LOCUS       BBa_K1700000             820 bp    DNA     linear       16-Feb-2025
DEFINITION  Use toehold to regulate GFP
ACCESSION   BBa_K1700000
VERSION     BBa_K1700000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..100
                     /label=toehold
     misc_feature    156..158
                     /label=ATG start codon
     CDS             101..820
                     /label=GFP coding sequence
     misc_feature    818..820
                     /label=TAA stop codon
ORIGIN
        1 gggagtacat catctatact gtagttatag ttatgagaca agaacaagga gacataacat
       61 gaactacagt aacgtagtta acctggcggc agcgcaaaag atgcgtaaag gagaagaact
      121 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      181 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      241 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      301 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      361 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      421 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      481 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      541 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      601 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      661 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      721 ttcgaaagat cccaacgaaa agagagatca catggtcctt cttgagtttg taacagctgc
      781 tgggattaca catggcatgg atgaactata caaataataa
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