BBa_K1698001
1
BBa_K1698001
BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp
2015-09-17T11:00:00Z
2015-09-22T03:41:26Z
Target sequence comes from HPV-16 Genome : NC_001526
Part may be activated by established protocol to become a detector plasmid for a 55bp Target sequence, found on the HPV L1 Capsid gene.
For protocol for construction of detector by digestion of plasmid see HERE
The Target is located in the HPV genome flanked by HaeIII restriction sites. Detector may work best if genomic sample first digested with this enzyme to create target fragment of the correct length.
The part was cloned into a plasmid containing BBa_K584001 to allow examination of cells following successful transformation using a fluorescent microscope. Also, presence of GFP expressing part meant that detector colonies could be differentiated from control colonies. For more information see (http://2015.igem.org/Team:Cork_Ireland/Project#standard)
The sequence of this part with K584001 biobrick part may be found on the Registry of Standard Parts
false
false
_2116_
20076
22006
9
false
Target sequence is ideally chosen between two restriction sites to allow digestion of a large genomic sample to yield target fragment of correct size. Also, the size of the target was chosen as it works efficiently with detector.
false
Leanne O Sullivan
BBa_K1698001_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatagctcttcacctgtgtaggtgttgaggtaggtcgtgggtaccaataataaaagcttcagccattaggtgtgggcattagtggcactgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z