-11T11:00:00Z 2015-09-17T05:43:33Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Endolysin codon optimized with strong promoter to lyse the cell wall. false false _2113_ 26466 26585 9 false no. false Ma Lee annotation2435115 1 BBa_B0034 range2435115 1 1 12 annotation2435114 1 conserved range2435114 1 5 8 annotation2435116 1 BBa_K1695016 range2435116 1 19 495 BBa_K1695045 1 BBa_K1695045 pL8-UV5 + RBS Bacteriophage λ Codon Optimized Lysis Cassette S mutant R Rz 2015-08-16T11:00:00Z 2015-09-17T07:04:36Z Bacteriophage Lambda Bacteriophage Lambda mutant Holin codon optimized,wild type Endolysin and Spanin original with strong promoters to lyse the cells and a lacI gene to repress the lysis cassette when the lactose is absent. false false _2113_ 26466 26585 9 false no false Ma Lee component2437626 1 BBa_K1695000 component2437639 1 BBa_K1695038 annotation2437626 1 BBa_K1695000 range2437626 1 1 1270 annotation2437639 1 BBa_K1695038 range2437639 1 1279 2605 BBa_K1695033 1 BBa_K1695033 RBS + Bacteriophage λ codon optimized Rz gene 2015-08-11T11:00:00Z 2015-09-17T05:46:50Z Bacteriophage Lambda Bacteriophage Lambda Wild-type Spanin codon optimized with strong promoter to lyse the cell membrane. false false _2113_ 26466 26585 9 false no. false Ma Lee annotation2435119 1 BBa_K1695019 range2435119 1 19 480 annotation2435117 1 conserved range2435117 1 5 8 annotation2435118 1 BBa_B0034 range2435118 1 1 12 BBa_K1695000 1 BBa_K1695000 Tightly-regulated lactose-inducible promoter 2015-08-09T11:00:00Z 2015-09-18T03:44:47Z Synthesized gene Repressor Gene For Lysis Casette false false _2113_ 26466 26466 9 false Repressor Gene For Lysis Casette false Aruna Surendran Ramkrishnan BBa_K1695038 1 BBa_K1695038 RBS Bacteriophage λ Codon Optimized Lysis Cassette S mutant R Rz 2015-08-12T11:00:00Z 2015-09-17T06:51:56Z Bacteriophage Lambda Bacteriophage Lambda mutant Holin, wild type Endolysin, wild type Spanin codon optimized with strong promoters to lyse the cells. false false _2113_ 26466 26585 9 false no false Ma Lee component2437512 1 BBa_K1695030 component2437520 1 BBa_K1695033 component2437516 1 BBa_K1695032 annotation2437516 1 BBa_K1695032 range2437516 1 345 839 annotation2437512 1 BBa_K1695030 range2437512 1 1 336 annotation2437520 1 BBa_K1695033 range2437520 1 848 1327 BBa_K1695030 1 BBa_K1695030 RBS + Bacteriophage λ codon-optimized S mutant gene 2015-08-11T11:00:00Z 2015-09-17T05:37:34Z Bacteriophage Lambda Bacteriophage Lambda Mutant S Codon Optimized with Strong RBS false false _2113_ 26466 26466 9 false Target mutation: Alanine at position 50 (S105) to glycine (GGT) false Aruna Surendran Ramkrishnan annotation2435107 1 BBa_K1695021 range2435107 1 19 336 annotation2435105 1 conserved range2435105 1 5 8 annotation2435106 1 BBa_B0034 range2435106 1 1 12 BBa_K1695033_sequence 1 aaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695032_sequence 1 aaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaa BBa_K1695045_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatttactagagaaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtggtattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaatactagagaaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaatactagagaaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695000_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatt BBa_K1695030_sequence 1 aaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtggtattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaa BBa_K1695038_sequence 1 aaagaggagaaatactagatgccggaaaaacatgatctgctggcagcaattctggcagccaaagaacagggtattggtgccattctggcatttgcaatggcatatctgcgtggtcgttataatggtggtgcatttaccaaaaccgttattgatgcaaccatgtgtggtattattgcctggtttattcgtgacctgctggattttgcaggtctgtcaagcaatctggcatatatcaccagcgtttttattggttatatcggcaccgatagcattggcagcctgattaaacgttttgcagcaaaaaaagccggtgttgaagatggtcgtaatcagtaatactagagaaagaggagaaatactagatggtcgaaattaacaatcagcgcaaagcctttctggatatgctggcttggagcgaaggcaccgataatggtcgtcagaaaacccgtaatcatggctatgatgttattgttggtggtgagctgtttaccgattattcagatcatccgcgtaaactggttaccctgaatccgaaactgaaaagtaccggtgcaggtcgttatcagctgctgtcacgttggtgggatgcatatcgcaaacagctgggtctgaaagattttagcccgaaaagccaggatgcagttgcactgcaacaaattaaagaacgtggtgcactgccgatgattgatcgtggtgatattcgtcaggcaattgatcgttgtagcaatatttgggcaagcctgcctggtgcaggttatggtcagtttgaacataaagcagatagcctgatcgccaaattcaaagaagcaggcggtacagttcgtgaaattgatgtttaatactagagaaagaggagaaatactagatgagccgtgttaccgcaattattagcgcactggttatttgcattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcccgtgaactgaaactggcaaatgcagcaattaccgatatgcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcatcaccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z