LOCUS       BBa_K1695053            2153 bp    DNA     linear       27-Dec-2024
DEFINITION  Tightly-regulated lactose-inducible promoter with GFP
ACCESSION   BBa_K1695053
VERSION     BBa_K1695053.1  
FEATURES             Location/Qualifiers
     promoter        1..1270
                     /label=BBa_K1695000
     misc_feature    1283..1286
                     /label=conserved
     RBS             1279..1290
                     /label=BBa_B0034
     CDS             1297..2016
                     /label=GFP protein
     CDS             1297..2016
                     /label=BBa_E0040
     stem_loop       2036..2079
                     /label=stem_loop
     misc_feature    2025..2104
                     /label=BBa_B0010
     terminator      2025..2104
                     /label=BBa_B0010
     stem_loop       2120..2139
                     /label=T7 TE
     misc_feature    2146..2146
                     /label=stop
     polyA_site      2140..2153
                     /label=polya
     misc_feature    2113..2153
                     /label=BBa_B0012
     terminator      2113..2153
                     /label=BBa_B0012
     misc_feature    1279..2153
                     /label=BBa_I13504
ORIGIN
        1 cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
       61 gagtcaattc agggtggtga atatgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcattcatt
     1201 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgaa attgtgagcg
     1261 gataacaatt tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1321 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1381 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1441 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1501 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1561 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1621 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1681 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1741 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1801 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1861 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1921 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1981 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     2041 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2101 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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