LOCUS       BBa_K1699005             359 bp    DNA     linear       16-Feb-2025
DEFINITION  gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm 
            under U6 promoter
ACCESSION   BBa_K1699005
VERSION     BBa_K1699005.1  
FEATURES             Location/Qualifiers
     promoter        1..249
                     /label=Human U6 promoter
     misc_feature    256..275
                     /label=gRNA for pMLPm
     misc_feature    276..359
                     /label=gRNA scaffold for SpCas9
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca ccggtgtact gtatgtacat acagtgtttt agagctagaa atagcaagtt
      301 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttt
//