BBa_K1698001 1 BBa_K1698001 BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp 2015-09-17T11:00:00Z 2015-09-22T03:41:26Z Target sequence comes from HPV-16 Genome : NC_001526 Part may be activated by established protocol to become a detector plasmid for a 55bp Target sequence, found on the HPV L1 Capsid gene. For protocol for construction of detector by digestion of plasmid see HERE The Target is located in the HPV genome flanked by HaeIII restriction sites. Detector may work best if genomic sample first digested with this enzyme to create target fragment of the correct length. The part was cloned into a plasmid containing BBa_K584001 to allow examination of cells following successful transformation using a fluorescent microscope. Also, presence of GFP expressing part meant that detector colonies could be differentiated from control colonies. For more information see (http://2015.igem.org/Team:Cork_Ireland/Project#standard) The sequence of this part with K584001 biobrick part may be found on the Registry of Standard Parts false false _2116_ 20076 22006 9 false Target sequence is ideally chosen between two restriction sites to allow digestion of a large genomic sample to yield target fragment of correct size. Also, the size of the target was chosen as it works efficiently with detector. false Leanne O Sullivan BBa_K1698001_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatagctcttcacctgtgtaggtgttgaggtaggtcgtgggtaccaataataaaagcttcagccattaggtgtgggcattagtggcactgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z