LOCUS       BBa_K1699004             359 bp    DNA     linear       22-Nov-2024
DEFINITION  gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter
ACCESSION   BBa_K1699004
VERSION     BBa_K1699004.1  
FEATURES             Location/Qualifiers
     promoter        1..249
                     /label=Human U6 promoter
     misc_feature    257..278
                     /label=gRNA-UBB-Exon 2
     misc_feature    279..359
                     /label=gRNA scaffold for SaCas9
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca ccggtgtttc tgactacaac atccagagtt ttagtactct ggaaacagaa
      301 tctactaaaa caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg agatttttt
//