LOCUS BBa_K1695055 4415 bp DNA linear 25-Nov-2024 DEFINITION Strong Promoter LacZY ACCESSION BBa_K1695055 VERSION BBa_K1695055.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..54 /label=RBS-4\Weaker misc_feature 44..54 /label=BBa_B0033 RBS 50..53 /label=RBS misc_feature 61..1314 /label=BBa_K1695023 misc_feature 44..1314 /label=BBa_K1695054 misc_feature 1327..1330 /label=conserved RBS 1323..1334 /label=BBa_B0034 misc_feature 4364..4364 /label=C->T CDS 1341..4415 /label=lacZ misc_feature 1341..1343 /label=START misc_feature 4413..4415 /label=STOP CDS 1341..4415 /label=BBa_I732005 misc_feature 1323..4415 /label=BBa_I732017 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag 61 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 121 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 181 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 241 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 301 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 361 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 421 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 481 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 541 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 601 gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg 661 gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca 721 aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac 781 caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta 841 tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca 901 ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct 961 gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg 1021 ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag 1081 tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg 1141 gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc 1201 gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt 1261 agcggccccg gtccgctttc tctactgcgt cgtcaggtga atgaagtcgc ttaatactag 1321 agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc gttttacaac 1381 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 1441 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 1501 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct 1561 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc 1621 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt 1681 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct 1741 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt 1801 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc 1861 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg 1921 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct 1981 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg 2041 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact 2101 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc 2161 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc 2221 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg 2281 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc 2341 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag 2401 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg 2461 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc 2521 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag 2581 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc 2641 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg 2701 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc 2761 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca 2821 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc 2881 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc 2941 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact 3001 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc 3061 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg 3121 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc 3181 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg 3241 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc 3301 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg 3361 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc 3421 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc 3481 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg 3541 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta 3601 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata 3661 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca 3721 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg 3781 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg 3841 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc 3901 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg 3961 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag 4021 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg 4081 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga 4141 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg 4201 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc 4261 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga 4321 ttggtggcga cgactcctgg agcccgtcag tatcggcgga atttcagctg agcgccggtc 4381 gctaccatta ccagttggtc tggtgtcaaa aataa //