BBa_K1700000 1 BBa_K1700000 Use toehold to regulate GFP 2015-09-16T11:00:00Z 2015-09-17T09:57:13Z We synthesis the part. The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest. The trigger RNA of our toehold is miRNA-144.When adding it to the system, GFP can be expressed. false false _2118_ 28512 28512 9 false Toehold can be open by trigger RNA, thus letting GFP express. false Meixi Liu annotation2467633 1 TAA stop codon range2467633 1 818 820 annotation2467606 1 toehold range2467606 1 1 100 annotation2467629 1 ATG start codon range2467629 1 156 158 annotation2467632 1 GFP coding sequence range2467632 1 101 820 BBa_K1700000_sequence 1 gggagtacatcatctatactgtagttatagttatgagacaagaacaaggagacataacatgaactacagtaacgtagttaacctggcggcagcgcaaaagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z