BBa_K1697002
type: ComponentDefinition
persistent identity: BBa K1697002
display id: BBa_K1697002
version: 1
was generated by: iGEM to SBOL conversion
description: Toggle switch for gram positive
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T09:43:24Z
mutable provenance: DNA synthesis
owned by: james
owned by: myers
mutable description: this part was designed with gram positive bacteria in mind. However, we are unable to characterize it in gram positive organism in E. coli
discontinued: false
dominant: false
experience: experience/Works
group u list: _2115_
m user id: 24798
owner id: 24798
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We have to find a promoter that can be reversibly activated by a protein
star: false
creator: UI Indonesia 2015
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_K143021
component: BBa_K143015
component: BBa_B0015
component: BBa_K143036
component: BBa_E1010
component: BBa_K143014
component: BBa_K143033
sequence annotation: BBa_K143014
sequence annotation: BBa_K143021
sequence annotation: BBa_K143033
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K143015
sequence annotation: BBa_K143036
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1697002_sequence/1

BBa_E0040
type: Component
persistent identity: component2475118
display id: component2475118
version: 1
top level: BBa_K1697002
definition: GFP
access: public

BBa_K143021
type: Component
persistent identity: component2475116
display id: component2475116
version: 1
top level: BBa_K1697002
definition: RBS-spoVG
access: public

BBa_K143015
type: Component
persistent identity: component2475130
display id: component2475130
version: 1
top level: BBa_K1697002
definition: Ph-s
access: public

BBa_B0015
type: Component
persistent identity: component2475125
display id: component2475125
version: 1
top level: BBa_K1697002
definition: BBa_B0015
access: public

BBa_K143036
type: Component
persistent identity: component2475137
display id: component2475137
version: 1
top level: BBa_K1697002
definition: XylR
access: public

BBa_K143021
type: Component
persistent identity: component2475132
display id: component2475132
version: 1
top level: BBa_K1697002
definition: RBS-spoVG
access: public

BBa_E1010
type: Component
persistent identity: component2475142
display id: component2475142
version: 1
top level: BBa_K1697002
definition: mRFP1
access: public

BBa_K143014
type: Component
persistent identity: component2475107
display id: component2475107
version: 1
top level: BBa_K1697002
definition: Pxyl
access: public

BBa_K143021
type: Component
persistent identity: component2475139
display id: component2475139
version: 1
top level: BBa_K1697002
definition: RBS-spoVG
access: public

BBa_K143033
type: Component
persistent identity: component2475114
display id: component2475114
version: 1
top level: BBa_K1697002
definition: LacI
access: public

BBa_K143021
type: Component
persistent identity: component2475109
display id: component2475109
version: 1
top level: BBa_K1697002
definition: RBS-spoVG
access: public

BBa_K143014
type: SequenceAnnotation
persistent identity: annotation2475107
display id: annotation2475107
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475107/range2475107/1
component: BBa_K143014

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type: Range
persistent identity: range2475107
display id: range2475107
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 1
end: 82
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2475109
display id: annotation2475109
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475109/range2475109/1
component: BBa_K143021

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type: Range
persistent identity: range2475109
display id: range2475109
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 83
end: 94
orientation: inline

BBa_K143033
type: SequenceAnnotation
persistent identity: annotation2475114
display id: annotation2475114
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475114/range2475114/1
component: BBa_K143033

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type: Range
persistent identity: range2475114
display id: range2475114
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 95
end: 1180
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2475116
display id: annotation2475116
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475116/range2475116/1
component: BBa_K143021

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type: Range
persistent identity: range2475116
display id: range2475116
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 1181
end: 1192
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2475118
display id: annotation2475118
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475118/range2475118/1
component: BBa_E0040

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type: Range
persistent identity: range2475118
display id: range2475118
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 1193
end: 1912
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2475125
display id: annotation2475125
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475125/range2475125/1
component: BBa_B0015

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type: Range
persistent identity: range2475125
display id: range2475125
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 1913
end: 2041
orientation: inline

BBa_K143015
type: SequenceAnnotation
persistent identity: annotation2475130
display id: annotation2475130
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475130/range2475130/1
component: BBa_K143015

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type: Range
persistent identity: range2475130
display id: range2475130
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 2042
end: 2142
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2475132
display id: annotation2475132
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475132/range2475132/1
component: BBa_K143021

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type: Range
persistent identity: range2475132
display id: range2475132
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 2143
end: 2154
orientation: inline

BBa_K143036
type: SequenceAnnotation
persistent identity: annotation2475137
display id: annotation2475137
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475137/range2475137/1
component: BBa_K143036

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type: Range
persistent identity: range2475137
display id: range2475137
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 2155
end: 3210
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2475139
display id: annotation2475139
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475139/range2475139/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K1697002/annotation2475139/range2475139/1
type: Range
persistent identity: range2475139
display id: range2475139
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 3211
end: 3222
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2475142
display id: annotation2475142
version: 1
top level: BBa_K1697002
location: https://synbiohub.org/public/igem/BBa_K1697002/annotation2475142/range2475142/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1697002/annotation2475142/range2475142/1
type: Range
persistent identity: range2475142
display id: range2475142
version: 1
top level: BBa_K1697002
direction: direction/reverse
start: 3223
end: 3928
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

Pxyl
type: ComponentDefinition
persistent identity: BBa K143014
display id: BBa_K143014
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Xyl for B.subtilis
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 12. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter Xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Promoter xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator '''XylR''', (<bbpart>BBa_K143036</bbpart>) This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter xylose.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter xylose sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A -35
sequence annotation: Sigma A -10
sequence annotation: XylR Operator
sequence: https://synbiohub.org/public/igem/BBa_K143014_sequence/1

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976428
display id: annotation1976428
version: 1
top level: Pxyl
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
type: Range
persistent identity: range1976428
display id: range1976428
version: 1
top level: Pxyl
direction: direction/forward
start: 13
end: 18
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976429
display id: annotation1976429
version: 1
top level: Pxyl
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
type: Range
persistent identity: range1976429
display id: range1976429
version: 1
top level: Pxyl
direction: direction/forward
start: 36
end: 41
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975868
display id: annotation1975868
version: 1
top level: Pxyl
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
type: Range
persistent identity: range1975868
display id: range1975868
version: 1
top level: Pxyl
direction: direction/forward
start: 51
end: 61
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975869
display id: annotation1975869
version: 1
top level: Pxyl
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
type: Range
persistent identity: range1975869
display id: range1975869
version: 1
top level: Pxyl
direction: direction/forward
start: 65
end: 75
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa K143033
display id: BBa_K143033
version: 1
was generated by: iGEM to SBOL conversion
description: LacI (Lva-, N-terminal deletion) regulatory protein
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
owned by: james
owned by: myers
mutable description: LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars. LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''#1(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output ====References==== <biblio> #1 pmid=16166525 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LacI (Lva-, N-terminal deletion) regulatory protein
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143033_sequence/1

LacI (Lva-, N-terminal deletion) regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975974
display id: annotation1975974
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
type: Range
persistent identity: range1975974
display id: range1975974
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 1080
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992702
display id: annotation1992702
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
type: Range
persistent identity: range1992702
display id: range1992702
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994271
display id: annotation1994271
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
type: Range
persistent identity: range1994271
display id: range1994271
version: 1
top level: LacI
direction: direction/forward
start: 1081
end: 1083
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994272
display id: annotation1994272
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
type: Range
persistent identity: range1994272
display id: range1994272
version: 1
top level: LacI
direction: direction/forward
start: 1084
end: 1086
orientation: inline

XylR
type: ComponentDefinition
persistent identity: BBa K143036
display id: BBa_K143036
version: 1
was generated by: iGEM to SBOL conversion
description: Xylose operon regulatory protein
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The XylR protein was PCR cloned form the ''B. subtilis'' genome using Pfu DNA polymerase
owned by: james
owned by: myers
mutable description: Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as xylose. XylR if the regulator protein for the Xylose operon in ''B. subtilis''#1 and is responsible for ensuring that in the absence of xylose the xylose metabolism proteins are not expressed. Though endogenous to ''B. subtilis'', to minimise the leakage of a xylose inducible promoter XylR should be over-expressed. In the presence of xylose, the XylR tetramer is unable to bind DNA and so transcription resumes. It must be noted that in all ''B. subtilis'' strains that do not have the Xylose operon knocked out '''the xylose inducer will gradually be metabolised by the host''' XylR was used in conjunction with the '''Xylose operon promoter''' (<bbpart>BBa_K143014<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output
discontinued: false
dominant: true
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The XylR protein was identified in the genome using its Genbank entry#2 and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Xylose operon regulatory protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143036_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1992711
display id: annotation1992711
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_K143036/annotation1992711/range1992711/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K143036/annotation1992711/range1992711/1
type: Range
persistent identity: range1992711
display id: range1992711
version: 1
top level: XylR
direction: direction/forward
start: 1
end: 3
orientation: inline

Xylose operon regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975975
display id: annotation1975975
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_K143036/annotation1975975/range1975975/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143036/annotation1975975/range1975975/1
type: Range
persistent identity: range1975975
display id: range1975975
version: 1
top level: XylR
direction: direction/forward
start: 1
end: 1050
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992712
display id: annotation1992712
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_K143036/annotation1992712/range1992712/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143036/annotation1992712/range1992712/1
type: Range
persistent identity: range1992712
display id: range1992712
version: 1
top level: XylR
direction: direction/forward
start: 1051
end: 1053
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992713
display id: annotation1992713
version: 1
top level: XylR
location: https://synbiohub.org/public/igem/BBa_K143036/annotation1992713/range1992713/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143036/annotation1992713/range1992713/1
type: Range
persistent identity: range1992713
display id: range1992713
version: 1
top level: XylR
direction: direction/forward
start: 1054
end: 1056
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

Ph-s
type: ComponentDefinition
persistent identity: BBa K143015
display id: BBa_K143015
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter hyper-spank for B. subtilis
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 123. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K413035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter hyper-spank sequence.
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI Operator
sequence annotation: Sigma A -35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143015_sequence/1

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976423
display id: annotation1976423
version: 1
top level: Ph-s
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
type: Range
persistent identity: range1976423
display id: range1976423
version: 1
top level: Ph-s
direction: direction/forward
start: 10
end: 30
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976424
display id: annotation1976424
version: 1
top level: Ph-s
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
type: Range
persistent identity: range1976424
display id: range1976424
version: 1
top level: Ph-s
direction: direction/forward
start: 46
end: 50
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976425
display id: annotation1976425
version: 1
top level: Ph-s
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
type: Range
persistent identity: range1976425
display id: range1976425
version: 1
top level: Ph-s
direction: direction/forward
start: 69
end: 74
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976426
display id: annotation1976426
version: 1
top level: Ph-s
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
type: Range
persistent identity: range1976426
display id: range1976426
version: 1
top level: Ph-s
direction: direction/forward
start: 81
end: 101
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1697002_sequence/1
type: Sequence
persistent identity: BBa K1697002 sequence
display id: BBa_K1697002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtgaaaaggtggtgaaatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataaaaaggtggtgaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143014_sequence/1
type: Sequence
persistent identity: BBa K143014 sequence
display id: BBa_K143014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143033_sequence/1
type: Sequence
persistent identity: BBa K143033 sequence
display id: BBa_K143033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143036_sequence/1
type: Sequence
persistent identity: BBa K143036 sequence
display id: BBa_K143036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactggattaaataaatcaactgtctcatcacaggtaaacacgttaatgaaagaaagtatggtatttgaaataggtcaaggacaatcaagtggcggaagaagacctgtcatgcttgtttttaataaaaaggcaggatactccgttggaatagatgttggtgtggattatattaatggcattttaacagaccttgaaggaacaatcgttcttgatcaataccgccatttggaatccaattctccagaaataacgaaagacattttgattgatatgattcatcactttattacgcaaatgccccaatctccgtacgggtttattggtataggtatttgcgtgcctggactcattgataaagatcaaaaaattgttttcactccgaactccaactggagagatattgacttaaaatcttcgatacaagagaagtacaatgtgtctgtttttattgaaaatgaggcaaatgctggcgcatatggagaaaaactatttggagctgcaaaaaatcacgataacattatttacgtaagtatcagcacaggaatagggatcggtgttattatcaacaatcatttatatagaggagtaagcggcttctctggagaaatgggacatatgacaatagactttaatggtcctaaatgcagttgcggaaaccgaggatgctgggaattgtatgcttcagagaaggctttattaaaatctcttcagaccaaagagaaaaaactgtcctatcaagatatcataaacctcgcccatctgaatgatatcggaaccttaaatgcattacaaaattttggattctatttaggaataggccttaccaatattctaaatactttcaacccacaagccgtaattttaagaaatagcataattgaatcgcatcctatggttttaaattcaatgagaagtgaagtatcatcaagggtttattcccaattaggcaatagctatgaattattgccatcttccttaggacagaatgcaccggcattaggaatgtcctccattgtgattgatcattttctggacatgattacaatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143015_sequence/1
type: Sequence
persistent identity: BBa K143015 sequence
display id: BBa_K143015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagggtaaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z