LOCUS       BBa_K1700001            2776 bp    DNA     linear       23-Dec-2024
DEFINITION  toehold T3 RNAP
ACCESSION   BBa_K1700001
VERSION     BBa_K1700001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..124
                     /label=toehold
     misc_feature    150..152
                     /label=ATG start codon
     CDS             150..2776
                     /label=T3 RNAP
     misc_feature    2774..2776
                     /label=TAA stop codon
ORIGIN
        1 gtaccccggg ggtcatgctg tttcatatga tctgggtatc tcgcaaagtt atagttatga
       61 gacaagaaca gaggagacat aacatgaact ttgcgtctaa agttaacctg gcggcagcgc
      121 aaaaaacatc atcgaaaaca tcgaaaagaa tgacttctca gaaatcgaac tggctgctat
      181 cccgttcaac acactggctg accactacgg aagcgccttg gctaaagagc agttggcttt
      241 agaacatgag tcttatgagc taggcgagcg ccgcttcctc aagatgcttg agcgtcaagc
      301 gaaagctggt gagattgcag acaacgcagc cgctaagccg ttactcgcta cgcttctccc
      361 taagttaacc acacgtatcg tcgagtggct cgaagagtac gcatcgaaga aaggccgcaa
      421 gcctagcgca tacgcaccgc tccagttact caagccggag gcctccgcgt ttatcaccct
      481 gaaagttatc cttgcgtcac taaccagtac gaacatgaca accattcagg ccgctgctgg
      541 tatgctgggg aaagccattg aggacgaggc acgatttggg cgcatccgtg acctagaagc
      601 gaagcacttc aagaagcacg ttgaggaaca gcttaacaag cgccacgggc aagtctacaa
      661 gaaagcattt atgcaggtgg tcgaggccga tatgattggt cgaggtctgc ttggtggcga
      721 ggcgtggtct agctgggata aagaaaccac gatgcacgta gggattcgcc tgattgaaat
      781 gctgattgaa tccacgggtc tggtggaatt acagcgccac aacgcaggta acgcaggctc
      841 tgaccatgag gcactgcaac tggcccaaga gtacgtggac gtattagcga agcgtgcagg
      901 cgctctggcg ggtatctctc cgatgttcca gccgtgtgtc gtaccgccga aaccttgggt
      961 agcaatcaca gggggcggct attgggctaa cggtcgcaga cctttggcac tcgttcgcac
     1021 tcactctaag aagggcttga tgcgctacga agacgtttac atgccagaag tctacaaggc
     1081 tgtgaacctc gcgcaaaaca ccgcatggaa aatcaacaag aaagttcttg ctgttgtcaa
     1141 tgagattgtt aactggaaga attgcccggt agcagacatt ccatcgctgg agcgccaaga
     1201 gttaccgcct aagcctgacg acattgacac caacgaggca gcgctcaagg agtggaagaa
     1261 agccgctgct ggtatctatc gcttggacaa ggcacgagtg tctcgccgta tcagcttaga
     1321 gttcatgctg gagcaggcca acaagttcgc aagtaagaaa gcaatctggt tcccttacaa
     1381 catggactgg cgcggtcgtg tgtacgctgt gccgatgttc aacccgcaag gcaacgacat
     1441 gacgaaaggt ctgctgaccc ttgctaaagg caagccaatc ggtgaggaag gtttctactg
     1501 gctgaaaatc cacggtgcga actgtgcggg tgttgataag gttccattcc cggagcgcat
     1561 cgcgttcatt gagaagcacg tagacgacat tctggcttgc gctaaagacc caatcaataa
     1621 cacttggtgg gctgagcagg attcaccgtt ctgtttcctc gcgttttgct tcgagtatgc
     1681 aggcgttacg caccacggtc tgagctacaa ttgctctctg ccgctggcgt tcgacgggtc
     1741 ttgctctggt atccagcact tctccgcgat gctccgcgat gaggtaggcg gtcgtgcggt
     1801 taacctgctg ccaagcgaaa ccgtgcagga catttacggc atcgttgcac agaaagtaaa
     1861 cgagattctc aaacaggatg caatcaacgg cacgcctaac gagatgatta ccgtgaccga
     1921 caaggacacc ggggaaatct cagagaagct caaacttgga acctcaacgc tggcgcaaca
     1981 gtggctggca tatggtgtaa cccgtagcgt aactaaacgt tcggtcatga cgctggctta
     2041 cggttccaag gagttcggct ttcgtcaaca ggtattggat gacaccattc agcctgcaat
     2101 tgacagcggt aagggcttga tgttcaccca accgaaccaa gcggctggct atatggctaa
     2161 gctgatttgg gatgcggtaa gcgtgaccgt agttgcagcg gttgaggcga tgaactggct
     2221 caaatctgcc gctaagctgc tggctgctga ggtcaaggac aagaagacca aggagattct
     2281 gcgccaccgt tgcgcggttc actggactac gccggacggc ttcccggtct ggcaggaata
     2341 ccgcaagcca ctccagaagc gtctcgatat gattttctta gggcaattcc gtctgcaacc
     2401 gacgattaat accctcaagg attcaggcat tgacgcacac aagcaggagt ctggcatcgc
     2461 tcctaacttt gttcactcac aggacggtag ccacctccgc atgacagtcg tttatgctca
     2521 cgagaagtat ggcattgagt cctttgcgct catccatgac agctttggga ctatcccggc
     2581 agacgctggt aagctcttta aggctgtgcg tgaaacgatg gttatcacct atgagaacaa
     2641 cgatgtgctg gcagacttct actctcagtt tgccgaccag ctacacgaga cgcaactgga
     2701 caagatgcct ccgcttccga agaaaggaaa cctgaacctg caagacattc tcaagtctga
     2761 ctttgccttt gcataa
//