BBa_K1696006 1 BBa_K1696006 P170-RFP 2015-09-10T11:00:00Z 2015-09-15T09:05:47Z Based on BBa_K1696005, P170 promoter. We add RFP reporter gene after P170 promoter. First, we want to test whether the inducible promoter is leaky or not. Then we use RFP gene to characterize the lactate-sensing system's performance in E.coli. false false _2114_ 16881 16881 9 false We choose a strong RBS. false Yue Liu annotation2449415 1 P170 promoter range2449415 1 1 418 annotation2449419 1 B0014 range2449419 1 1131 1226 annotation2449416 1 B0034 range2449416 1 427 438 annotation2449418 1 RFP range2449418 1 455 1122 BBa_K1696006_sequence 1 ggaagtaattgtagagctttgcatgtctataaaaaaatgggattccaaattaccgatatcaatatgcgaaaagaactatgaatatccactccatttttggttgccatttgttaacgctgcctcctctccctagtgctataataaaaatggccaaaaaaaaaccattttattgactatatttgcaatttatttacacattatcttttcagaaccaaaatctggcccattttggaacagacttctactattttgttgtctagtaggagcttttttgttttgggcaacttgttttcttcgtaatttctgtcagtggctgatgattaaatgttttacttttcattgctctaatcagcttctcataggcaaatcaaataaagtctgatataacactcaatagcaaacatcggaggtagatagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaactccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z