BBa_K143021 1 RBS-spoVG SpoVG ribosome binding site (RBS) for B. subtilis 2008-09-16T11:00:00Z 2015-05-08T01:10:23Z The sequence was taken from a previous research paper [1] and was constructed by Geneart. Released HQ 2013 Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite> false true _199_ 0 2090 9 In stock false In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained. false James Chappell annotation1975997 1 rbs range1975997 1 1 12 BBa_K1697001 1 <partinfo> moved to <partinfo>BBa_K1697002 </partinfo> 2015-09-13T11:00:00Z 2015-09-18T04:10:33Z Promoter RBS this part was designed with gram positive bacteria in mind. However, we are unable to characterize it in gram positive organism in E. coli true false _2115_ 24798 24795 9 false all the promoter and RBS are compatible with gram positive organism false UI Indonesia 2015 component2454755 1 BBa_K143021 annotation2454755 1 BBa_K143021 range2454755 1 1 12 BBa_K143021_sequence 1 aaaggtggtgaa BBa_K1697001_sequence 1 aaaggtggtgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z