BBa_K1695000 1 BBa_K1695000 Tightly-regulated lactose-inducible promoter 2015-08-09T11:00:00Z 2015-09-18T03:44:47Z Synthesized gene Repressor Gene For Lysis Casette false false _2113_ 26466 26466 9 false Repressor Gene For Lysis Casette false Aruna Surendran Ramkrishnan BBa_K1695041 1 BBa_K1695041 RBS Bacteriophage 21 Codon Optimized Lysis Cassette S mutant R Rz 2015-08-16T11:00:00Z 2015-09-17T06:57:11Z E.coli RBS_S_mutant_21_co + RBS_R_21_co + RBS_Rz_21_co false false _2113_ 26466 26466 9 It's complicated false With RBS for great expression false Aruna Surendran Ramkrishnan component2437568 1 BBa_K1695010 component2437572 1 BBa_K1695004 component2437576 1 BBa_K1695006 annotation2437568 1 BBa_K1695010 range2437568 1 1 153 annotation2437576 1 BBa_K1695006 range2437576 1 686 1165 annotation2437572 1 BBa_K1695004 range2437572 1 162 677 BBa_K1695010 1 BBa_K1695010 RBS + Bacteriophage 21 S mutant gene 2015-08-10T11:00:00Z 2015-09-17T06:22:18Z Bacteriophage 21 Bacteriophage 21 Wild Type Mutant Holin with Strong RBS false false _2113_ 26466 26466 9 false Deletion of TMD1 & serine at position 41 (S68) to asparagine Addition of Strong RBS false Aruna Surendran Ramkrishnan annotation2435038 1 BBa_B0034 range2435038 1 1 12 annotation2435037 1 conserved range2435037 1 5 8 annotation2435039 1 BBa_K1695009 range2435039 1 19 153 BBa_K1695048 1 BBa_K1695048 pL8-UV5 + RBS Bacteriophage 21 Codon Optimized Lysis Cassette S mutant R Rz 2015-08-16T11:00:00Z 2015-09-17T07:12:59Z E.coli PlacI_iG15 + RBS_Sm_Lysis_21_co false false _2113_ 26466 26466 9 false With LacI strong promoter false Aruna Surendran Ramkrishnan component2437666 1 BBa_K1695000 component2437679 1 BBa_K1695041 annotation2437679 1 BBa_K1695041 range2437679 1 1279 2443 annotation2437666 1 BBa_K1695000 range2437666 1 1 1270 BBa_K1695006 1 BBa_K1695006 RBS + Bacteriophage 21 codon optimized Rz gene 2015-08-10T11:00:00Z 2015-09-17T06:32:31Z Bacteriphage 21 Bacteriophage 21 Spannin codon optimized with strong RBS false false _2113_ 26466 26466 9 false Strong RBS false Aruna Surendran Ramkrishnan annotation2435033 1 BBa_K1695005 range2435033 1 19 480 annotation2435032 1 BBa_B0034 range2435032 1 1 12 annotation2435031 1 conserved range2435031 1 5 8 BBa_K1695004 1 BBa_K1695004 RBS + Bacteriophage 21 codon optimized R gene 2015-08-10T11:00:00Z 2015-09-17T06:30:37Z Bacteriophage 21 Bacteriophage 21 endolysin codon optimized with strong promoter false false _2113_ 26466 26466 9 false Strong promoter added false Aruna Surendran Ramkrishnan annotation2435029 1 BBa_B0034 range2435029 1 1 12 annotation2435028 1 conserved range2435028 1 5 8 annotation2435030 1 BBa_K1695003 range2435030 1 19 516 BBa_K1695006_sequence 1 aaagaggagaaatactagatgaatcgtgtgaccgcaattattagcgcactggtgatttgtattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcacgtgaactgaccctggcaaatcgtgttattaccgatattcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcaagtccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695010_sequence 1 aaagaggagaaatactagatggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaaaccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataa BBa_K1695000_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatt BBa_K1695048_sequence 1 cgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcattcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtgaaattgtgagcggataacaatttactagagaaagaggagaaatactagatggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaaaccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatactagagaaagaggagaaatactagatgcctccgagcctgcgtaaagcagttgcagcagcaattggtggtggtgcaattgccattgcaagcgttctgattaccggtccgagcggtaatgatggtctggaaggtgttagctatatcccgtataaagatattgttggtgtttggaccgtttgccatggtcataccggcaaagatattatgctgggtaaaacctataccaaagccgaatgtaaagccctgctgaataaagatctggcaaccgttgcacgtcagattaatccgtatatcaaagtggatattccggaaaccatgcgtggtgcactgtatagcctgctgtataatgttggtgcaggtaattttcgtaccagcaccctgctgcgtaaaatcaatcagggtgatattaaaggtgcatgtgatcagctgcgtcgttggacctatgccggtggtaaacagtggaaaggtctgatgacccgtcgtgaaattgaacgtgaaatttgtctgtggggtcagcagtaatactagagaaagaggagaaatactagatgaatcgtgtgaccgcaattattagcgcactggtgatttgtattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcacgtgaactgaccctggcaaatcgtgttattaccgatattcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcaagtccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa BBa_K1695004_sequence 1 aaagaggagaaatactagatgcctccgagcctgcgtaaagcagttgcagcagcaattggtggtggtgcaattgccattgcaagcgttctgattaccggtccgagcggtaatgatggtctggaaggtgttagctatatcccgtataaagatattgttggtgtttggaccgtttgccatggtcataccggcaaagatattatgctgggtaaaacctataccaaagccgaatgtaaagccctgctgaataaagatctggcaaccgttgcacgtcagattaatccgtatatcaaagtggatattccggaaaccatgcgtggtgcactgtatagcctgctgtataatgttggtgcaggtaattttcgtaccagcaccctgctgcgtaaaatcaatcagggtgatattaaaggtgcatgtgatcagctgcgtcgttggacctatgccggtggtaaacagtggaaaggtctgatgacccgtcgtgaaattgaacgtgaaatttgtctgtggggtcagcagtaa BBa_K1695041_sequence 1 aaagaggagaaatactagatggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaaaccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatactagagaaagaggagaaatactagatgcctccgagcctgcgtaaagcagttgcagcagcaattggtggtggtgcaattgccattgcaagcgttctgattaccggtccgagcggtaatgatggtctggaaggtgttagctatatcccgtataaagatattgttggtgtttggaccgtttgccatggtcataccggcaaagatattatgctgggtaaaacctataccaaagccgaatgtaaagccctgctgaataaagatctggcaaccgttgcacgtcagattaatccgtatatcaaagtggatattccggaaaccatgcgtggtgcactgtatagcctgctgtataatgttggtgcaggtaattttcgtaccagcaccctgctgcgtaaaatcaatcagggtgatattaaaggtgcatgtgatcagctgcgtcgttggacctatgccggtggtaaacagtggaaaggtctgatgacccgtcgtgaaattgaacgtgaaatttgtctgtggggtcagcagtaatactagagaaagaggagaaatactagatgaatcgtgtgaccgcaattattagcgcactggtgatttgtattattgtgtgtctgtcatgggcagtgaatcattatcgtgataatgccattacctataaagcccagcgtgataaaaatgcacgtgaactgaccctggcaaatcgtgttattaccgatattcagatgcgtcagcgtgatgttgcagcactggatgcaaaatataccaaagaactggcagatgccaaagcagaaaatgatgcactgcgtgatgatgttgccgcaggtcgtcgtcgtctgcatattaaagcagtttgtcagagcgttcgtgaagcaaccaccgcaagcggtgttgataatgcagcaagtccgcgtctggcagataccgcagaacgtgattatttcaccctgcgtgaacgtctgattaccatgcagaaacagctggaaggcacccagaaatacattaatgaacagtgccgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z